CD184 / CXCR4 antibody
Rabbit anti Human CD184 / CXCR4 antibody recognizes the human G-protein-coupled chemokine receptor CD184, also known as CXCR4. CD184 is a 352 amino acid ~40 kDa cell surface protein known to act as a co-factor in HIV infection of CD4+ T cells (Bleul et al. 1997). Mutations in the CD184 gene are associated with WHIM syndrome (WHIMS), an inherited combined immunodeficiency disease (Taniuchi et al..).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to human CD184 / CXCR4 were raised by repeated immunisation of rabbits with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Peptide sequence corresponding to amino acids 1-14 of human CD184 / CXCR4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P61073
- Entrez Gene
- CXCR4
- GO Terms
- GO:0005886 plasma membrane
- GO:0000187 activation of MAPK activity
- GO:0001666 response to hypoxia
- GO:0003779 actin binding
- GO:0009615 response to virus
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0006954 inflammatory response
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0009986 cell surface
- GO:0015026 coreceptor activity
- GO:0016023 cytoplasmic membrane-bounded vesicle
- GO:0016493 C-C chemokine receptor activity
- GO:0016494 C-X-C chemokine receptor activity
- GO:0019059 initiation of viral infection
- GO:0030260 entry into host cell
- GO:0031252 cell leading edge
- GO:0031625 ubiquitin protein ligase binding
- GO:0032027 myosin light chain binding
- GO:0043130 ubiquitin binding
- GO:0050920 regulation of chemotaxis
- Regulatory
- For research purposes only
Applications of CD184 / CXCR4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | 1/1000 | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for CD184 / CXCR4 antibody
-
Nguyen, D.H. and Taub, D. (2002) CXCR4 function requires membrane cholesterol: implications for HIV infection.
J Immunol. 168: 4121-6. -
Zaitseva, M. et al. (2005) Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association.
J Leukoc Biol. 78: 1306-17. -
Moll, N.M. et al. (2009) Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis.
Arch Neurol. 66: 44-53. -
Rubie, C. et al. (2008) Differential CXC receptor expression in colorectal carcinomas.
Scand J Immunol. 68: 635-44. -
Rubie, C. et al. (2006) Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.
World J Gastroenterol. 12: 6627-33. -
Oliveira Frick, V. et al. (2011) Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies.
Tumour Biol. 32: 189-96. -
Omland, S.H. et al. (2017) Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin.
BMC Cancer. 17 (1): 675.
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