CD184 / CXCR4 antibody

Rabbit anti Human CD184 / CXCR4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD184 / CXCR4
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP442
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.1 mg loader
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loader
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Rabbit anti Human CD184 / CXCR4 antibody recognizes the human G-protein-coupled chemokine receptor CD184, also known as CXCR4. CD184 is a 352 amino acid ~40 kDa cell surface protein known to act as a co-factor in HIV infection of CD4+ T cells (Bleul et al. 1997). Mutations in the CD184 gene are associated with WHIM syndrome (WHIMS), an inherited combined immunodeficiency disease (Taniuchi et al..).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antisera to human CD184 / CXCR4 were raised by repeated immunisation of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Peptide sequence corresponding to amino acids 1-14 of human CD184 / CXCR4.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1/1000 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD184 / CXCR4 antibody

  1. Nguyen, D.H. and Taub, D. (2002) CXCR4 function requires membrane cholesterol: implications for HIV infection.
    J Immunol. 168: 4121-6.
  2. Zaitseva, M. et al. (2005) Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association.
    J Leukoc Biol. 78: 1306-17.
  3. Moll, N.M. et al. (2009) Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis.
    Arch Neurol. 66: 44-53.
  4. Rubie, C. et al. (2008) Differential CXC receptor expression in colorectal carcinomas.
    Scand J Immunol. 68: 635-44.
  5. Rubie, C. et al. (2006) Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.
    World J Gastroenterol. 12: 6627-33.
  6. Oliveira Frick, V. et al. (2011) Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies.
    Tumour Biol. 32: 189-96.
  7. Omland, S.H. et al. (2017) Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin.
    BMC Cancer. 17 (1): 675.

Immunofluorescence

RRID
AB_322743
UniProt
P61073
Entrez Gene
CXCR4
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0001666 response to hypoxia
GO:0003779 actin binding
GO:0009615 response to virus
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0009986 cell surface
GO:0015026 coreceptor activity
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0016493 C-C chemokine receptor activity
GO:0016494 C-X-C chemokine receptor activity
GO:0019059 initiation of viral infection
GO:0030260 entry into host cell
GO:0031252 cell leading edge
GO:0031625 ubiquitin protein ligase binding
GO:0032027 myosin light chain binding
GO:0043130 ubiquitin binding
GO:0050920 regulation of chemotaxis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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