Rabbit anti Human CD184 / CXCR4 antibody
recognizes the human G-protein-coupled chemokine receptor CD184, also known as CXCR4. CD184 is a 352 amino acid ~40 kDa cell surface protein known to act as a co-factor in HIV infection of CD4+
T cells (Bleul et al. 1997
). Mutations in the CD184 gene are associated with WHIM syndrome (WHIMS
), an inherited combined immunodeficiency disease (Taniuchi et al..
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography
- Antiserum Preparation
- Antisera to human CD184 / CXCR4 were raised by repeated immunisation of rabbits with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Peptide sequence corresponding to amino acids 1-14 of human CD184 / CXCR4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
response to hypoxia
response to virus
integral to membrane
elevation of cytosolic calcium ion concentration
cytoplasmic membrane-bounded vesicle
C-C chemokine receptor activity
C-X-C chemokine receptor activity
initiation of viral infection
entry into host cell
cell leading edge
ubiquitin protein ligase binding
myosin light chain binding
regulation of chemotaxis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD184 / CXCR4 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for CD184 / CXCR4 antibody
Nguyen, D.H. and Taub, D. (2002) CXCR4 function requires membrane cholesterol: implications for HIV infection.
J Immunol. 168: 4121-6.
Zaitseva, M. et al. (2005) Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association.
J Leukoc Biol. 78: 1306-17.
Moll, N.M. et al. (2009) Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis.
Arch Neurol. 66: 44-53.
Rubie, C. et al. (2008) Differential CXC receptor expression in colorectal carcinomas.
Scand J Immunol. 68: 635-44.
Rubie, C. et al. (2006) Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.
World J Gastroenterol. 12: 6627-33.
Oliveira Frick, V. et al. (2011) Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies.
Tumour Biol. 32: 189-96.
Omland, S.H. et al. (2017) Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin.
BMC Cancer. 17 (1): 675.
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