Rabbit anti Human CD184 / CXCR4 antibody
recognizes the human G-protein-coupled chemokine receptor CD184, also known as CXCR4. CD184 is a 352 amino acid ~40 kDa cell surface protein known to act as a co-factor in HIV infection of CD4+
T cells (Bleul et al. 1997
). Mutations in the CD184 gene are associated with WHIM syndrome (WHIMS
), an inherited combined immunodeficiency disease (Taniuchi et al..
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to human CD184 / CXCR4 were raised by repeated immunisation of rabbits with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Peptide sequence corresponding to amino acids 1-14 of human CD184 / CXCR4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
response to hypoxia
response to virus
integral to membrane
elevation of cytosolic calcium ion concentration
cytoplasmic membrane-bounded vesicle
C-C chemokine receptor activity
C-X-C chemokine receptor activity
initiation of viral infection
entry into host cell
cell leading edge
ubiquitin protein ligase binding
myosin light chain binding
regulation of chemotaxis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD184 / CXCR4 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for CD184 / CXCR4 antibody
Nguyen, D.H. and Taub, D. (2002) CXCR4 function requires membrane cholesterol: implications for HIV infection.
J Immunol. 168: 4121-6.
Zaitseva, M. et al. (2005) Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association.
J Leukoc Biol. 78: 1306-17.
Moll, N.M. et al. (2009) Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis.
Arch Neurol. 66: 44-53.
Rubie, C. et al. (2008) Differential CXC receptor expression in colorectal carcinomas.
Scand J Immunol. 68: 635-44.
Rubie, C. et al. (2006) Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.
World J Gastroenterol. 12: 6627-33.
Oliveira Frick, V. et al. (2011) Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies.
Tumour Biol. 32: 189-96.
Omland, S.H. et al. (2017) Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin.
BMC Cancer. 17 (1): 675.
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