CD184 / CXCR4 antibody

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Anti Human CD184 / CXCR4 Antibody thumbnail image 2

Anti Human CD184 / CXCR4 Antibody gallery image 1 — Western Blot analysis of HeLa whole cell lysate probed with Rabbit anti Human CD184 / CXCR4 (**AHP442**).

Anti Human CD184 / CXCR4 Antibody gallery image 2 — Immunocytochemical staining of HeLa cells with Rabbit anti Human CD184 / CXCR4 (**AHP442**).

Rabbit anti Human CD184 / CXCR4

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP442 Datasheet	C F IP WB SDS	0.1 mg	Log in		Get Quote

Rabbit anti Human CD184 / CXCR4 antibody recognizes the human G-protein-coupled chemokine receptor CD184, also known as CXCR4. CD184 is a 352 amino acid ~40 kDa cell surface protein known to act as a co-factor in HIV infection of CD4⁺ T cells (Bleul et al. 1997). Mutations in the CD184 gene are associated with WHIM syndrome (WHIMS), an inherited combined immunodeficiency disease (Taniuchi et al..).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography
Antiserum Preparation: Antisera to human CD184 / CXCR4 were raised by repeated immunisation of rabbits with highly purified antigen.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
Immunogen: Peptide sequence corresponding to amino acids 1-14 of human CD184 / CXCR4.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P61073
Entrez Gene: CXCR4
GO Terms: GO:0005886 plasma membrane; GO:0000187 activation of MAPK activity; GO:0001666 response to hypoxia; GO:0003779 actin binding; GO:0009615 response to virus; GO:0006915 apoptosis; GO:0016021 integral to membrane; GO:0006954 inflammatory response; GO:0007204 elevation of cytosolic calcium ion concentration; GO:0009986 cell surface; GO:0015026 coreceptor activity; GO:0016023 cytoplasmic membrane-bounded vesicle; GO:0016493 C-C chemokine receptor activity; GO:0016494 C-X-C chemokine receptor activity; GO:0019059 initiation of viral infection; GO:0030260 entry into host cell; GO:0031252 cell leading edge; GO:0031625 ubiquitin protein ligase binding; GO:0032027 myosin light chain binding; GO:0043130 ubiquitin binding; GO:0050920 regulation of chemotaxis
Regulatory: For research purposes only

Applications of CD184 / CXCR4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting	1/1000	1/2000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Application Based External Images

Immunofluorescence

Product Specific References

References for CD184 / CXCR4 antibody

Nguyen, D.H. and Taub, D. (2002) CXCR4 function requires membrane cholesterol: implications for HIV infection.
J Immunol. 168: 4121-6.
Zaitseva, M. et al. (2005) Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association.
J Leukoc Biol. 78: 1306-17.
Moll, N.M. et al. (2009) Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis.
Arch Neurol. 66: 44-53.
Rubie, C. et al. (2008) Differential CXC receptor expression in colorectal carcinomas.
Scand J Immunol. 68: 635-44.
Rubie, C. et al. (2006) Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.
World J Gastroenterol. 12: 6627-33.
Oliveira Frick, V. et al. (2011) Changes in CXCL12/CXCR4-chemokine expression during onset of colorectal malignancies.
Tumour Biol. 32: 189-96.
Omland, S.H. et al. (2017) Cancer associated fibroblasts (CAFs) are activated in cutaneous basal cell carcinoma and in the peritumoural skin.
BMC Cancer. 17 (1): 675.

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