CD117 (pTyr721) antibody

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Rabbit anti CD117 (pTyr721)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2566 WB 50 µl
Rabbit anti CD117 (pTyr721) antibody recognizes CD117, also known as proto-oncogene c-kit, mast/stem cell growth factor receptor Kit, Piebald trait protein and v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, when phosphorylated at tyrosine 721. CD117 is a member of the tyrosine protein kinase family and is the receptor for stem cell factor (SCF).

Upon binding of SCF, CD117 activates a number of cell signaling pathways including MAP kinase, PI3K and JAK/STAT pathways (Laing et al. 2013).

SCF binding induces autophosphorylation of CD117 at tyrosine 568, tyrosine 570, tyrosine 721 and tyrosine 730. Phosphorylation of tyrosine 721 of CD117 is important for the binding and activation of PI-3 kinase p85 subunit alpha (Lennartsson and Rönnstrand, 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to CD117 (pTyr721) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 721 of human CD117

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P10721 Related reagents
Entrez Gene
KIT Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0008584 male gonad development
Regulatory
For research purposes only

Applications of CD117 (pTyr721) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Liang, J. et al. (2013) The C-kit receptor-mediated signal transduction and tumor-related diseases
    Int J Biol Sci. 9 (5): 435-43.
  2. Lennartsson, J. & Rönnstrand, L. (2012) Stem cell factor receptor/c-Kit: from basic science to clinical implications
    Physiol Rev. 92 (4): 1619-49.