CD117 (pTyr721) antibody
Rabbit anti CD117 (pTyr721)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CD117 (pTyr721)
Antibody Quality and Performance Guarantee
| Rabbit anti CD117 (pTyr721) antibody recognizes CD117, also known as proto-oncogene c-kit, mast/stem cell growth factor receptor Kit, Piebald trait protein and v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, when phosphorylated at tyrosine 721. CD117 is a member of the tyrosine protein kinase family and is the receptor for stem cell factor (SCF). Upon binding of SCF, CD117 activates a number of cell signaling pathways including MAP kinase, PI3K and JAK/STAT pathways (Laing et al. 2013). SCF binding induces autophosphorylation of CD117 at tyrosine 568, tyrosine 570, tyrosine 721 and tyrosine 730. Phosphorylation of tyrosine 721 of CD117 is important for the binding and activation of PI-3 kinase p85 subunit alpha (Lennartsson and Rönnstrand, 2012). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to CD117 (pTyr721) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 721 of human CD117
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Liang, J. et al. (2013) The C-kit receptor-mediated signal transduction and tumor-related diseases
Int J Biol Sci. 9 (5): 435-43. -
Lennartsson, J. & Rönnstrand, L. (2012) Stem cell factor receptor/c-Kit: from basic science to clinical implications
Physiol Rev. 92 (4): 1619-49.
- UniProt
- P10721
- Entrez Gene
- KIT
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005524 ATP binding
- GO:0005615 extracellular space
- GO:0008584 male gonad development
AHP2566
141528
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