Catenin Alpha 1 antibody
Goat anti Catenin Alpha 1
Catenin alpha1 antibody recognizes catenin alpha-1, also known as alpha E-catenin, cadherin-associated protein or renal carcinoma antigen NY-REN-13. The antibody has been extensively validated for western blotting using whole cell lysates.
Catenin Alpha 1 Antibody with Control Lysate
Catenin alpha1 antibody recognizes catenin alpha-1, also known as alpha E-catenin, cadherin-associated protein or renal carcinoma antigen NY-REN-13. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00116 | WB | 100 µl | ||||
| VPA00116KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human catenin alpha 1 antibody recognizes catenin alpha 1, also known as alpha E-catenin, cadherin-associated protein or renal carcinoma antigen NY-REN-13.
Goat anti Human catenin alpha 1 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Goat anti Human catenin alpha 1 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti catenin Alpha 1 detects a band of approximately 102 kDa in A431 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C-KREKQDETQTKIK, from the internal region of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A431 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P35221 Related reagents
- Entrez Gene
- CTNNA1 Related reagents
- GO Terms
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0005198 structural molecule activity
- GO:0005911 cell-cell junction
- GO:0005912 adherens junction
- GO:0008013 beta-catenin binding
- GO:0015629 actin cytoskeleton
- GO:0016342 catenin complex
- GO:0017166 vinculin binding
- GO:0034332 adherens junction organization
- GO:0042692 muscle cell differentiation
- GO:0043297 apical junction assembly
- GO:0045295 gamma-catenin binding
- GO:0045296 cadherin binding
- GO:0051149 positive regulation of muscle cell differentiation
- GO:0071681 cellular response to indole-3-methanol
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Catenin Alpha 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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