Caspase-9 (Active) antibody

Rabbit anti Caspase-9 (Active)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-9 (Active)

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AHP2288
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SDS Safety Datasheet SDS
IP WB 50 µg loader
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Rabbit anti caspase-9 antibody recognizes caspase-9 also known as apoptotic protease-activating factor 3 (APAF-3). Caspase-9 as the name implies is a member of the cysteine-aspartic acid protease family.

The active form of caspase-9 is generated via cleavage of the pro-caspase-9 protein into two fragments, known as the large and the small fragment. The trigger for caspase-9 cleavage is the binding of cytochrome c to pro-caspase-9, which is released upon receiving apoptotic stimuli.

Caspase-9 is an effector caspase that plays a role in the mediation of mitochondrial apoptosis. Active caspase-9 cleaves pro-caspases 3 and 7 into their active forms. Caspase activity can be inhibited by c-IAP1, c-IAP2, XIAP and other members of the inhibitor of apoptosis protein family.

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% Glycerol
0.5% Bovine serum Albumin
0.02% Thiomersal
Immunogen
Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 10 20 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti caspase-9 recognizes the cleaved form of caspase 9 (approximately 37kDa) in Jurkat cells treated with etoposide

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Synonyms
Apoptotic PROTEASE-ACTIVATING Factor 3
CASP-9
UniProt
P55211
Entrez Gene
CASP9
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005829 cytosol
GO:0006508 proteolysis
GO:0008047 enzyme activator activity
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0048015 phosphatidylinositol-mediated signaling
GO:0048011 nerve growth factor receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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