Caspase-4 antibody

Rabbit anti Caspase-4 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-4
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP964
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P WB 0.1 mg loader
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Rabbit anti caspase-4 (N-terminal) antibody recognizes an epitope within the N-terminal region (NT) of Caspase-4, otherwise known as ICH-2. Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits.

Caspase-4 is localized to the endoplasmic reticulum (ER) membrane and is involved in response to ER membrane stress in conditions including Alzheimer’s disease and Ischemia and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human Caspase-4 (NT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 16 amino acid peptide from human Caspase-4 amino terminus.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 2.0ug/ml
Western Blotting 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
*This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Spleen tissue.
Western Blotting
AHP964 detects a band of approximately 34 kDa in Ramos cell lysates.

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References for Caspase-4 antibody

  1. Katayama, T. et al. (2004) Induction of neuronal death by ER stress in Alzheimer's disease.
    J Chem Neuroanat. 28 (1-2): 67-78.

Further Reading

  1. Kamens, J. et al. (1995) Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases.
    J Biol Chem. 270 (25): 15250-6.
  2. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
    Biochem J. 326 ( Pt 1): 1-16.

Synonyms
ICH-2
RRID
AB_2070198
UniProt
P49662
Entrez Gene
CASP4
GO Terms
GO:0005515 protein binding
GO:0005622 intracellular
GO:0004197 cysteine-type endopeptidase activity
GO:0006915 apoptosis
GO:0006508 proteolysis
GO:0006917 induction of apoptosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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