Carabin antibody
Rabbit anti Human Carabin antibody recognizes the N-terminal region of human Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins.
Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway and acts as an inhibitor of the Ras signaling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras.
Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. Rabbit anti Human Carabin antibody (AHP1486) does not cross react with either TBC1D10A or TBC1D10B.
Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Studies demonstrate that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway and acts as an inhibitor of the Ras signaling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras.
Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. Rabbit anti Human Carabin antibody (AHP1486) does not cross react with either TBC1D10A or TBC1D10B.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human Carabin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A 17 amino acid peptide from near the amino terminus of human Carabin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8IV04
- Entrez Gene
- TBC1D10C
- GO Terms
- GO:0005622 intracellular
- GO:0005097 Rab GTPase activator activity
- GO:0032313 regulation of Rab GTPase activity
- Regulatory
- For research purposes only
Applications of Carabin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 1 | 2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1486 detects a band of approximately 55kDa in human spleen tissue cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Pan, F. et al. (2007) Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin.
Nature. 445 (7126): 433-6. -
Weil, R. & Israël, A. (2006) Deciphering the pathway from the TCR to NF-kappaB.
Cell Death Differ. 13 (5): 826-33. -
Im, S.H. & Rao, A. (2004) Activation and deactivation of gene expression by Ca2+/calcineurin-NFAT-mediated signaling.
Mol Cells. 18 (1): 1-9. -
White, R.A. et al. (2000) The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, BUB2, and cdc16 maps to mouse chromosome 5 and human chromosome 4.
Cytogenet Cell Genet. 89 (3-4): 272-5.
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