Carabin antibody

Rabbit anti Carabin (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Carabin
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1486
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P * WB 0.1 mg loader
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Rabbit anti Human Carabin antibody recognizes the N-terminal region of human Carabin, also known as TBC1 domain family member 10C (TBC1D10C), a 50 kDa protein belonging to the TBC1 domain family of proteins.

Carabin differs from other members of the TBC1 domain protein family, which appear to play a role in the regulation of cell growth and differentiation. Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway and acts as an inhibitor of the Ras signaling pathway. Carabin is also believed to mediate crosstalk between calcineurin and Ras.

Carabin is most abundantly expressed in spleen and peripheral blood leucocytes. Rabbit anti Human Carabin antibody (AHP1486) does not cross react with either TBC1D10A or TBC1D10B.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Carabin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 17 amino acid peptide from near the amino terminus of human Carabin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1486 detects a band of approximately 55kDa in human spleen tissue cell lysates.

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Further Reading

  1. Pan, F. et al. (2007) Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin.
    Nature. 445 (7126): 433-6.
  2. Weil, R. & Israël, A. (2006) Deciphering the pathway from the TCR to NF-kappaB.
    Cell Death Differ. 13 (5): 826-33.
  3. Im, S.H. & Rao, A. (2004) Activation and deactivation of gene expression by Ca2+/calcineurin-NFAT-mediated signaling.
    Mol Cells. 18 (1): 1-9.
  4. White, R.A. et al. (2000) The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, BUB2, and cdc16 maps to mouse chromosome 5 and human chromosome 4.
    Cytogenet Cell Genet. 89 (3-4): 272-5.

RRID
AB_2199227
UniProt
Q8IV04
Entrez Gene
TBC1D10C
GO Terms
GO:0005622 intracellular
GO:0005097 Rab GTPase activator activity
GO:0032313 regulation of Rab GTPase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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