C-Reactive Protein antibody
Sheep anti Human C-Reactive Protein antibody detects human C-reactive protein (CRP), a calcium-binding plasma protein which is significantly up-regulated during the acute phase response. CRP has been shown to bind phosphocholine residues, apoptotic cells and constituents of micro-organisms, and can lead to activation of leukocytes and the complement system, and the clearance of apoptotic cells.
CRP is produced in the liver in response to interleukin-1 (IL-1) and IL-6 secretion, and has a monomer molar mass of 25 kDa, and native cyclic pentamer mass of 125 kDa.
CRP is produced in the liver in response to interleukin-1 (IL-1) and IL-6 secretion, and has a monomer molar mass of 25 kDa, and native cyclic pentamer mass of 125 kDa.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CRP were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native c-reactive protein, from serum
- Approx. Protein Concentrations
- IgG concentration 5.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02741
- Entrez Gene
- CRP
- GO Terms
- GO:0005515 protein binding
- GO:0006953 acute-phase response
- GO:0046872 metal ion binding
- GO:0008228 opsonization
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010888 negative regulation of lipid storage
- GO:0030169 low-density lipoprotein particle binding
- GO:0033265 choline binding
- GO:0051637 Gram-positive bacterial cell surface binding
- Regulatory
- For research purposes only
Applications of C-Reactive Protein antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
Further Reading
-
DeFerranti, S.D. & Rifai, N. (2007) C-reactive protein: a nontraditional serum marker of cardiovascular risk.
Cardiovasc Pathol. 16 (1): 14-21. -
Yeh, E.T. (2004) CRP as a mediator of disease.
Circulation. 109 (21 Suppl 1): II11-4.
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