Beclin-1 antibody
Rabbit anti Beclin-1 (N-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Beclin-1
- Region
- (N-TERMINAL)
Rabbit anti Human Beclin-1 antibody recognizes an epitope within the N-terminal region of Beclin-1, a ~52 kDa coiled-coil protein which shares structural homology with the yeast autophagy gene apg6/vps30. The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Beclin-1 is a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumor suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human Beclin-1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- A 17 amino acid peptide located near human Beclin-1 amino-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.5ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Western Blotting
- AHP1009 detects a band of approximately 52kDa in human 293 cell lysates.
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 | ||||||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
Further Reading
-
Liang, X.H. et al. (1999) Induction of autophagy and inhibition of tumorigenesis by beclin 1.
Nature. 402 (6762): 672-6. -
Gozuacik, D. & Kimchi, A. (2004) Autophagy as a cell death and tumor suppressor mechanism.
Oncogene. 23 (16): 2891-906. -
Liang, X.H. et al. (1998) Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.
J Virol. 72 (11): 8586-96.
- RRID
- AB_609563
- UniProt
- Q14457
- O88597
- Q91XJ1
- Entrez Gene
- Becn1
- BECN1
- Becn1
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0009615 response to virus
- GO:0005802 trans-Golgi network
- GO:0006914 autophagy
- GO:0008285 negative regulation of cell proliferation
- GO:0016023 cytoplasmic membrane-bounded vesicle
- GO:0016239 positive regulation of macroautophagy
- GO:0043234 protein complex
- View More GO Terms
- GO:0006916 anti-apoptosis
- GO:0006968 cellular defense response
- GO:0000045 autophagic vacuole assembly
AHP1009
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