Beclin-1 antibody

Rabbit anti Beclin-1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Beclin-1
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1009
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F P * WB 0.1 mg loader
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loader
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Rabbit anti Human Beclin-1 antibody recognizes an epitope within the N-terminal region of Beclin-1, a ~52 kDa coiled-coil protein which shares structural homology with the yeast autophagy gene apg6/vps30.

The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Beclin-1 is a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumor suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human Beclin-1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 17 amino acid peptide located near human Beclin-1 amino-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Paraffin 1
Western Blotting 0.5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1009 detects a band of approximately 52kDa in human 293 cell lysates.

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Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Liang, X.H. et al. (1999) Induction of autophagy and inhibition of tumorigenesis by beclin 1.
    Nature. 402 (6762): 672-6.
  2. Gozuacik, D. & Kimchi, A. (2004) Autophagy as a cell death and tumor suppressor mechanism.
    Oncogene. 23 (16): 2891-906.
  3. Liang, X.H. et al. (1998) Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.
    J Virol. 72 (11): 8586-96.

RRID
AB_609563
UniProt
Q14457
O88597
Q91XJ1
Entrez Gene
Becn1
BECN1
Becn1
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0009615 response to virus
GO:0005802 trans-Golgi network
GO:0006914 autophagy
GO:0008285 negative regulation of cell proliferation
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0016239 positive regulation of macroautophagy
GO:0043234 protein complex
GO:0006916 anti-apoptosis
GO:0006968 cellular defense response
GO:0000045 autophagic vacuole assembly
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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