Bcl-xL antibody
Rabbit anti Human Bcl-xL antibody recognizes the long isoform of human Bcl-2-like protein 1, otherwise known as Bcl-xL, located on the mitochondrial membrane. The Bcl-xL isoform (the longer isoform) is a potent inhibitor of apoptosis. It appears to regulate cell death by binding to and blocking the voltage-dependent anion channel, thereby preventing the release of the caspase activator cytochrome c. High expression of Bcl-xL in tumours correlates to advanced disease and a poor prognosis.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Bcl-xL was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to 14 amino acids near the carboxy terminus of human Bcl-xL precursor.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q07817
- Entrez Gene
- BCL2L1
- GO Terms
- GO:0001836 release of cytochrome c from mitochondria
- GO:0016021 integral to membrane
- GO:0005741 mitochondrial outer membrane
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008634 negative regulation of survival gene product expression
- GO:0042802 identical protein binding
- GO:0031965 nuclear membrane
- GO:0034097 response to cytokine stimulus
- GO:0046902 regulation of mitochondrial membrane permeability
- GO:0051881 regulation of mitochondrial membrane potential
- Regulatory
- For research purposes only
Applications of Bcl-xL antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1 | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1722 detects a band of approximately 27kDa in A549 cell lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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