ATG7 antibody

100% Secure


Rabbit anti Human ATG7 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1651T C WB 50 µg
AHP1651 C WB 0.1 mg
Rabbit anti Human ATG7 (C-Terminal) antibody recognizes human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7 and APG7, a ~78 kDa cytoplasmic protein.

In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Studies indicate that caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death.

ATG7 is evolutionally conserved and exhibits high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to an 17 amino acid sequence from near the carboxy terminus of human ATG7.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O95352 Related reagents
Q9D906 Related reagents
Entrez Gene
ATG7 Related reagents
Atg7 Related reagents
GO Terms
GO:0004839 ubiquitin activating enzyme activity
GO:0005737 cytoplasm
GO:0006497 protein lipidation
GO:0006914 autophagy
GO:0006944 cellular membrane fusion
GO:0015031 protein transport
GO:0019778 APG12 activating enzyme activity
GO:0031401 positive regulation of protein modification process
GO:0042803 protein homodimerization activity
GO:0005515 protein binding
GO:0001889 liver development
GO:0006520 cellular amino acid metabolic process
GO:0006996 organelle organization
GO:0050877 neurological system process
GO:0007628 adult walking behavior
GO:0009791 post-embryonic development
GO:0016044 cellular membrane organization
GO:0021680 cerebellar Purkinje cell layer development
GO:0021860 pyramidal neuron development
GO:0021955 central nervous system neuron axonogenesis
GO:0021987 cerebral cortex development
GO:0030163 protein catabolic process
GO:0031396 regulation of protein ubiquitination
GO:0032446 protein modification by small protein conjugation
GO:0042594 response to starvation
GO:0043066 negative regulation of apoptosis
GO:0055013 cardiac muscle cell development
Regulatory
For research purposes only

Applications of ATG7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1651 detects a band of approximately 78kDa in Caco-2 cell lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunofluorescence

Product Specific References

References for ATG7 antibody

  1. Pampliega, O. et al. (2013) Functional interaction between autophagy and ciliogenesis.
    Nature. 502 (7470): 194-200.