ATG7 antibody

Rabbit anti Human ATG7 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ATG7
Region
(C-Terminal)

Product Code Applications Pack Size List Price Your Price Qty
AHP1651
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C WB 0.1 mg loader
List Price Your Price
loader
AHP1651T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C WB 50 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Human ATG7 (C-Terminal) antibody recognizes human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7, and APG7. ATG7 is a ~78 kDa cytoplasmic protein.

In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, and starvation-induced bulk degradation of proteins and organelles. Caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death (Yu et al. 2004).

ATG7 is evolutionarily conserved exhibiting high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.02% Sodium Azide (NaN3)
Pack Size: 50 µg
<0.1% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to an 17 amino acid sequence from near the carboxy terminus of human ATG7.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1651 detects a band of approximately 78kDa in Caco-2 cell lysate.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for ATG7 antibody

  1. Pampliega, O. et al. (2013) Functional interaction between autophagy and ciliogenesis.
    Nature. 502 (7470): 194-200.
  2. Koerver, L. et al. (2019) The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage.
    EMBO Rep. 20 (10): e48014.

Immunofluorescence

Synonyms
UBIQUITIN-ACTIVATING Enzyme E1-LIKE Protein
RRID
AB_1898218
UniProt
O95352
Q9D906
Entrez Gene
ATG7
Atg7
GO Terms
GO:0004839 ubiquitin activating enzyme activity
GO:0005737 cytoplasm
GO:0006497 protein lipidation
GO:0006914 autophagy
GO:0006944 cellular membrane fusion
GO:0015031 protein transport
GO:0019778 APG12 activating enzyme activity
GO:0031401 positive regulation of protein modification process
GO:0042803 protein homodimerization activity
GO:0005515 protein binding
GO:0001889 liver development
GO:0006520 cellular amino acid metabolic process
GO:0006996 organelle organization
GO:0050877 neurological system process
GO:0007628 adult walking behavior
GO:0009791 post-embryonic development
GO:0016044 cellular membrane organization
GO:0021680 cerebellar Purkinje cell layer development
GO:0021860 pyramidal neuron development
GO:0021955 central nervous system neuron axonogenesis
GO:0021987 cerebral cortex development
GO:0030163 protein catabolic process
GO:0031396 regulation of protein ubiquitination
GO:0032446 protein modification by small protein conjugation
GO:0042594 response to starvation
GO:0043066 negative regulation of apoptosis
GO:0055013 cardiac muscle cell development
View more products with ATG7 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products