ATG3 antibody

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Rabbit anti ATG3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2219 IP WB 0.1 mg
Mouse anti Human ATG3 antibody recognises ATG3 (Autophagy-related protein 3), otherwise known as APG3, a widely expressed E2-like enzyme, and member of the ATG3 family, which plays a key role in autophagosome formation, by catalyzing the conjugation of ATG8-like proteins to phosphatidylethanolamine (PE), in conjunction with ATG7.

ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.

Mouse anti Human ATG3 antibody (AHP2219) has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ATG3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 17-32 of human ATG3. The corresponding sequence is identical in rat and mouse.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9NT62 Related reagents
Q9CPX6 Related reagents
Q6AZ50 Related reagents
Entrez Gene
Atg3 Related reagents
Atg3 Related reagents
ATG3 Related reagents
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005829 cytosol
GO:0015031 protein transport
GO:0019776 Atg8 ligase activity
GO:0019777 Atg12 ligase activity
GO:0043653 mitochondrial fragmentation involved in apoptosis
GO:0000153 cytoplasmic ubiquitin ligase complex
GO:0016567 protein ubiquitination
GO:0006612 protein targeting to membrane
GO:0019899 enzyme binding
Regulatory
For research purposes only

Applications of ATG3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 5 10ul
Western Blotting 2.0 4.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2219 detects a band of approximately 36kDa in HeLa and mouse 3T3 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*