ATG3 antibody
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Mouse anti Human ATG3 antibody recognises ATG3 (Autophagy-related protein 3), otherwise known as APG3, a widely expressed E2-like enzyme, and member of the ATG3 family, which plays a key role in autophagosome formation, by catalyzing the conjugation of ATG8-like proteins to phosphatidylethanolamine (PE), in conjunction with ATG7.
ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.
Mouse anti Human ATG3 antibody (AHP2219) has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.
ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.
Mouse anti Human ATG3 antibody (AHP2219) has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human ATG3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 17-32 of human ATG3. The corresponding sequence is identical in rat and mouse.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q9NT62 Related reagents
- Q9CPX6 Related reagents
- Q6AZ50 Related reagents
- Entrez Gene
- Atg3 Related reagents
- Atg3 Related reagents
- ATG3 Related reagents
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005829 cytosol
- GO:0015031 protein transport
- GO:0019776 Atg8 ligase activity
- GO:0019777 Atg12 ligase activity
- GO:0043653 mitochondrial fragmentation involved in apoptosis
- GO:0000153 cytoplasmic ubiquitin ligase complex
- GO:0016567 protein ubiquitination
- GO:0006612 protein targeting to membrane
- GO:0019899 enzyme binding
- Regulatory
- For research purposes only
Applications of ATG3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | 5 | 10ul | |
| Western Blotting | 2.0 | 4.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2219 detects a band of approximately 36kDa in HeLa and mouse 3T3 cell lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
