ATG3 antibody

ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.
Mouse anti Human ATG3 antibody (AHP2219) has been shown to be suitable for use in immunoprecipitation using rat NRK cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human ATG3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 17-32 of human ATG3. The corresponding sequence is identical in rat and mouse.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NT62
- Q9CPX6
- Q6AZ50
- Entrez Gene
- Atg3
- Atg3
- ATG3
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005829 cytosol
- GO:0015031 protein transport
- GO:0019776 Atg8 ligase activity
- GO:0019777 Atg12 ligase activity
- GO:0043653 mitochondrial fragmentation involved in apoptosis
- GO:0000153 cytoplasmic ubiquitin ligase complex
- GO:0016567 protein ubiquitination
- GO:0006612 protein targeting to membrane
- GO:0019899 enzyme binding
- Regulatory
- For research purposes only
Applications of ATG3 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunoprecipitation | 5 | 10ul | |
Western Blotting | 2.0 | 4.0ug/ml |
- Western Blotting
- AHP2219 detects a band of approximately 36kDa in HeLa and mouse 3T3 cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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