Annexin II antibody

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Rabbit anti Annexin II

Annexin II antibody recognizes annexin A2, also known as annexin II, annexin-2, calpactin I heavy chain, calpactin-1 heavy chain, chromobindin-8, lipocortin II, placental anticoagulant protein IV, or p36. The antibody has been extensively validated for western blotting using whole cell lysates.

Annexin II Antibody with Control Lysate

Annexin II antibody recognizes annexin A2, also known as annexin II, annexin-2, calpactin I heavy chain, calpactin-1 heavy chain, chromobindin-8, lipocortin II, placental anticoagulant protein IV, PAP-IV, protein I or p36. The antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00011 WB 100 µl
VPA00011KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human annexin II antibody recognizes human annexin II also known as annexin A2 (ANXA2), lipocortin 2, chromobindin-8 or placental anticoagulant protein IV. Annexin II is a 339 amino acid secreted protein containing 4 annexin repeats. Annexin II is a pleiotropic calcium-dependent phospholipid-binding protein and member of the annexin family, abundantly expressed in a variety of tissues including the brain, spleen, kidney and placenta. The protein can exist in a monomer, heterodimer or heterotetramer form.

Annexin II acts as an autocrine factor, enhancing the formation of osteoclasts and resorption of bone, and is implicated in both exocytosis and endocytosis. Annexin II is a substrate for both tyrosine and serine/threonine kinases, and is phosphorylated following growth factor stimulation and cellular activation by viral transformation (Brambilla et al. 1991).

Rabbit anti Human annexin II antibody recognizes annexin II as a single band of ~39 kDa in A549 cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell line lysates tested.

Product Details

Target Species
Human
Western Blotting
Anti annexin II antibody detects a band of approximately 39 kDa in A549 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
6x His-tagged full-length recombinant human ANXA2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P07355 Related reagents
Entrez Gene
ANXA2 Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0005509 calcium ion binding
GO:0004859 phospholipase inhibitor activity
GO:0005544 calcium-dependent phospholipid binding
GO:0005604 basement membrane
GO:0005625 soluble fraction
GO:0008092 cytoskeletal protein binding
GO:0030496 midbody
GO:0031340 positive regulation of vesicle fusion
GO:0042470 melanosome
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Annexin II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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