Annexin I antibody

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Rabbit anti Annexin I

Annexin I antibody recognizes annexin A1, also known as annexin I, annexin-1, calpactin I, calpactin-2, chromobindin-9, lipocortin I, phospholipase A2 inhibitory protein or p35. The antibody has been extensively validated for western blotting using whole cell lysates.

Annexin I Antibody with Control Lysate

Annexin I antibody recognizes annexin A1, also known as annexin I, annexin-1, calpactin I, calpactin-2, chromobindin-9, lipocortin I, phospholipase A2 inhibitory protein or p35. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00078 WB 100 µl
VPA00078KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human annexin I antibody recognizes human annexin I (ANXA1), otherwise known as lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family.

Annexin is expressed on the cytosolic face of the plasma membrane, acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity. Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive diagnostic marker.

Rabbit anti Human annexin I antibody detects asingle band of 39 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti annexin I detects a band of approximately 39 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human ANXA1 full-length recombinant protein 6x His tagged.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P04083 Related reagents
Entrez Gene
ANXA1 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005102 receptor binding
GO:0005576 extracellular region
GO:0005198 structural molecule activity
GO:0005544 calcium-dependent phospholipid binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005929 cilium
GO:0006629 lipid metabolic process
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0016323 basolateral plasma membrane
GO:0018149 peptide cross-linking
GO:0019834 phospholipase A2 inhibitor activity
GO:0030216 keratinocyte differentiation
GO:0031340 positive regulation of vesicle fusion
GO:0046632 alpha-beta T cell differentiation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Annexin I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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