Annexin I antibody
Rabbit anti Annexin I
Annexin I antibody recognizes annexin A1, also known as annexin I, annexin-1, calpactin I, calpactin-2, chromobindin-9, lipocortin I, phospholipase A2 inhibitory protein or p35. The antibody has been extensively validated for western blotting using whole cell lysates.
Annexin I Antibody with Control Lysate
Annexin I antibody recognizes annexin A1, also known as annexin I, annexin-1, calpactin I, calpactin-2, chromobindin-9, lipocortin I, phospholipase A2 inhibitory protein or p35. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00078 | WB | 100 µl | ||||
| VPA00078KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human annexin I antibody recognizes human annexin I (ANXA1), otherwise known as lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family.
Annexin is expressed on the cytosolic face of the plasma membrane, acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity. Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive diagnostic marker.
Rabbit anti Human annexin I antibody detects asingle band of 39 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Annexin is expressed on the cytosolic face of the plasma membrane, acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity. Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive diagnostic marker.
Rabbit anti Human annexin I antibody detects asingle band of 39 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti annexin I detects a band of approximately 39 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human ANXA1 full-length recombinant protein 6x His tagged.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04083 Related reagents
- Entrez Gene
- ANXA1 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0001533 cornified envelope
- GO:0030674 protein binding, bridging
- GO:0005102 receptor binding
- GO:0005576 extracellular region
- GO:0005198 structural molecule activity
- GO:0005544 calcium-dependent phospholipid binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005929 cilium
- GO:0006629 lipid metabolic process
- GO:0006916 anti-apoptosis
- GO:0006928 cellular component movement
- GO:0006954 inflammatory response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0016323 basolateral plasma membrane
- GO:0018149 peptide cross-linking
- GO:0019834 phospholipase A2 inhibitor activity
- GO:0030216 keratinocyte differentiation
- GO:0031340 positive regulation of vesicle fusion
- GO:0046632 alpha-beta T cell differentiation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Annexin I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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