Annexin I antibody

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Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 4

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with annexin I antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/50,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to annexin I (molecular weight 39 kDa)

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of Annexin I knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti Annexin I antibody (**VPA00078**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 141 second and (B) 30 second exposure.

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of Annexin I from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 4 — Paraffin embedded human colon carcinoma stained with Rabbit anti human Annexin I (**VPA00078**).

Rabbit anti Annexin I

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00078 Datasheet	E P WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human annexin I antibody recognizes human annexin I (ANXA1), otherwise known as lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family.

Annexin is expressed on the cytosolic face of the plasma membrane, acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity. Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive diagnostic marker.

Rabbit anti Human annexin I antibody detects asingle band of 39 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species

Human

Western Blotting

Rabbit anti annexin I detects a band of approximately 39 kDa in K562 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Human ANXA1 full-length recombinant protein 6x His tagged.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: P04083
Entrez Gene: ANXA1
GO Terms: GO:0005509 calcium ion binding; GO:0001533 cornified envelope; GO:0030674 protein binding, bridging; GO:0005102 receptor binding; GO:0005576 extracellular region; GO:0005198 structural molecule activity; GO:0005544 calcium-dependent phospholipid binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005929 cilium; GO:0006629 lipid metabolic process; GO:0006916 anti-apoptosis; GO:0006928 cellular component movement; GO:0006954 inflammatory response; GO:0007166 cell surface receptor linked signaling pathway; GO:0016323 basolateral plasma membrane; GO:0018149 peptide cross-linking; GO:0019834 phospholipase A2 inhibitor activity; GO:0030216 keratinocyte differentiation; GO:0031340 positive regulation of vesicle fusion; GO:0046632 alpha-beta T cell differentiation
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Annexin I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Paraffin
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

Product Specific References

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