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Annexin I antibody

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Western Blot Validated
Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with annexin I antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/50,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to annexin I (molecular weight 39 kDa)
Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of Annexin I knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti Annexin I antibody (VPA00078) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 141 second and (B) 30 second exposure.
Anti Annexin I Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of Annexin I from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti Annexin I

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00078
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human annexin I antibody recognizes human annexin I (ANXA1), otherwise known as lipocortin I, a calcium-dependent phospholipid-binding protein and member of the annexin family.

Annexin is expressed on the cytosolic face of the plasma membrane, acts as an inhibitor of phospholipase A2, and may exhibit anti-inflammatory activity. Increased expression of the ANXA1 gene in hairy cell leukaemia, can be used in immunohistochemistry as a highly-sensitive diagnostic marker.

Rabbit anti Human annexin I antibody detects asingle band of 39 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti annexin I detects a band of approximately 39 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human ANXA1 full-length recombinant protein 6x His tagged.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P04083
Entrez Gene
ANXA1
GO Terms
GO:0005509 calcium ion binding
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005102 receptor binding
GO:0005576 extracellular region
GO:0005198 structural molecule activity
GO:0005544 calcium-dependent phospholipid binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005929 cilium
GO:0006629 lipid metabolic process
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0016323 basolateral plasma membrane
GO:0018149 peptide cross-linking
GO:0019834 phospholipase A2 inhibitor activity
GO:0030216 keratinocyte differentiation
GO:0031340 positive regulation of vesicle fusion
GO:0046632 alpha-beta T cell differentiation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Annexin I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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