Amylase antibody

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Sheep anti Human Amylase (Salivary):HRP

Sheep anti Human Amylase (Salivary)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0480-0404P C E 1 ml
0480-0404 C E 1 ml
Sheep anti Human amylase (salivary) antibody recognizes salivary amylase, formally known as ptyalin, an alpha-amylase digestive enzyme and member of the glycosyl hydrolase 13 family, which breaks down insoluble starch into soluble starches (dextrins) and maltose.

Sheep anti Human Amylase (Salivary) antibody also recognizes porcine amylase (50%) and weakly fungal (Aspergillus spp.) amylase (10%).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Fungal
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human salivary amylase was raised by repeated immunisation of sheep with highly purified antigen.
Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Highly pure human salivary amylase.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04745 Related reagents
Entrez Gene
AMY1C Related reagents
GO Terms
GO:0005515 protein binding
GO:0004556 alpha-amylase activity
GO:0005576 extracellular region
GO:0046872 metal ion binding
GO:0005975 carbohydrate metabolic process
GO:0007586 digestion
Regulatory
For research purposes only

Applications of Amylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/500
Immunohistology - Frozen
ELISA 1/500 1/3000
Immunohistology - Frozen 1/20 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for Amylase antibody

  1. Bachman, D.E. et al. (2015) Prospective diagnostic accuracy evaluation and clinical utilization of a modified assay for platelet-associated immunoglobulin in thrombocytopenic and nonthrombocytopenic dogs.
    Vet Clin Pathol. Aug 24. [Epub ahead of print]