Amylase antibody

Sheep anti Human Amylase (Salivary)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Amylase
Region
(SALIVARY)

Product Code Applications Pack Size List Price Your Price Qty
0480-0404
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 1 ml
List Price Your Price
Search for Batch Specific Datasheets

Sheep anti Human amylase (salivary) antibody recognizes salivary amylase, formally known as ptyalin, an alpha-amylase digestive enzyme and member of the glycosyl hydrolase 13 family, which breaks down insoluble starch into soluble starches (dextrins) and maltose.

Sheep anti Human Amylase (Salivary) antibody also recognizes porcine amylase (50%) and weakly fungal (Aspergillus spp.) amylase (10%).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Fungal Expected from Sequence
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human salivary amylase was raised by repeated immunisation of sheep with highly purified antigen.
Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Highly pure human salivary amylase.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/3000
Immunohistology - Frozen 1/20 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Amylase antibody

  1. Bachman, D.E. et al. (2015) Prospective diagnostic accuracy evaluation and clinical utilization of a modified assay for platelet-associated immunoglobulin in thrombocytopenic and nonthrombocytopenic dogs.
    Vet Clin Pathol. Aug 24. [Epub ahead of print]

RRID
AB_620383
UniProt
P04745
Entrez Gene
AMY1C
GO Terms
GO:0005515 protein binding
GO:0004556 alpha-amylase activity
GO:0005576 extracellular region
GO:0046872 metal ion binding
GO:0005975 carbohydrate metabolic process
GO:0007586 digestion
View more products with Amylase specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products