Alpha 2 Macroglobulin antibody

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Sheep anti Human Alpha 2 Macroglobulin:HRP

Sheep anti Human Alpha 2 Macroglobulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5850-0004P C E 1 ml
5850-0004 C E 1 ml
Sheep anti Human alpha 2 macroglobulin antibody recognizes human alpha-2-macroglobulin, a secreted protease inhibitor known to suppress the function of all four classes of proteinases. Sheep anti Human alpha 2 macroglobulin antibody exhibits minimal cross reactivity with related serum proteins.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified human alpha-2 macroglobulin
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01023 Related reagents
Entrez Gene
A2M Related reagents
GO Terms
GO:0001869 negative regulation of complement activation, lectin pathway
GO:0002576 platelet degranulation
GO:0005829 cytosol
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005096 GTPase activator activity
GO:0005615 extracellular space
GO:0007264 small GTPase mediated signal transduction
GO:0007597 blood coagulation, intrinsic pathway
GO:0019899 enzyme binding
GO:0019959 interleukin-8 binding
GO:0019966 interleukin-1 binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043120 tumor necrosis factor binding
GO:0051056 regulation of small GTPase mediated signal transduction
Regulatory
For research purposes only

Applications of Alpha 2 Macroglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/500
Immunohistology - Frozen 1/50 1/500
ELISA
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Alpha 2 Macroglobulin antibody

  1. Nedić, O. et al. (2017) Analysis of Four Circulating Complexes of Insulin-Like Growth Factor Binding Proteins in Human Blood during Aging.
    Biochemistry (Mosc). 82 (10): 1200-6.