Alpha 1 Microglobulin antibody

100% Secure


Sheep anti Human Alpha 1 Microglobulin:HRP

Sheep anti Human Alpha 1 Microglobulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
6220-0004P C E 1 ml
6220-0004G E ID 1 ml
Sheep anti Human alpha 1 microglobulin antibody recognizes human alpha 1 microglobulin. Sheep anti Human alpha 1 microglobulin antibody does not demonstrate any cross reactivity with urine albumin, retinol binding protein or beta-2 microglobulin in performed testing.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Antiserum Preparation
Antisera to human alpha-1-microglubulin were raised by repeated immunisations of sheep with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)>
Immunogen
Highly pure human alpha-1-microglobulin, prepared from the urine of patients with chronic renal proteinuria, molecular weight 25-30 kDa.
Approx. Protein Concentrations
IgG concentration 1mg/ml
Approx. Protein Concentrations
5mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02760 Related reagents
Entrez Gene
AMBP Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005215 transporter activity
GO:0007565 female pregnancy
GO:0018298 protein-chromophore linkage
GO:0019855 calcium channel inhibitor activity
GO:0019862 IgA binding
GO:0020037 heme binding
GO:0042167 heme catabolic process
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
GO:0046329 negative regulation of JNK cascade
GO:0046904 calcium oxalate binding
GO:0050777 negative regulation of immune response
Regulatory
For research purposes only

Applications of Alpha 1 Microglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/300
Immunohistology - Frozen 1/10 1/500
ELISA 1/500 1/1000
Immunodiffusion
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Excessive excretion of this protein has been known to indicate abnormalities of the renal tubules.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E