Actin Beta antibody
Rabbit anti actin beta antibody (AHP2417 used as a loading control in western blot analysis of preadipocyte cell lysates treated with agents affecting adipogenesis.
Image caption:
Z. jujuba leaf extract (ZJL), apigenin (APG), betulinic acid (BA) and maslinic acid (MA) inhibited adipogenesis through a modulation of major adipogenic regulators. Representative images of the Western blots
From: Savova MS, Vasileva LV, Mladenova SG, Amirova KM, Ferrante C, Orlando G, Wabitsch M, Georgiev MI.
Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.
Biomed Pharmacother. 2021 Sep;141:111934.
doi: 10.1016/j.biopha.2021.111934.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Actin Beta
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Actin Beta
Antibody Quality and Performance Guarantee
| Rabbit anti actin beta antibody recognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling. Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antiserum to actin beta was raised by repeated immunisation of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Peptide mapping to the terminal domain of human actin beta
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti actin beta recognizes a band of appriximately 42 kDa when run under reducing conditions
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for Actin Beta antibody
-
Chen, X.J. et al. (2018) Association of reduced sclerostin expression with collapse process in patients with osteonecrosis of the femoral head.
Int Orthop. 42 (7): 1675-82. -
Savova, M.S. et al. (2021) Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.
Biomed Pharmacother. 141: 111934. -
ElSayed, M.H. et al. (2023) Betanin improves motor function and alleviates experimental Parkinsonism via downregulation of TLR4/MyD88/NF-κB pathway: Molecular docking and biological investigations.
Biomed Pharmacother. 164: 114917.
AHP2417
151835
154062
163093
166690
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