Actin Beta antibody
Rabbit anti actin beta antibody (AHP2417 used as a loading control in western blot analysis of preadipocyte cell lysates treated with agents affecting adipogenesis.
Image caption:
Z. jujuba leaf extract (ZJL), apigenin (APG), betulinic acid (BA) and maslinic acid (MA) inhibited adipogenesis through a modulation of major adipogenic regulators. Representative images of the Western blots
From: Savova MS, Vasileva LV, Mladenova SG, Amirova KM, Ferrante C, Orlando G, Wabitsch M, Georgiev MI.
Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.
Biomed Pharmacother. 2021 Sep;141:111934.
doi: 10.1016/j.biopha.2021.111934.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti actin beta antibody recognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.
Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.
Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antiserum to actin beta was raised by repeated immunisation of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Peptide mapping to the terminal domain of human actin beta
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of Actin Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti actin beta recognizes a band of appriximately 42 kDa when run under reducing conditions
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Actin Beta antibody
-
Chen, X.J. et al. (2018) Association of reduced sclerostin expression with collapse process in patients with osteonecrosis of the femoral head.
Int Orthop. 42 (7): 1675-82. -
Savova, M.S. et al. (2021) Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.
Biomed Pharmacother. 141: 111934.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
