4EBP1 antibody

Rabbit anti 4EBP1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
4EBP1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP993
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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loader
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Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.

The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.

Rabbit anti 4EBP1 antibody (AHP993) has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen.

Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 14 amino acid peptide located near human 4EBP1 carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 2.5 5.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP993 detects a band of approximately 20kDa in mouse 3T3 cell lysates. (predicted Mwt. 13kDa).

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Further Reading

  1. Hara, K. et al. (1997) Regulation of eIF-4E BP1 phosphorylation by mTOR.
    J Biol Chem. 272 (42): 26457-63.
  2. Haghighat, A. et al. (1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.
    EMBO J. 14 (22): 5701-9.

Synonyms
eIF-4EBP1
RRID
AB_2097985
UniProt
Q13541
Entrez Gene
EIF4EBP1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005829 cytosol
GO:0006412 translation
GO:0008286 insulin receptor signaling pathway
GO:0031929 TOR signaling cascade
GO:0045931 positive regulation of mitotic cell cycle
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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