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4EBP1 antibody

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Anti 4EBP1 (C-Terminal) Antibody thumbnail image 1 Anti 4EBP1 (C-Terminal) Antibody thumbnail image 2 Anti 4EBP1 (C-Terminal) Antibody thumbnail image 3
Anti 4EBP1 (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysates from 3T3 mouse embryonic fibroblast cells probed with Rabbit anti Human 4E-BP1 (AHP993) at 2.5(A), 5(B) and 10(C) μg/ml.
Anti 4EBP1 (C-Terminal) Antibody gallery image 2
Immunocytochemical stainig of 3T3 cells with Rabbit anti Human 4E-BP1 (AHP993).
Anti 4EBP1 (C-Terminal) Antibody gallery image 3
Western blot analysis of HepG2 whole cell lysates probed with Rabbit anti 4EBP1 (AHP993) at 1/1000 dilution with 60 second Clarity Max exposure. Arrow points to 4EBP1.

Rabbit anti 4EBP1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP993
Datasheet
WB
SDS
0.1 mg loader

Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.

The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.

Rabbit anti 4EBP1 antibody (AHP993) has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen.

Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 14 amino acid peptide located near human 4EBP1 carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q13541
Entrez Gene
EIF4EBP1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005829 cytosol
GO:0006412 translation
GO:0008286 insulin receptor signaling pathway
GO:0031929 TOR signaling cascade
GO:0045931 positive regulation of mitotic cell cycle
Regulatory
For research purposes only

Applications of 4EBP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 2.5 5.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP993 detects a band of approximately 20kDa in mouse 3T3 cell lysates. (predicted Mwt. 13kDa).
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Hara, K. et al. (1997) Regulation of eIF-4E BP1 phosphorylation by mTOR.
    J Biol Chem. 272 (42): 26457-63.
  2. Haghighat, A. et al. (1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.
    EMBO J. 14 (22): 5701-9.

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