4EBP1 antibody
Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.
The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.
Rabbit anti 4EBP1 antibody (AHP993) has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.
The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.
Rabbit anti 4EBP1 antibody (AHP993) has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A 14 amino acid peptide located near human 4EBP1 carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q13541 Related reagents
- Entrez Gene
- EIF4EBP1 Related reagents
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0005829 cytosol
- GO:0006412 translation
- GO:0008286 insulin receptor signaling pathway
- GO:0031929 TOR signaling cascade
- GO:0045931 positive regulation of mitotic cell cycle
- Regulatory
- For research purposes only
Applications of 4EBP1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 2.5 | 5.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP993 detects a band of approximately 20kDa in mouse 3T3 cell lysates. (predicted Mwt. 13kDa).
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
|
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Hara, K. et al. (1997) Regulation of eIF-4E BP1 phosphorylation by mTOR.
J Biol Chem. 272 (42): 26457-63. -
Haghighat, A. et al. (1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.
EMBO J. 14 (22): 5701-9.
