IgG antibody

Goat anti Hamster IgG:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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STAR79B
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Goat anti Hamster IgG antibody recognizes Golden Syrian and Armenian hamster IgG (H+L) and has been adsorbed against both mouse and rat immunoglobulins to minimise cross-reactivity.

Target Species
Hamster
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to hamster IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Hamster IgG.
Approx. Protein Concentrations
IgG concentration 0.8 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/5000
Flow Cytometry 1/25 1/50
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG antibody

  1. Samant, M. et al. (2009) Immunization with the DNA-encoding N-terminal domain of proteophosphoglycan of Leishmania donovani generates Th1-type immunoprotective response against experimental visceral leishmaniasis.
    J Immunol. 183: 470-9.
  2. Moore, G.T. et al. (2008) Glycosylation changes in hFUT1 transgenic mice increase TCR signaling and apoptosis resulting in thymocyte maturation arrest.
    Mol Immunol. 45: 2401-10.
  3. Forster, K.M. et al. (2015) DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis.
    Mem Inst Oswaldo Cruz. 110 (8): 989-95.
  4. Verma R et al. (2015) Cross reactive molecules of human lymphatic filaria Brugia malayi inhibit Leishmania donovani infection in hamsters.
    Acta Trop. 152: 103-11.
  5. Wiśniewski, M. et al. (2016) Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection.
    Exp Parasitol. 163: 1-7.
  6. Bacelo, K.L. et al. (2014) Xanthan gum as an adjuvant in a subunit vaccine preparation against leptospirosis.
    Biomed Res Int. 2014: 636491.
  7. Verma, R. et al. (2018) Leishmania donovani. molecules recognized by sera of filaria infected host facilitate filarial infection.
    Parasitol Res. 117 (9): 2901-12.
  8. Spitzova, T. et al. (2020) Interactions between host biogenic amines and sand fly salivary yellow-related proteins.
    Parasit Vectors. 13 (1): 237.
  9. View The Latest Product References

RRID
AB_322704
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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