GDNFR Alpha antibody
Rabbit anti GDNFα antibody recognizes GDNFR alpha, also known as GFR&alpha-1 (GFRα Nomenclature Committee 1997), GDNF receptor alpha-1, RET ligand 1 or TGF-beta-related neurotrophic factor receptor 1. GFR&alpha-1 is a 465 amino acid ~52 kDa GPI anchored cell surface receptor for glial cell derived neutrophic factor (GDNF).
GFR&alpha-1 is highly expressed in human gliomas (Wiesenhofer et al. 2000).
GFR&alpha-1 is highly expressed in human gliomas (Wiesenhofer et al. 2000).
Product Details
- Species Cross-Reactivity
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Target Species Cross Reactivity Rat Mouse Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human GDNFR alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Peptide from C terminus of human GDNFR alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P56159
- Entrez Gene
- GFRA1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005102 receptor binding
- GO:0007411 axon guidance
- GO:0031225 anchored to membrane
- GO:0016167 glial cell line-derived neurotrophic factor receptor activity
- GO:0019898 extrinsic to membrane
- Regulatory
- For research purposes only
Applications of GDNFR Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 1/500 | 1/1000 |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for GDNFR Alpha antibody
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Wiesenhofer, B. et al. (2000) Glial cell line-derived neurotrophic factor (GDNF) and its receptor (GFR-alpha 1) are strongly expressed in human gliomas.
Acta Neuropathol. 99: 131-7. -
Weis, C. et al. (2001) Nerve growth factor and glial cell line-derived neurotrophic factor restore the cholinergic neuronal phenotype in organotypic brain slices of the basal nucleus of Meynert.
Neuroscience. 102: 129-38.
Further Reading
-
Jing, S. et al. (1996) GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-alpha, a novel receptor for GDNF.
Cell. 85 (7): 1113-24. -
Treanor, J.J. et al. (1996) Characterization of a multicomponent receptor for GDNF.
Nature. 382 (6586): 80-3. -
(1997) Nomenclature of GPI-linked receptors for the GDNF ligand family. GFR(alpha) Nomenclature Committee.
Neuron 19: 485.
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