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GDNFR Alpha antibody

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Anti GDNFR Alpha Antibody thumbnail image 1 Anti GDNFR Alpha Antibody thumbnail image 2
Anti GDNFR Alpha Antibody gallery image 1
Western blot analysis of crude membrane fraction lysates from human brain (B), liver (L), kidney (K) and spleen(S) probed with Rabbit anti Human GDNFR alpha (AHP446).
Anti GDNFR Alpha Antibody gallery image 2
Immunohistochemical staining of human brain with Rabbit anti Human GDNFR alpha (AHP446).

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WB P

Rabbit anti GDNFR Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GDNFR Alpha

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Product Code Applications Pack Size List Price Your Price Qty
AHP446
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti GDNFα antibody recognizes GDNFR alpha, also known as GFRα-1 (GFRα Nomenclature Committee 1997), GDNF receptor alpha-1, RET ligand 1 or TGF-beta-related neurotrophic factor receptor 1. GFR&alpha-1 is a 465 amino acid ~52 kDa GPI anchored cell surface receptor for glial cell derived neutrophic factor (GDNF).

GFR&alpha-1 is highly expressed in human gliomas (Wiesenhofer et al. 2000).

Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human GDNFR alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide from C terminus of human GDNFR alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/500 1/1000
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for GDNFR Alpha antibody

  1. Wiesenhofer, B. et al. (2000) Glial cell line-derived neurotrophic factor (GDNF) and its receptor (GFR-alpha 1) are strongly expressed in human gliomas.
    Acta Neuropathol. 99: 131-7.
  2. Weis, C. et al. (2001) Nerve growth factor and glial cell line-derived neurotrophic factor restore the cholinergic neuronal phenotype in organotypic brain slices of the basal nucleus of Meynert.
    Neuroscience. 102: 129-38.
  3. Marco, S. et al. (2002) Excitatory amino acids differentially regulate the expression of GDNF, neurturin, and their receptors in the adult rat striatum.
    Exp Neurol. 174 (2): 243-52.

Further Reading

  1. Jing, S. et al. (1996) GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-alpha, a novel receptor for GDNF.
    Cell. 85 (7): 1113-24.
  2. Treanor, J.J. et al. (1996) Characterization of a multicomponent receptor for GDNF.
    Nature. 382 (6586): 80-3.
  3. (1997) Nomenclature of GPI-linked receptors for the GDNF ligand family. GFR(alpha) Nomenclature Committee.
    Neuron 19: 485.

RRID
AB_322749
UniProt
P56159
Entrez Gene
GFRA1
GO Terms
GO:0005886 plasma membrane
GO:0005102 receptor binding
GO:0007411 axon guidance
GO:0031225 anchored to membrane
GO:0016167 glial cell line-derived neurotrophic factor receptor activity
GO:0019898 extrinsic to membrane

AHP446

171002

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