GDNFR Alpha antibody

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Anti GDNFR Alpha Antibody thumbnail image 2

Anti GDNFR Alpha Antibody gallery image 1 — Western blot analysis of crude membrane fraction lysates from human brain (B), liver (L), kidney (K) and spleen(S) probed with Rabbit anti Human GDNFR alpha (**AHP446**).

Anti GDNFR Alpha Antibody gallery image 2 — Immunohistochemical staining of human brain with Rabbit anti Human GDNFR alpha (**AHP446**).

Rabbit anti GDNFR Alpha

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP446 Datasheet	P* WB MSDS	0.1 mg	Log in		Get Quote

Rabbit anti GDNFα antibody recognizes GDNFR alpha, also known as GFR&alpha-1 (GFRα Nomenclature Committee 1997), GDNF receptor alpha-1, RET ligand 1 or TGF-beta-related neurotrophic factor receptor 1. GFR&alpha-1 is a 465 amino acid ~52 kDa GPI anchored cell surface receptor for glial cell derived neutrophic factor (GDNF).

GFR&alpha-1 is highly expressed in human gliomas (Wiesenhofer et al. 2000).

Product Details

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Mouse
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human GDNFR alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide

Immunogen

Peptide from C terminus of human GDNFR alpha.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P56159 Related reagents
Entrez Gene: GFRA1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005102 receptor binding; GO:0007411 axon guidance; GO:0031225 anchored to membrane; GO:0016167 glial cell line-derived neurotrophic factor receptor activity; GO:0019898 extrinsic to membrane
Regulatory: For research purposes only

Applications of GDNFR Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹
Western Blotting		1/500	1/1000

¹ This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Sheep anti Rabbit IgG:Biotin	2AB02B	C E P WB	1 ml	Log in	Get Quote
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in	Get Quote
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote

Product Specific References

References for GDNFR Alpha antibody

Wiesenhofer, B. et al. (2000) Glial cell line-derived neurotrophic factor (GDNF) and its receptor (GFR-alpha 1) are strongly expressed in human gliomas.
Acta Neuropathol. 99: 131-7.
Weis, C. et al. (2001) Nerve growth factor and glial cell line-derived neurotrophic factor restore the cholinergic neuronal phenotype in organotypic brain slices of the basal nucleus of Meynert.
Neuroscience. 102: 129-38.