FITC antibody
HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS by ELISA
Image caption:
Comparison of LPS binding in indirect ELISA with different buffers and blocking agents.
FITC-LPS from E. coli serotype O111:B4 was incubated at 1.25 μg/ml in PBS-EDTA (PBS), with or without 0.05% Tween 20 (PBS-TW2) or Triton X-100 (PBS-TTX), in wells coated with polymyxin B (PMX) or lysozyme (LSZ) (1 μg/well), or empty wells (control), which were previously blocked with BSA (A) or OVA (B). Each assay was performed in triplicate. Mean values ± SD are shown in the graph.
From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016)
Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS ONE 11(6): e0156530.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS in a competitive hCAS ELISA
Image caption:
Competitive hCAS-ELISA performed under neutral conditions.
FITC-LPSs (5 μg/ml) from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated with different concentrations of proteins and peptides (40, 10, 1 and 0.25 μg/ml) in PBS-EDTA (pH 7.2) and added to the wells coated with casein hydrolysate (hCAS). Data are expressed as percentage inhibition of LPS binding to hCAS by the target molecule and are the mean values ±SD of duplicate wells. The average values of the optical density (492 nm) of control wells (without inhibitor) were 0.311 (±0.019, red dashed line) and 0.300 (±0.025, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. BSA, bovine serum albumin; HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B.
From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016)
Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS ONE 11(6): e0156530.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS in a competitive PMX ELISA
Image caption:
Competitive PMX-ELISA.
FITC-LPSs from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated, at 1.25 and 2.5 μg/ml, respectively, with three different concentrations of proteins and peptides (10, 1 and 0.25 μg/ml) in PBS-EDTA with 0.1% BSA and added to wells containing biotinylated polymyxin B captured by deglycosylated avidin. Results are expressed as percentage inhibition of LPS binding to captured polymyxin B by the target protein/peptide, and are the mean values ±SD for duplicate wells. The average optical densities (492 nm) of control wells (without inhibitor) were 0.904 (±0.042, red dashed line) and 0.842 (±0.036, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B.
From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016)
Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS ONE 11(6): e0156530.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Sheep anti FITC antibody (640005) used to detect bound FAM labeled peptides by ELISA
Image caption:
Synthesized peptides JB1, JB2, JB3, JB4, and JB5 bind to InlA. Wells of a microtiter plate were coated with 1 μg InlA blocking and incubated with increasing concentrations of FAM-labeled peptides. Grey bars represent the negative control with no InlA and incubation with 20 μg/mL peptide. Binding peptides were detected by ELISA using anti-FITC:HRP conjugate and measurement of absorbance at 450 nm. Means of three independent measurements and their standard deviations are shown. JB1: GLHTSATNLYLH (A), JB2: DSQFNKYSIATV (B), JB3: SGVYKVAYDWQH (C), JB4: QFDYMRPANDTH (D), JB5: SNSIDKVNRPIN (E).
From: Kenzel, J.; Brüggemann, D.A.; Funke, S.A.
Selection of Listeria monocytogenes InlA-Binding Peptides Using Phage Display—Novel Compounds for Diagnostic Applications? Appl. Microbiol. 2022, 2, 921-933.
doi: 10.3390/applmicrobiol2040070
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Sheep anti FITC antibody (640005) used to detect bound FAM labeled peptides by ELISA
Image caption:
InlA-binding peptides JB1 and JB2 interact with L. monocytogenes. Bacteria, either L. monocytogenes serotype 1/2a, L. monocytogenes serotype 4b (A) or E. coli (B) was incubated with FAM-labeled peptides JB1 (GLHTSATNLYLH), JB2 (DSQFNKYSIATV), JB3 (SGVYKVAYDWQH), JB4 (QFDYMRPANDTH) und JB5 (SNSIDKVNRPIN) in a final concentration of 0 μg/mL, 20 μg/mL and 50 μg/mL. Binding peptides were detected by ELISA using anti-FITC:HRP conjugate and measurement of absorbance at 450 nm. Averages and standard deviations of three independent measurements are shown.
From: Kenzel, J.; Brüggemann, D.A.; Funke, S.A.
Selection of Listeria monocytogenes InlA-Binding Peptides Using Phage Display—Novel Compounds for Diagnostic Applications? Appl. Microbiol. 2022, 2, 921-933.
doi: 10.3390/applmicrobiol2040070
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- FITC
Antibody Quality and Performance Guarantee
| Sheep anti FITC antibody recognizes the fluorochrome Fluorescein Isothiocyanate (FITC). This reagent may be useful for amplification of staining using FITC conjugated reagents. |
- Target Species
- Chemical
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography.
- Antiserum Preparation
- Antiserum to FITC was raised by repeated immunisation of sheep with highly purified antigen.
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Fluorescein isothiocyanate (isomer 1).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Sheep IgG (H/L):Biotin | ||||||
References for FITC antibody
-
Heine, S. et al. (2011) CNGA3: A Target of Spinal Nitric Oxide/cGMP Signaling and Modulator of Inflammatory Pain Hypersensitivity.
J Neurosci. 31: 11184-92. -
Martínez-Sernández V et al. (2016) Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS One. 11 (6): e0156530. -
Aillaud, I. et al. (2022) A novel D-amino acid peptide with therapeutic potential (ISAD1) inhibits aggregation of neurotoxic disease-relevant mutant Tau and prevents Tau toxicity in vitro..
Alzheimers Res Ther. 14 (1): 15. -
Kenzel, J. et al. (2022) Selection of Listeria monocytogenes InlA-Binding Peptides Using Phage Display—Novel Compounds for Diagnostic Applications?
Applied Microbiology. 2 (4): 921-933. -
Abouzayed, A. et al. (2023) Co-injection of anti-HER2 antibody Trastuzumab does not increase efficacy of [(177)Lu]Lu-PSMA-617 therapy in an animal model of prostate cancer.
Am J Nucl Med Mol Imaging. 13 (3): 107-17.
- Synonyms
- Fluorescein Isothiocyanate
- RRID
- AB_620733
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