FITC antibody
Rabbit anti FITC antibody (4510-7804) used as a visualization antibody in an ELISA assay for the detection of anti Fasciola hepatica antibodies in human sera.
Image caption:
Comparison of the detection limit of the MM3-SERO and SeroFluke tests. The ability of MM3-SERO ELISA (A) and SeroFluke (B) to detect anti-Fasciola antibodies in two fold dilutions of pooled positive sera was compared. The starting serum dilution was 1/100 for both tests.
From: Martínez-Sernández V, Muiño L, Perteguer MJ, Gárate T, Mezo M, González-Warleta M, et al. (2011)
Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis.
PLoS Negl Trop Dis 5(11): e1376.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- FITC
Antibody Quality and Performance Guarantee
| Rabbit anti FITC antibody recognizes fluorescein and does not react with immunoglobulins from any species. Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. |
- Target Species
- Chemical
- Product Form
- Purified Ig - liquid
- Preparation
- Purified IgG prepared by immunoaffinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- FITC Isomer 1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
References for FITC antibody
-
van der Loos, C. (2008) Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.
J. Histochem Cytochem. 56: 313-328. -
Bdeir, K. et al. (2010) Neutrophil alpha-defensins cause lung injury by disrupting the capillary-epithelial barrier.
Am J Respir Crit Care Med. 181: 935-46. -
Hsiao, J.R. et al. (2010) Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.
Head Neck. 32 (2): 160-72. -
Türeci, ö et al. (2011) Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals.
Gene. 481 (2): 83-92. -
Martínez-Sernández, V. et al. (2011) Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.
PLoS Negl Trop Dis. 5 (11): e1376. -
Martínez-Sernández, V. et al. (2014) The MF6p/FhHDM-1 major antigen secreted by the trematode parasite Fasciola hepatica is a heme-binding protein.
J Biol Chem. 289 (3): 1441-56. -
Dileepan, T. et al. (2015) In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues.
PLoS One. 10 (6): e0128862. -
Arni, S. et al. (2021) Subnormothermic ex vivo. lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors.
PLoS One. 16 (8): e0255155.
View The Latest Product References
- Synonyms
- Fluorescein Isothiocyanate
- RRID
- AB_619423
View more products with FITC specificity
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