FITC antibody

Rabbit anti FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FITC

Product Code Applications Pack Size List Price Your Price Qty
4510-7804
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SDS Safety Datasheet SDS
C E 1 mg loader
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Rabbit anti FITC antibody recognizes fluorescein and does not react with immunoglobulins from any species.

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins.

Target Species
Chemical
Product Form
Purified Ig - liquid
Preparation
Purified IgG prepared by immunoaffinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
FITC Isomer 1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for FITC antibody

  1. van der Loos, C. (2008) Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.
    J. Histochem Cytochem. 56: 313-328.
  2. Bdeir, K. et al. (2010) Neutrophil alpha-defensins cause lung injury by disrupting the capillary-epithelial barrier.
    Am J Respir Crit Care Med. 181: 935-46.
  3. Hsiao, J.R. et al. (2010) Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.
    Head Neck. 32 (2): 160-72.
  4. Türeci O et al. (2011) Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals.
    Gene. 481 (2): 83-92.
  5. Martínez-Sernández, V. et al. (2011) Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.
    PLoS Negl Trop Dis. 5 (11): e1376.
  6. Martínez-Sernández, V. et al. (2014) The MF6p/FhHDM-1 major antigen secreted by the trematode parasite Fasciola hepatica is a heme-binding protein.
    J Biol Chem. 289 (3): 1441-56.
  7. Dileepan, T. et al. (2015) In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues.
    PLoS One. 10 (6): e0128862.
  8. Arni, S. et al. (2021) Subnormothermic ex vivo. lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors.
    PLoS One. 16 (8): e0255155.
  9. View The Latest Product References

ELISA

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
Fluorescein Isothiocyanate
RRID
AB_619423
View more products with FITC specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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