FITC antibody

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Rabbit anti FITC:HRP

Rabbit anti FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4510-7864 C E WB 1 ml
4510-7804 C E 1 mg
Rabbit anti FITC antibody recognizes fluorescein and does not react with immunoglobulins from any species.

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins.

Product Details

Target Species
Chemical
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified Ig - liquid
Preparation
Purified IgG prepared by affinity chromatography.
Preparation
Purified IgG prepared by immunoaffinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Thiomersal
1% Bovine serum albumin.
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
FITC Isomer 1.
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of FITC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/200 1/1000
Immunohistology - Frozen 1/20 1/200
Western Blotting 1/20 1/200
ELISA
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for FITC antibody

  1. van der Loos, C. (2008) Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.
    J. Histochem Cytochem. 56: 313-328.
  2. Bdeir, K. et al. (2010) Neutrophil alpha-defensins cause lung injury by disrupting the capillary-epithelial barrier.
    Am J Respir Crit Care Med. 181: 935-46.
  3. Hsiao, J.R. et al. (2010) Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.
    Head Neck. 32 (2): 160-72.
  4. Türeci O et al. (2011) Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals.
    Gene. 481 (2): 83-92.
  5. Martínez-Sernández, V. et al. (2011) Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.
    PLoS Negl Trop Dis. 5 (11): e1376.
  6. Martínez-Sernández, V. et al. (2014) The MF6p/FhHDM-1 major antigen secreted by the trematode parasite Fasciola hepatica is a heme-binding protein.
    J Biol Chem. 289 (3): 1441-56.
  7. Dileepan, T. et al. (2015) In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues.
    PLoS One. 10 (6): e0128862.