IgG1 antibody
Horseradish peroxidase conjugated Goat anti Canine IgG1 (AHP947P) antibody used to evaluate the immunoglobulin response to Leishmania infantum promastigotes by sympomatic and asymptomatic dogs by western blotting.
Image caption:
IgG reaction pattern associated with CVL status.
Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side.
From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016)
Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS ONE 11(2): e0149894.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetGoat anti Dog IgG1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG1
Antibody Quality and 
Unrivaled 
| Goat anti Dog IgG1 polyclonal antibody specifically recognises canine IgG1 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins. Goat anti Dog IgG1 may cross react with IgG1 from other species. |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to canine IgG1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/10,000 |
| Immunohistology - Frozen | |
1/200 | 1/2000 |
| Western Blotting | |
1/1000 | 1/10,000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
References for IgG1 antibody
-
Bird, R.C. et al. (2011) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
Cancer Immunol Immunother. 60 (1): 87-97. -
Agallou, M. et al. (2016) Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS One. 11 (2): e0149894. -
Martínez Abad, L.P. et al. (2017) Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.
Acta Trop. 166: 133-8. -
Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
Vet Dermatol. 31 (2): 111-115.
- RRID
- AB_2249180
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