Digoxigenin antibody
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Sheep anti digoxigenin antibody recognizes digoxigenin, a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production.
This antibody cross reacts with digoxin and digitoxin. Negligible reactivity has been observed with ouabain.
This antibody cross reacts with digoxin and digitoxin. Negligible reactivity has been observed with ouabain.
Product Details
- Target Species
- Chemical
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by Protein G affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Digoxin-3-CM conjugated to keyhole limpet haemocyanin.
- Approx. Protein Concentrations
- 5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Digoxigenin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/5,000 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Digoxigenin antibody
-
Mori, K. et al. (2007) The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma.
Int. Immunol. 19: 745-53. -
Cross, S.J. et al. (2016) Transverse Magnetic Tweezers Allowing Coincident Epifluorescence Microscopy on Horizontally Extended DNA.
Methods Mol Biol. 1431: 73-90. -
Hu, W.W. et al. (2009) Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces.
J Control Release. 135 (3): 250-8.
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