Digoxigenin antibody

Sheep anti Digoxigenin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Digoxigenin

Product Code Applications Pack Size List Price Your Price Qty
3210-0488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF WB 1 ml
List Price Your Price
Search for Batch Specific Datasheets

Sheep anti digoxigenin antibody recognizes digoxigenin, a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production.

This antibody cross reacts with digoxin and digitoxin. Negligible reactivity has been observed with ouabain.

Target Species
Chemical
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by Protein G affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Digoxin-3-CM conjugated to keyhole limpet haemocyanin.
Approx. Protein Concentrations
5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/5,000
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
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Description Rabbit anti Sheep IgG (H/L):Biotin
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
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Description Donkey anti Sheep/Goat IgG:DyLight®488
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader
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Description Donkey anti Sheep/Goat IgG:HRP

References for Digoxigenin antibody

  1. Mori, K. et al. (2007) The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma.
    Int. Immunol. 19: 745-53.
  2. Cross, S.J. et al. (2016) Transverse Magnetic Tweezers Allowing Coincident Epifluorescence Microscopy on Horizontally Extended DNA.
    Methods Mol Biol. 1431: 73-90.
  3. Hu, W.W. et al. (2009) Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces.
    J Control Release. 135 (3): 250-8.
  4. Klebes, A. et al. (2021) Multianalyte lateral flow immunoassay for simultaneous detection of protein-based inflammation biomarkers and pathogen DNA
    Sensors and Actuators B: Chemical. : 131283.
  5. Toleikis, A. et al. (2018) oriD structure controls RepD initiation during rolling-circle replication
    Sci Rep. 8: 1206.
  6. Brunauer, A. et al. (2021) Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.
    Biosensors (Basel). 11(3):74.

RRID
AB_620732
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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