Digoxigenin antibody
Sheep anti digoxigenin antibody (3210-0488) used to anchor digoxigenin tagged DNA to a slide surface in a "magentic tweezer" biosensor.
Image caption:
Magnetic tweezers RepD nicking assay. A 1 μμm diameter, paramagnetic bead is attached by multiple biotin-streptavidin linkages to one end of a dsDNA (4 kb or 10 kb) molecule that has the initiation site, oriD, within its central region. Multiple digoxigenin-anti-digoxigenin linkages attach the other end to a glass microscope coverslip. Application of a magnetic field, generated by a pair of permanent magnets, causes the bead to rise from the microscope coverslip surface, extending the DNA molecule. Rotation of the magnetic field causes bead rotation and DNA supercoiling. At low force, the dsDNA first becomes twisted and then undergoes plectoneme formation (DNA writhe) resulting in reduction in DNA end-to-end length and bead motion towards the coverslip surface. When RepD nicks the DNA, supercoiling is relaxed and the bead moves rapidly upwards, towards its extended length.
From:Toleikis, A., Webb, M.R. & Molloy, J.E.
oriD structure controls RepD initiation during rolling-circle replication.
Sci Rep 8, 1206 (2018).
doi: 10.1038/s41598-017-18817-6
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti digoxigenin antibody used to bind digoxigenin pathogen amplicons in a lateral flow assay for P. aeruginosa. in wound exudates.
Image caption:
Principle of the paper-based approach for P. aeruginosa detection in wound exudate. (A) Wound exudate spiked with P. aeruginosa is transferred into a top-screw tube containing glass beads and the pathogen is lysed via bead beating. (B) Then, the crude lysate is transferred to the RPA reaction. Target DNA and IAC-DNA are amplified and double-labeled target DNA amplicons and double-labeled IAC-DNA amplicons are generated. (C) Anti-digoxigenin-conjugate fluorescent beads are binding to the amplicons and they are detected via lateral flow assay. (D) Schematic drawing of the NALFIA. The double-labeled target DNA amplicon binds to the test line (TL) leading to a positive test result. The double-labeled IAC-DNA amplicon binds to a separate control line (IAC) to exclude false negative results, and a flow control (FC) shows whether the sample was processed.
From: Brunauer A, Verboket RD, Kainz DM, von Stetten F, Früh SM.
Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.
Biosensors (Basel). 2021 Mar 6;11(3):74.
doi: 10.3390/bios11030074.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti digoxigenin antibody (3210-0488) used to bind digoxigenin pathogen amplicons in a lateral flow assay for P. aeruginosa. in wound exudates.
Image caption:
Lysis control and specificity of the paper-based approach for P. aeruginosa detection. P. aeruginosa was spiked into (A) PBS or (B) wound exudate, whereas the negative control (i) contains no bacteria. The spiked samples were added without (ii) or after bead beating (2 × 20 s (iii) to the RPA reaction and the amplification products were detected via NALFIA. The NALFIA showed a clear signal at the test line (TL) for the lysed sample. Only a weak signal was observed for the sample that was not lysed.
From: Brunauer A, Verboket RD, Kainz DM, von Stetten F, Früh SM.
Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.
Biosensors (Basel). 2021 Mar 6;11(3):74.
doi: 10.3390/bios11030074.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti digoxigenin antibody (3210-0488) used to bind digoxigenin pathogen amplicons in a lateral flow assay for P. aeruginosa. in wound exudates.
Image caption:
Specificity of the paper-based approach for the detection of P. aeruginosa over other pathogens present in wounds. The following pathogens were spiked into PBS: Without bacteria (i), P. aeruginosa (ii), S. aureus (iii), S. epidermidis (iv), S. agalactiae (v), E. coli (vi), K. pneumoniae (vii), E. faecalis (viii), P. mirabilis (ix). The samples were lysed via bead beating, and the lysate was added directly to the RPA reaction. Subsequently, the amplification products were detected via NALFIA. The test line (TL) showed a positive signal for the sample spiked with P. aeruginosa, whereas the signals for the other pathogens were below the fluorescence intensity of the detection limit. Signals for internal amplification control (IAC) and flow control (FC) were observed for all NALFIA strips. The experiments were conducted three times and were performed in triplicates.
From: Brunauer A, Verboket RD, Kainz DM, von Stetten F, Früh SM.
Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.
Biosensors (Basel). 2021 Mar 6;11(3):74.
doi: 10.3390/bios11030074.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
BS LF ResetSheep anti Digoxigenin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Digoxigenin
Antibody Quality and Performance Guarantee
| Sheep anti digoxigenin antibody recognizes digoxigenin, a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. This antibody cross reacts with digoxin and digitoxin. Negligible reactivity has been observed with ouabain. |
- Target Species
- Chemical
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by Protein G affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Digoxin-3-CM conjugated to keyhole limpet haemocyanin.
- Approx. Protein Concentrations
- 5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/5,000 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Rabbit anti Sheep IgG (H/L):Biotin | ||||||
References for Digoxigenin antibody
-
Mori, K. et al. (2007) The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma.
Int. Immunol. 19: 745-53. -
Hu, W.W. et al. (2009) Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces.
J Control Release. 135 (3): 250-8. -
Cross, S.J. et al. (2016) Transverse Magnetic Tweezers Allowing Coincident Epifluorescence Microscopy on Horizontally Extended DNA.
Methods Mol Biol. 1431: 73-90. -
Toleikis, A. et al. (2018) oriD structure controls RepD initiation during rolling-circle replication
Sci Rep. 8: 1206. -
Klebes, A. et al. (2021) Multianalyte lateral flow immunoassay for simultaneous detection of protein-based inflammation biomarkers and pathogen DNA
Sensors and Actuators B: Chemical. : 131283. -
Brunauer, A. et al. (2021) Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.
Biosensors (Basel). 11(3):74.
- RRID
- AB_620732
3210-0488
153692
156182
156789
166338
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