Leukemia Inhibitory Factor antibody

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Chicken anti Bovine Leukemia Factor:Biotin

Chicken anti Bovine Leukemia Factor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2381B E WB 50 µg
AHP2381 E WB 0.1 mg
Chicken anti Bovine leukemia inhibitory factor antibody recognizes bovine leukemia inhibitory factor (LIF), a 22 kDa cytokine belonging to the IL-6 cytokine family.

Bovine LIF is a cytokine that regulates differentiation in a range of cell types including myeloid and neuronal cells, and in particular induction of terminal differentiation in myeloid leukemia cells.

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine leukemia inhibitory factor (LIF) were raised by repeated immunisation of chickens with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant bovine leukemia inhibitory factor
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
Q27956 Related reagents
Entrez Gene
LIF Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of Leukemia Inhibitory Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.05ug/ml 0.5ug/ml
Western Blotting
ELISA 1ug/ml 5ug/ml
Western Blotting 0.1ug/ml 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection antibody in a sandwich ELISA together with AHP2381 as the capture reagent. Recombinant Bovine leukemia inhibitory factor (PBP036) may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2381B as the detection reagent. Recombinant Bovine leukemia inhibitory factor (PBP036) may be used as a standard.