Leukemia Inhibitory Factor antibody
Recombinant Bovine leukemia inhibitory factor (PBP036) detected using Chicken anti Bovine leukemia inhibitory factor (AHP2381) as the capture reagent and Chicken anti Bovine leukemia inhibitory factor:Biotin (AHP2381B) as the detection reagent followed by Streptavidin:HRP.
Chicken anti Bovine leukemia inhibitory factor antibody recognizes bovine leukemia inhibitory factor (LIF), a 22 kDa cytokine belonging to the IL-6 cytokine family.
Bovine LIF is a cytokine that regulates differentiation in a range of cell types including myeloid and neuronal cells, and in particular induction of terminal differentiation in myeloid leukemia cells (Gough et al. 1989).
Bovine LIF is a cytokine that regulates differentiation in a range of cell types including myeloid and neuronal cells, and in particular induction of terminal differentiation in myeloid leukemia cells (Gough et al. 1989).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine leukemia inhibitory factor (LIF) were raised by repeated immunisation of chickens with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant bovine leukemia inhibitory factor
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q27956 Related reagents
- Entrez Gene
- LIF Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of Leukemia Inhibitory Factor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.05ug/ml | 0.5ug/ml | |
| Western Blotting | |||
| ELISA | 1ug/ml | 5ug/ml | |
| Western Blotting | 0.1ug/ml | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection antibody in a sandwich ELISA together with AHP2381 as the capture reagent. Recombinant Bovine leukemia inhibitory factor (PBP036) may be used as a standard.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2381B as the detection reagent. Recombinant Bovine leukemia inhibitory factor (PBP036) may be used as a standard.
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