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IgG antibody

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Anti Bovine IgG Antibody thumbnail image 1 Anti Bovine IgG Antibody thumbnail image 2 Anti Bovine IgG Antibody thumbnail image 3 Anti Bovine IgG Antibody thumbnail image 4 Anti Bovine IgG Antibody thumbnail image 5 Anti Bovine IgG Antibody thumbnail image 6
Anti Bovine IgG Antibody gallery image 1
Published customer image:
Sheep anti Bovine IgG polyclonal antibody (AAI23) used for the the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP-specific Ig titres following primary and boost immunisations with TNP-conjugated antigens. Five calves were subcutaneously immunised with 3 mg TNP-CGG or TNP-AECM-FICOLL in QuilA then boosted 29 days later with the same immunising antigens. The dotted vertical line indicates when the booster TNP-CGG/TNP-AECM-FICOLL immunisations were given (day 29 pi). Blood samples were taken at various time-points following these injections and TNP-specific IgG (diamond symbols) and IgM (square symbols) titres were determined by ELISA in calves from the T-D group (open symbols) or T-I group (filled symbols). Results are expressed as the mean of determinations from individual calves in each group±SEM, n = 5.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B.
Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
Vet Res. 2012 Oct 10;43:68.
Anti Bovine IgG Antibody gallery image 2
Published customer image:
Sheep anti Bovine IgG polyclonal antibody (AAI23) used for the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
TNP-specific plasma and memory B cell detection post-boost immunization with TNP-CGG (T-D group). Five calves were subcutaneously immunized with 3 mg TNP-CGG in QuilA then boosted 29 days later with the same immunizing antigen. The dotted vertical line indicates when the booster TNP-CGG immunization was given (day 29 pi). Blood samples were taken at various time-points following the immunisations. TNP-specific IgG, plasma cell (filled circles, left-axis) and memory B cell (open circles, right-axis) numbers were determined by B cell ELISPOT. Results were expressed as ASC per 106 PBMC for plasma cells and induced ASC per 106 cultured PBMC for memory B cells (mean of determinations from individual calves in each group, n = 5).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B.
Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
Vet Res. 2012 Oct 10;43:68.
Anti Bovine IgG Antibody gallery image 3
Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
Kinetics of antigen-specific IgG secreting plasma cells and memory B cells post-TTC immunization. At day 134 post-primary TNP-conjugated immunization, all calves were subcutaneously immunized with 3 mg TTC in IFA then boosted 63 days later with the same immunizing antigen. Blood samples were taken at various time-points following these immunisations. The time of primary and boost TTC immunisations are indicated by vertical dotted lines (day 134 and 197 pi, respectively). Antigen-specific IgG, plasma cell (A) and memory B cell (B) numbers were determined by B cell ELISPOT and are identified as follows: CGG-specific (open triangle), TTC-specific (crosses) and TNP-specific (closed triangle) IgG secreting ASCs. Results were expressed as ASC per 106 PBMC for plasma cells and induced ASC per 106 cultured PBMC for memory B cells (mean of determinations from individual calves in each group for the TNP- and CGG-specific responses, n = 5, and mean of determinations from all calves for the TTC-specific responses, n = 10). a = p < 0.05, b = p < 0.001.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.
Anti Bovine IgG Antibody gallery image 4
Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP- and TTC-specific IgG titres following primary and boost TTC immunisations. TNP-specific IgG titres in calves from the T-D group (open diamonds) or T-I group (filled diamonds) and TTC-specific IgG titres in all calves (cross symbols) were determined by ELISA. The dotted vertical lines indicate when the primary and boost TTC immunisations were administered (days 134 and 197 pi, respectively). Results are expressed as the mean of determinations from individual calves in each group for the TNP-specific responses (n = 5) and the mean of determinations from all calves for the TTC-specific responses (n = 10).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.
Anti Bovine IgG Antibody gallery image 5
Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the measurement of cattle IgG responses to tsetse fly antigens by ELISA.
Image caption:
Cattle IgG responses against WSE and, Tsal152–75 and Tsal1145–166 peptides. The IgG responses directed against whole saliva extracts (WSE) and the two candidate synthetic peptides were investigated in 43 animals from a tsetse infested area, 17 animals from a tsetse free area and four animals exposed experimentally to A. variegatum, An. gambiae, Tabanidae spp. or Stomoxys spp. (other arthropods). Individual =ΔOD values are represented by empty circles. In the scatter plot, the horizontal bars indicate the median value for each group. Statistical significance between the different groups is indicated (non-parametric Mann–Whitney U-test)

From: Somda MB, Cornelie S, Bengaly Z, Mathieu-Daudé F, Poinsignon A, Dama E, Bouyer J, Sidibé I, Demettre E, Seveno M, Remoué F, Sanon A, Bucheton B.
Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
Parasit Vectors. 2016 Mar 15;9(1):149.
Anti Bovine IgG Antibody gallery image 6
Published customer image:
Biotin conjugated Sheep anti Bovine IgG antibody (AAI23B) used for the detection of M. avium subsp. paratuberculosis protein specific IgG secreted by cells from infected calves by ELISPOT along with biotinylated Sheep anti Bovine IgA antibody used to evaluate similar IgA responses.
Image caption:
Net IgA-ASC and IgG-ASC responses to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in mid-jejunal segments and assayed for IgA (A) and IgG (B) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in terminal small intestinal segments and assayed for IgG (C) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. The net number of ASC specific to each M. avium subsp. paratuberculosis protein in infected compartments was calculated using the formula provided in Materials and Methods. M. avium subsp. paratuberculosis proteins for which all calves (n=3) had detectable net ASC responses are shown with significant (P <0.05) increases in the frequency of M. avium subsp. paratuberculosis-protein specific ASCs denoted by an asterisk. Data presented are mean values with SEM.

From: Facciuolo A, Gonzalez-Cano P, Napper S, Griebel PJ, Mutharia LM (2016)
Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer’s Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
>PLoS ONE 11(7): e0158747.

Sheep anti Bovine IgG:Alk. Phos.

Sheep anti Bovine IgG:Biotin

Sheep anti Bovine IgG:FITC

Sheep anti Bovine IgG:HRP

Sheep anti Bovine IgG

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAI23AB
Datasheet
E WB
SDS
0.5 mg loader

AAI23B
Datasheet
E WB
SDS
1 mg loader

AAI23F
Datasheet
C F
SDS
1 mg loader

AAI23P
Datasheet
E WB
SDS
1 mg loader

AAI23
Datasheet
E ID
SDS
1 mg loader

Sheep anti Bovine IgG polyclonal antibody recognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other species

Sheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrieling et al. 2013).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG fraction conjugated to Biotin - liquid
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.05% Proclin™ 300
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgG.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/50000
Western Blotting 1/5000 1/50000
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
ELISA 1/10,000 1/100,000
Western Blotting
ELISA 1/100 1/30,000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgG antibody

  1. Santema, W.J. (1982) Hsp70 as a candidate subunit vaccine for paratuberculosis
    Dissertation, University of Utrecht.
  2. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.
    Clin Vaccine Immunol. 20: 1669-74.
  3. Vrieling, M. et al. (2013) Hsp70 vaccination-induced primary immune responses in efferent lymph of the draining lymph node.
    Vaccine. 31 (42): 4720-7.
  4. Bridger, P.S. et al. (2011) Detection of colostrum-derived alloantibodies in calves with bovine neonatal pancytopenia.
    Vet Immunol Immunopathol. 141: 1-10.
  5. Grant, C.F. et al. (2012) Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
    Vet Res. 43: 68.
  6. Naylor, S.W. et al. (2007) Impact of the direct application of therapeutic agents to the terminal recta of experimentally colonized calves on Escherichia coli O157:H7 shedding.
    Appl Environ Microbiol. 73: 1493-500.
  7. Somda, M.B. et al. (2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.
    Vet Parasitol. 197 (1-2): 332-40.
  8. Hosking, C.G. et al. (2015) Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum.
    Int J Parasitol. 45 (11): 729-40.
  9. Somda, M.B. et al. (2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
    Parasit Vectors. 9 (1): 149.
  10. Facciuolo, A. et al. (2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
    PLoS One. 11 (7): e0158747.
  11. Subharat, S. et al. (2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.
    Vet Immunol Immunopathol. 164 (3-4): 201-7.
  12. Benedictus, L. et al. (2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.
    Vaccine. 34 (8): 1002-5.
  13. Denholm, S.J. et al. (2018) Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements.
    J Dairy Sci. 101 (11): 10248-10258.
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