IgG antibody

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Sheep anti Bovine IgG:Alk. Phos.

Sheep anti Bovine IgG:Biotin

Sheep anti Bovine IgG:FITC

Sheep anti Bovine IgG:HRP

Sheep anti Bovine IgG

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI23AB E WB 0.5 mg
AAI23B E WB 1 mg
AAI23F C F 1 mg
AAI23P E WB 1 mg
AAI23 E ID 1 mg
Sheep anti Bovine IgG polyclonal antibody recognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other species

Sheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrieling et al. 2013).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG fraction conjugated to Biotin - liquid
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.1% Proclin™ 300
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgG.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/50000
Western Blotting 1/5000 1/50000
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
ELISA 1/10,000 1/100,000
Western Blotting
ELISA 1/100 1/10,000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Santema, W.J. (1982) Hsp70 as a candidate subunit vaccine for paratuberculosis
    Dissertation, University of Utrecht.
  2. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.
    Clin Vaccine Immunol. 20: 1669-74.
  3. Vrieling, M. et al. (2013) Hsp70 vaccination-induced primary immune responses in efferent lymph of the draining lymph node.
    Vaccine. 31 (42): 4720-7.
  4. Bridger, P.S. et al. (2011) Detection of colostrum-derived alloantibodies in calves with bovine neonatal pancytopenia.
    Vet Immunol Immunopathol. 141: 1-10.
  5. Grant, C.F. et al. (2012) Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
    Vet Res. 43: 68.
  6. Naylor, S.W. et al. (2007) Impact of the direct application of therapeutic agents to the terminal recta of experimentally colonized calves on Escherichia coli O157:H7 shedding.
    Appl Environ Microbiol. 73: 1493-500.
  7. Somda, M.B. et al. (2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.
    Vet Parasitol. 197 (1-2): 332-40.
  8. Hosking, C.G. et al. (2015) Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum.
    Int J Parasitol. 45 (11): 729-40.
  9. Somda, M.B. et al. (2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
    Parasit Vectors. 9 (1): 149.
  10. Facciuolo, A. et al. (2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
    PLoS One. 11 (7): e0158747.
  11. Subharat, S. et al. (2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.
    Vet Immunol Immunopathol. 164 (3-4): 201-7.
  12. Benedictus, L. et al. (2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.
    Vaccine. 34 (8): 1002-5.