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IgG antibody

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Anti Bovine IgG Antibody thumbnail image 1 Anti Bovine IgG Antibody thumbnail image 2 Anti Bovine IgG Antibody thumbnail image 3 Anti Bovine IgG Antibody thumbnail image 4 Anti Bovine IgG Antibody thumbnail image 5 Anti Bovine IgG Antibody thumbnail image 6 Anti Bovine IgG Antibody thumbnail image 7 Anti Bovine IgG Antibody thumbnail image 8
Anti Bovine IgG Antibody gallery image 1
Published customer image:
Sheep anti Bovine IgG polyclonal antibody (AAI23) used for the the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP-specific Ig titres following primary and boost immunisations with TNP-conjugated antigens. Five calves were subcutaneously immunised with 3 mg TNP-CGG or TNP-AECM-FICOLL in QuilA then boosted 29 days later with the same immunising antigens. The dotted vertical line indicates when the booster TNP-CGG/TNP-AECM-FICOLL immunisations were given (day 29 pi). Blood samples were taken at various time-points following these injections and TNP-specific IgG (diamond symbols) and IgM (square symbols) titres were determined by ELISA in calves from the T-D group (open symbols) or T-I group (filled symbols). Results are expressed as the mean of determinations from individual calves in each group±SEM, n = 5.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B.
Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
Vet Res. 2012 Oct 10;43:68.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 2
Published customer image:
Sheep anti Bovine IgG polyclonal antibody (AAI23) used for the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
TNP-specific plasma and memory B cell detection post-boost immunization with TNP-CGG (T-D group). Five calves were subcutaneously immunized with 3 mg TNP-CGG in QuilA then boosted 29 days later with the same immunizing antigen. The dotted vertical line indicates when the booster TNP-CGG immunization was given (day 29 pi). Blood samples were taken at various time-points following the immunisations. TNP-specific IgG, plasma cell (filled circles, left-axis) and memory B cell (open circles, right-axis) numbers were determined by B cell ELISPOT. Results were expressed as ASC per 106 PBMC for plasma cells and induced ASC per 106 cultured PBMC for memory B cells (mean of determinations from individual calves in each group, n = 5).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B.
Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
Vet Res. 2012 Oct 10;43:68.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 3
Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
Kinetics of antigen-specific IgG secreting plasma cells and memory B cells post-TTC immunization. At day 134 post-primary TNP-conjugated immunization, all calves were subcutaneously immunized with 3 mg TTC in IFA then boosted 63 days later with the same immunizing antigen. Blood samples were taken at various time-points following these immunisations. The time of primary and boost TTC immunisations are indicated by vertical dotted lines (day 134 and 197 pi, respectively). Antigen-specific IgG, plasma cell (A) and memory B cell (B) numbers were determined by B cell ELISPOT and are identified as follows: CGG-specific (open triangle), TTC-specific (crosses) and TNP-specific (closed triangle) IgG secreting ASCs. Results were expressed as ASC per 106 PBMC for plasma cells and induced ASC per 106 cultured PBMC for memory B cells (mean of determinations from individual calves in each group for the TNP- and CGG-specific responses, n = 5, and mean of determinations from all calves for the TTC-specific responses, n = 10). a = p < 0.05, b = p < 0.001.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 4
Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP- and TTC-specific IgG titres following primary and boost TTC immunisations. TNP-specific IgG titres in calves from the T-D group (open diamonds) or T-I group (filled diamonds) and TTC-specific IgG titres in all calves (cross symbols) were determined by ELISA. The dotted vertical lines indicate when the primary and boost TTC immunisations were administered (days 134 and 197 pi, respectively). Results are expressed as the mean of determinations from individual calves in each group for the TNP-specific responses (n = 5) and the mean of determinations from all calves for the TTC-specific responses (n = 10).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 5
Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the measurement of cattle IgG responses to tsetse fly antigens by ELISA.
Image caption:
Cattle IgG responses against WSE and, Tsal152–75 and Tsal1145–166 peptides. The IgG responses directed against whole saliva extracts (WSE) and the two candidate synthetic peptides were investigated in 43 animals from a tsetse infested area, 17 animals from a tsetse free area and four animals exposed experimentally to A. variegatum, An. gambiae, Tabanidae spp. or Stomoxys spp. (other arthropods). Individual =ΔOD values are represented by empty circles. In the scatter plot, the horizontal bars indicate the median value for each group. Statistical significance between the different groups is indicated (non-parametric Mann–Whitney U-test)

From: Somda MB, Cornelie S, Bengaly Z, Mathieu-Daudé F, Poinsignon A, Dama E, Bouyer J, Sidibé I, Demettre E, Seveno M, Remoué F, Sanon A, Bucheton B.
Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
Parasit Vectors. 2016 Mar 15;9(1):149.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 6
Published customer image:
Biotin conjugated Sheep anti Bovine IgG antibody (AAI23B) used for the detection of M. avium subsp. paratuberculosis protein specific IgG secreted by cells from infected calves by ELISPOT along with biotinylated Sheep anti Bovine IgA antibody used to evaluate similar IgA responses.
Image caption:
Net IgA-ASC and IgG-ASC responses to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in mid-jejunal segments and assayed for IgA (A) and IgG (B) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in terminal small intestinal segments and assayed for IgG (C) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. The net number of ASC specific to each M. avium subsp. paratuberculosis protein in infected compartments was calculated using the formula provided in Materials and Methods. M. avium subsp. paratuberculosis proteins for which all calves (n=3) had detectable net ASC responses are shown with significant (P <0.05) increases in the frequency of M. avium subsp. paratuberculosis-protein specific ASCs denoted by an asterisk. Data presented are mean values with SEM.

From: Facciuolo A, Gonzalez-Cano P, Napper S, Griebel PJ, Mutharia LM (2016)
Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer’s Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
>PLoS ONE 11(7): e0158747.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 7
Published customer image:
Horseradish peroxidase conjugated Sheep anti Bovine IgG antibody (AAI23P) used as a detection antibody in an ELISA to evaluate IgG responses to D.viviparus. infection.
Image caption:
Anti-rMSP antibody development during vaccination trials. Mean optical density (OD) values are displayed over the course of the study for immunoglobulin (Ig) classes IgG, IgA and IgM for the different study groups. Error bars indicate standard deviations. V = vaccination, C = challenge infection.

From: Springer, A.; von Holtum, C.; von Samson-Himmelstjerna, G.; Strube, C.
Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus.
Pathogens 2022, 11, 55.
doi: 10.3390/pathogens11010055
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine IgG Antibody gallery image 8
Published customer image:
Horseradish peroxidase conjugated Sheep anti Bovine IgG antibody (AAI23P) used as a detection antibody in an ELISA to evaluate IgG responses to D.viviparus. infection.
Image caption:
Anti-rMSP antibody development during vaccination trials. Mean optical density (OD) values are displayed over the course of the study for immunoglobulin (Ig) classes IgG, IgA and IgM for the different study groups. Error bars indicate standard deviations. V = vaccination, C = challenge infection.

From: Springer, A.; von Holtum, C.; von Samson-Himmelstjerna, G.; Strube, C.
Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus.
Pathogens 2022, 11, 55.
doi: 10.3390/pathogens11010055
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Sheep anti Bovine IgG

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Product Code Applications Pack Size List Price Your Price Qty
AAI23
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ID 1 mg loader
List Price Your Price
loader

Sheep anti Bovine IgG polyclonal antibody recognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other species

Sheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrieling et al. 2013).

Target Species
Bovine
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/30,000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
loader
Description Rabbit anti Sheep IgG (H/L):Biotin

References for IgG antibody

  1. Naylor, S.W. et al. (2007) Impact of the direct application of therapeutic agents to the terminal recta of experimentally colonized calves on Escherichia coli O157:H7 shedding.
    Appl Environ Microbiol. 73: 1493-500.
  2. Cortes, H.C. et al. (2007) Application of conventional and real-time fluorescent ITS1 rDNA PCR for detection of Besnoitia besnoiti. infections in bovine skin biopsies.
    Vet Parasitol. 146 (3-4): 352-6.
  3. Bridger, P.S. et al. (2011) Detection of colostrum-derived alloantibodies in calves with bovine neonatal pancytopenia.
    Vet Immunol Immunopathol. 141: 1-10.
  4. Waap, H. et al. (2011) A modified agglutination test for the diagnosis of Besnoitia besnoiti. infection.
    Vet Parasitol. 178 (3-4): 217-22.
  5. Grant, C.F. et al. (2012) Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
    Vet Res. 43: 68.
  6. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.
    Clin Vaccine Immunol. 20: 1669-74.
  7. Vrieling, M. et al. (2013) Hsp70 vaccination-induced primary immune responses in efferent lymph of the draining lymph node.
    Vaccine. 31 (42): 4720-7.
  8. Somda, M.B. et al. (2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.
    Vet Parasitol. 197 (1-2): 332-40.
    9. View The Latest Product References
  9. Hosking, C.G. et al. (2015) Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum.
    Int J Parasitol. 45 (11): 729-40.
  10. Subharat, S. et al. (2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.
    Vet Immunol Immunopathol. 164 (3-4): 201-7.
  11. Facciuolo, A. et al. (2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
    PLoS One. 11 (7): e0158747.
  12. Somda, M.B. et al. (2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
    Parasit Vectors. 9 (1): 149.
  13. Benedictus, L. et al. (2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.
    Vaccine. 34 (8): 1002-5.
  14. Denholm, S.J. et al. (2018) Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements.
    J Dairy Sci. 101 (11): 10248-10258.
  15. Alo, K et al. (2018) Passive protective effect of anti-K99 antibodies against enterotoxigenic E.coli. infection in neonatal calves
    Ir J of Vet Med, 12(2), 97-107
  16. Springer, A. et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
    Pathogens 2022, 11, 55.
  17. Anastácio, C. et al. (2022) Impact of Endemic Besnoitiosis on the Performance of a Dairy Cattle Herd.
    Animals (Basel). 12 (10): 1291.

RRID
AB_323065

AAI23

148580 153249 159609 165841

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