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IgG antibody

Sheep anti Bovine IgG

Product Type
Polyclonal Antibody
Polyclonal IgG

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ID 1 mg loader
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Sheep anti Bovine IgG polyclonal antibody recognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other species

Sheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrieling et al. 2013).

Target Species
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Purified bovine IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
Description Rabbit anti Sheep IgG (H/L):Biotin

References for IgG antibody

  1. Naylor, S.W. et al. (2007) Impact of the direct application of therapeutic agents to the terminal recta of experimentally colonized calves on Escherichia coli O157:H7 shedding.
    Appl Environ Microbiol. 73: 1493-500.
  2. Cortes, H.C. et al. (2007) Application of conventional and real-time fluorescent ITS1 rDNA PCR for detection of Besnoitia besnoiti. infections in bovine skin biopsies.
    Vet Parasitol. 146 (3-4): 352-6.
  3. Bridger, P.S. et al. (2011) Detection of colostrum-derived alloantibodies in calves with bovine neonatal pancytopenia.
    Vet Immunol Immunopathol. 141: 1-10.
  4. Waap, H. et al. (2011) A modified agglutination test for the diagnosis of Besnoitia besnoiti. infection.
    Vet Parasitol. 178 (3-4): 217-22.
  5. Grant, C.F. et al. (2012) Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
    Vet Res. 43: 68.
  6. Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.
    Clin Vaccine Immunol. 20: 1669-74.
  7. Vrieling, M. et al. (2013) Hsp70 vaccination-induced primary immune responses in efferent lymph of the draining lymph node.
    Vaccine. 31 (42): 4720-7.
  8. Somda, M.B. et al. (2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.
    Vet Parasitol. 197 (1-2): 332-40.
  9. View The Latest Product References
  10. Hosking, C.G. et al. (2015) Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum.
    Int J Parasitol. 45 (11): 729-40.
  11. Subharat, S. et al. (2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.
    Vet Immunol Immunopathol. 164 (3-4): 201-7.
  12. Facciuolo, A. et al. (2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
    PLoS One. 11 (7): e0158747.
  13. Somda, M.B. et al. (2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
    Parasit Vectors. 9 (1): 149.
  14. Benedictus, L. et al. (2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.
    Vaccine. 34 (8): 1002-5.
  15. Denholm, S.J. et al. (2018) Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements.
    J Dairy Sci. 101 (11): 10248-10258.
  16. Alo, K et al. (2018) Passive protective effect of anti-K99 antibodies against enterotoxigenic E.coli. infection in neonatal calves
    Ir J of Vet Med, 12(2), 97-107
  17. Springer, A. et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
    Pathogens 2022, 11, 55.
  18. Anastácio, C. et al. (2022) Impact of Endemic Besnoitiosis on the Performance of a Dairy Cattle Herd.
    Animals (Basel). 12 (10): 1291.
  19. Coelho, J. et al. (2023) Epidemiological characteristics of bovine besnoitiosis (Besnoitia besnoiti) in a beef cattle farm: a cross-sectional serological assessment.
    Front Vet Sci. 10: 1158235.
  20. Salles, M.S.V. et al. (2024) Supplementation of essential nutrients, selenium, iron and vitamin E for suckling calves under immunological challenge
    Res Sq 12 Apr [Preprint]



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