IgG antibody






Sheep anti Bovine IgG:Alk. Phos.
Sheep anti Bovine IgG:Biotin
Sheep anti Bovine IgG:FITC
Sheep anti Bovine IgG:HRP
Sheep anti Bovine IgG
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
AAI23AB | E WB | 0.5 mg |
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AAI23B | E WB | 1 mg |
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AAI23F | C F | 1 mg |
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AAI23P | E WB | 1 mg |
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AAI23 | E ID | 1 mg |
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Sheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrieling et al. 2013).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG fraction conjugated to Biotin - liquid
- Product Form
- Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.05% Proclin™ 300
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified bovine IgG.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/5000 | 1/50000 | |
Western Blotting | 1/5000 | 1/50000 | |
ELISA | 1:10000 | 1:100000 | |
Western Blotting | 1:10000 | 1:100000 | |
Flow Cytometry | |||
Immunohistology - Frozen | 1/200 | 1/2,000 | |
ELISA | 1/10,000 | 1/100,000 | |
Western Blotting | |||
ELISA | 1/100 | 1/30,000 | |
Immunodiffusion |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for IgG antibody
-
Santema, W.J. (1982) Hsp70 as a candidate subunit vaccine for paratuberculosis
Dissertation, University of Utrecht. -
Duncombe, L. et al. (2013) Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon.
Clin Vaccine Immunol. 20: 1669-74. -
Vrieling, M. et al. (2013) Hsp70 vaccination-induced primary immune responses in efferent lymph of the draining lymph node.
Vaccine. 31 (42): 4720-7. -
Bridger, P.S. et al. (2011) Detection of colostrum-derived alloantibodies in calves with bovine neonatal pancytopenia.
Vet Immunol Immunopathol. 141: 1-10. -
Grant, C.F. et al. (2012) Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
Vet Res. 43: 68. -
Naylor, S.W. et al. (2007) Impact of the direct application of therapeutic agents to the terminal recta of experimentally colonized calves on Escherichia coli O157:H7 shedding.
Appl Environ Microbiol. 73: 1493-500. -
Somda, M.B. et al. (2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.
Vet Parasitol. 197 (1-2): 332-40. -
Hosking, C.G. et al. (2015) Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum.
Int J Parasitol. 45 (11): 729-40. -
Somda, M.B. et al. (2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
Parasit Vectors. 9 (1): 149. -
Facciuolo, A. et al. (2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
PLoS One. 11 (7): e0158747. -
Subharat, S. et al. (2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.
Vet Immunol Immunopathol. 164 (3-4): 201-7. -
Benedictus, L. et al. (2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.
Vaccine. 34 (8): 1002-5. -
Denholm, S.J. et al. (2018) Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements.
J Dairy Sci. 101 (11): 10248-10258.
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