GAP43 antibody

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AHP669
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C IF IP P WB 0.1 ml loader
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Rabbit anti GAP43 antibody recognises the protein designated GAP43, a neuronal protein that is expressed at elevated levels during development and also during regenerative axon growth.

GAP43 (also known as neuromodulin, pp46, B-50, Protein F1 and calmodulin-binding protein P-57) is a protein of approximately 25 kDa that migrates on SDS-PAGE gels with an apparent size of 40-50 kDa. The aberrant migration is due to the unusually low abundance of hydrophobic amino acid residues. GAP43 is found tightly associated with neuronal membranes due to palmitoylation. The solubilised protein binds calmodulin, although this is reduced following protein kinase C phosphorylation of GAP43.

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Monkey
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to GAP43 were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified GAP43 from cat brain.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for GAP43 antibody

  1. McIntosh, H. et al. (1989) A GAP-43-like protein in cat visual cortex.
    Vis Neurosci. 2 (6): 583-91.
  2. Wang, H. et al. (2009) Neuroprotective effect of recombinant human erythropoietin on optic nerve injury in rats.
    Chin Med J 122: 2008-12.
  3. Zhang, H. et al. (2010) Alteration of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation modified electrophysiological function of heart: a novel antiarrhythmic therapy.
    Circulation. 122 (11 Suppl): S193-200.
  4. Parikh, P. et al. (2011) Regeneration of axons in injured spinal cord by activation of bone morphogenetic protein/Smad1 signaling pathway in adult neurons.
    Proc Natl Acad Sci U S A. 108: E99-107.
  5. Turner, J.H. et al. (2010) Reversible loss of neuronal marker protein expression in a transgenic mouse model for sinusitis-associated olfactory dysfunction.
    Am J Rhinol Allergy. 24: 192-6.
  6. Su, Y. et al. (2008) Axonal regeneration after optic nerve crush in Nogo-A/B/C knockout mice.
    Mol Vis. 14: 268-73.
  7. Pao, P.C. et al. (2011) A novel RING finger protein, Znf179, modulates cell cycle exit and neuronal differentiation of P19 embryonal carcinoma cells.
    Cell Death Differ. 18 (11): 1791-804.
  8. Cheng, H.T. et al. (2013) Increased Axonal Regeneration and Swellings in Intraepidermal Nerve Fibers Characterize Painful Phenotypes of Diabetic Neuropathy.
    J Pain. pii: S1526-5900(13)00910-3.
  9. View The Latest Product References
  10. Wu, X. et al. (2012) Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
    J Biomed Biotechnol. 2012: 732909.
  11. Oberbauer, E. et al. (2013) Chroman-like cyclic prenylflavonoids promote neuronal differentiation and neurite outgrowth and are neuroprotective.
    J Nutr Biochem. 24 (11): 1953-62.
  12. Su, Y. et al. (2017) Transfection with AAV-NgR1siRNA on axonal regeneration of optic nerve after injury
    Int J Clin Exp Pathol. 10 (2) :2327-32.

Immunofluorescence

Immunohistology - Frozen

Western Blotting

RRID
AB_2247457
UniProt
Q6S9D9
Entrez Gene
GAP43
GO Terms
GO:0005516 calmodulin binding
GO:0030054 cell junction
GO:0032584 growth cone membrane
GO:0040008 regulation of growth
GO:0045202 synapse
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