GAP43 antibody
Rabbit anti GAP43 antibody (AHP669) used to demonstrate GAP43 expression in nerve fibers in a rat myocardial ischemia model by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
GAP43 immunohistochemical staining of the infarct border zone (DAB color, ×200). (a) Sham-operated group. The distribution of GAP43-positive nerve fibers is orderly. (b) Control ischemic group. GAP43-positive nerve fibers in ventricular muscle increased significantly, it is also thickened and disordered. (c) Preischemic treatment group. (d) Delayed application group. The distribution of GAP43-positive nerve fibers after treatment with desipramine significantly improved.
From: Wu X, Jiang H, Yu L, Hu X, Liu W.
Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
J Biomed Biotechnol. 2012;2012:732909.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
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P ResetRabbit anti GAP43
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- GAP43
Antibody Quality and Performance Guarantee
| Rabbit anti GAP43 antibody recognises the protein designated GAP43, a neuronal protein that is expressed at elevated levels during development and also during regenerative axon growth. GAP43 (also known as neuromodulin, pp46, B-50, Protein F1 and calmodulin-binding protein P-57) is a protein of approximately 25 kDa that migrates on SDS-PAGE gels with an apparent size of 40-50 kDa. The aberrant migration is due to the unusually low abundance of hydrophobic amino acid residues. GAP43 is found tightly associated with neuronal membranes due to palmitoylation. The solubilised protein binds calmodulin, although this is reduced following protein kinase C phosphorylation of GAP43. |
- Target Species
- Cat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat Monkey Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to GAP43 were raised by repeated immunisations of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified GAP43 from cat brain.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
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| Western Blotting | |
1/500 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for GAP43 antibody
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McIntosh, H. et al. (1989) A GAP-43-like protein in cat visual cortex.
Vis Neurosci. 2 (6): 583-91. -
Su, Y. et al. (2008) Axonal regeneration after optic nerve crush in Nogo-A/B/C knockout mice.
Mol Vis. 14: 268-73. -
Wang, H. et al. (2009) Neuroprotective effect of recombinant human erythropoietin on optic nerve injury in rats.
Chin Med J 122: 2008-12. -
Turner, J.H. et al. (2010) Reversible loss of neuronal marker protein expression in a transgenic mouse model for sinusitis-associated olfactory dysfunction.
Am J Rhinol Allergy. 24: 192-6. -
Zhang, H. et al. (2010) Alteration of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation modified electrophysiological function of heart: a novel antiarrhythmic therapy.
Circulation. 122 (11 Suppl): S193-200. -
Pao, P.C. et al. (2011) A novel RING finger protein, Znf179, modulates cell cycle exit and neuronal differentiation of P19 embryonal carcinoma cells.
Cell Death Differ. 18 (11): 1791-804. -
Parikh, P. et al. (2011) Regeneration of axons in injured spinal cord by activation of bone morphogenetic protein/Smad1 signaling pathway in adult neurons.
Proc Natl Acad Sci U S A. 108 (19): E99-107. -
Wu, X. et al. (2012) Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
J Biomed Biotechnol. 2012: 732909.
View The Latest Product References
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Oberbauer, E. et al. (2013) Chroman-like cyclic prenylflavonoids promote neuronal differentiation and neurite outgrowth and are neuroprotective.
J Nutr Biochem. 24 (11): 1953-62. -
Cheng, H.T. et al. (2013) Increased axonal regeneration and swellings in intraepidermal nerve fibers characterize painful phenotypes of diabetic neuropathy.
J Pain. 14 (9): 941-7. -
Su, Y. et al. (2017) Transfection with AAV-NgR1siRNA on axonal regeneration of optic nerve after injury
Int J Clin Exp Pathol. 10 (2) :2327-32.
- RRID
- AB_2247457
- UniProt
- Q6S9D9
- Entrez Gene
- GAP43
- GO Terms
- GO:0005516 calmodulin binding
- GO:0030054 cell junction
- GO:0032584 growth cone membrane
- GO:0040008 regulation of growth
- GO:0045202 synapse
AHP669
151341
157544
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