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Escherichia coli J5 LPS antibody

Sheep anti Escherichia coli J5 LPS

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Escherichia coli J5 LPS

Product Code Applications Pack Size List Price Your Price Qty
5684-1000
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 ml loader
List Price Your Price
loader

Sheep anti Escherichia coli antibody recognizes the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

Target Species
Bacterial
Product Form
Serum - liquid
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Heat inactivated Escherichia coli J5.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
loader
Description Rabbit anti Sheep IgG (H/L):Biotin

RRID
AB_619719

5684-1000

153867 159894 162677

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