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Escherichia coli antibody

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Anti Escherichia coli Antibody gallery image 1
Published customer image:
AFM scan of E. coli K-12 specifically bound on the cover slip modified by APTES and antibody Bio-Rad 4329-4906. The “Error” signal is shown. (A) Overview and (B) detail of a single cell.

From: Farka, Z et al.Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors 2015, 15, 79-92.

Rabbit anti Escherichia coli:Biotin

Rabbit anti Escherichia coli:FITC

Rabbit anti Escherichia coli

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
4329-4911
Datasheet
E
MSDS
1 ml loader

4329-4916
Datasheet
IF
MSDS
1 ml loader

4329-4906
Datasheet
E
MSDS
1 ml loader

Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide.

This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12.

Product Details

Target Species
Bacterial
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
A mixture of all antigenic serotypes.
Approx. Protein Concentrations
4.0 mg/ml
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Escherichia coli antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence 1/10 1/50
ELISA 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

References for Escherichia coli antibody

  1. Tian, B. et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
    Biosens Bioelectron. 77: 32-9.
  2. Skladal, P. et al. (2013) Electrochemical immunosensors for detection of microorganisms
    Int. J. Electrochem. Sci., 8 1635 - 49
  3. Su, W.H. et al. (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections.
    Taiwan J Obstet Gynecol. 52: 25-32.
  4. Ho, T.Y. et al. (2013) Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin.
    Biosens Bioelectron. 49: 126-32.
  5. Ong, L.C. et al. (2014) Bacterial imaging with photostable upconversion fluorescent nanoparticles.
    Biomaterials. 35 (9): 2987-98.
  6. Pivetal, J. et al. (2014) Micro-magnet arrays for specific single bacterial cell positioning
    J. Magnetism and magnetic Materials 29 Sep [Epub ahead of print]
  7. Farka, Z. et al. (2015) Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
    Sensors 15, 79-92
  8. Dayam RM et al. (2015) The Phosphoinositide-Gated Lysosomal Ca(2+) Channel, TRPML1, Is Required for Phagosome Maturation.
    Traffic. 16 (9): 1010-26.
  9. Farka, Z. et al. (2015) Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms.
    Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, 945507
  10. Bhokisham, N. et al. (2016) Modular Construction of Multi-Subunit Protein Complexes using Engineered Tags and Microbial Transglutaminase.
    Metab Eng. May 26. pii: S1096-7176(16)30014-3. [Epub ahead of print]
  11. Kovář, D. et al. (2014) Detection of aerosolized biological agents using the piezoelectric immunosensor.
    Anal Chem. 86 (17): 8680-6.
  12. VanGerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
    Mol Microbiol. 91 (5): 1022-35.
  13. Rodrigues, D.M.C. et al. (2017) Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results.
    Sensors (Basel). 17 (12) Dec 19 [Epub ahead of print].
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