Escherichia coli antibody

Rabbit anti Escherichia coli

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Escherichia coli

Product Code Applications Pack Size List Price Your Price Qty
4329-4906
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SDS Safety Datasheet SDS
E 1 ml loader
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Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide.

This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12.

Target Species
Bacterial
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
A mixture of all antigenic serotypes.
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Escherichia coli antibody

  1. Tian, B. et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
    Biosens Bioelectron. 77: 32-9.
  2. Skladal, P. et al. (2013) Electrochemical immunosensors for detection of microorganisms
    Int. J. Electrochem. Sci., 8 1635 - 49
  3. Su, W.H. et al. (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections.
    Taiwan J Obstet Gynecol. 52: 25-32.
  4. Ho, T.Y. et al. (2013) Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin.
    Biosens Bioelectron. 49: 126-32.
  5. Ong, L.C. et al. (2014) Bacterial imaging with photostable upconversion fluorescent nanoparticles.
    Biomaterials. 35 (9): 2987-98.
  6. Pivetal, J. et al. (2014) Micro-magnet arrays for specific single bacterial cell positioning
    J. Magnetism and magnetic Materials 29 Sep [Epub ahead of print]
  7. Farka, Z. et al. (2015) Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
    Sensors 15, 79-92
  8. Dayam RM et al. (2015) The Phosphoinositide-Gated Lysosomal Ca(2+) Channel, TRPML1, Is Required for Phagosome Maturation.
    Traffic. 16 (9): 1010-26.
  9. View The Latest Product References
  10. Farka, Z. et al. (2015) Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms.
    Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, 945507
  11. Bhokisham, N. et al. (2016) Modular Construction of Multi-Subunit Protein Complexes using Engineered Tags and Microbial Transglutaminase.
    Metab Eng. May 26. pii: S1096-7176(16)30014-3. [Epub ahead of print]
  12. Kovář, D. et al. (2014) Detection of aerosolized biological agents using the piezoelectric immunosensor.
    Anal Chem. 86 (17): 8680-6.
  13. VanGerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
    Mol Microbiol. 91 (5): 1022-35.
  14. Rodrigues, D.M.C. et al. (2017) Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results.
    Sensors (Basel). 17 (12) Dec 19 [Epub ahead of print].

RRID
AB_619412
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