Escherichia coli antibody
AFM scan of E. coli K-12 specifically bound on the cover slip modified by APTES and antibody Bio-Rad 4329-4906. The “Error” signal is shown. (A) Overview and (B) detail of a single cell.
From: Farka, Z et al.Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors 2015, 15, 79-92.
Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide.
This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12.
This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- A mixture of all antigenic serotypes.
- Approx. Protein Concentrations
- 4.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Escherichia coli antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | 1/10 | 1/50 | |
| ELISA | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Product Specific References
References for Escherichia coli antibody
-
Tian, B. et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
Biosens Bioelectron. 77: 32-9. -
Skladal, P. et al. (2013) Electrochemical immunosensors for detection of microorganisms
Int. J. Electrochem. Sci., 8 1635 - 49 -
Su, W.H. et al. (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections.
Taiwan J Obstet Gynecol. 52: 25-32. -
Ho, T.Y. et al. (2013) Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin.
Biosens Bioelectron. 49: 126-32. -
Ong, L.C. et al. (2014) Bacterial imaging with photostable upconversion fluorescent nanoparticles.
Biomaterials. 35 (9): 2987-98. -
Pivetal, J. et al. (2014) Micro-magnet arrays for specific single bacterial cell positioning
J. Magnetism and magnetic Materials 29 Sep [Epub ahead of print] -
Farka, Z. et al. (2015) Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
Sensors 15, 79-92 -
Dayam RM et al. (2015) The Phosphoinositide-Gated Lysosomal Ca(2+) Channel, TRPML1, Is Required for Phagosome Maturation.
Traffic. 16 (9): 1010-26. -
Farka, Z. et al. (2015) Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms.
Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, 945507 -
Bhokisham, N. et al. (2016) Modular Construction of Multi-Subunit Protein Complexes using Engineered Tags and Microbial Transglutaminase.
Metab Eng. May 26. pii: S1096-7176(16)30014-3. [Epub ahead of print] -
Kovář, D. et al. (2014) Detection of aerosolized biological agents using the piezoelectric immunosensor.
Anal Chem. 86 (17): 8680-6. -
VanGerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
Mol Microbiol. 91 (5): 1022-35. -
Rodrigues, D.M.C. et al. (2017) Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results.
Sensors (Basel). 17 (12) Dec 19 [Epub ahead of print].
