Escherichia coli antibody
Rabbit anti Escherichia coli. antibody (4329-4906 used to label bacterium by atomic force microscopy.
Image caption:
AFM scan of E. coli K-12 specifically bound on the cover slip modified by APTES and antibody Bio-Rad 4329-4906. The “Error” signal is shown. (A) Overview and (B) detail of a single cell.
From: Farka, Z et al.Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors 2015, 15, 79-92.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Escherichia coli. antibody (4329-4906) used as an antibody reagent in an E. coli biosensor.
Image caption:
(A) Testing the binding abilities of different polyclonal Abs with an in-house ELISA test with different amounts of intact E. coli cells as antigen. Black: Bio-Rad 4329-4906, white: Thermo Fisher PA1-7213, grey: Thermo Fisher PA1-73032. (B) The binding ability of a monoclonal Ab (Bio-Rad OBT0749) with an in-house ELISA test with different amounts of intact E. coli cells as antigen. For detection, biotinylated polyclonal Ab (Bio-Rad 4329-4906) was used. The inset shows the schematic drawing of the assay.
From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Escherichia coli. antibody (4329-4906) used as an antibody reagent in an E. coli biosensor.
Image caption:
Typical OWLS kinetic curves of Ab deposition and their corresponding fits are shown. (A) Polyclonal Ab (Bio-Rad rabbit 4329-4906 on protein A surface). (B) Monoclonal Ab (Bio-Rad mouse OBT0749 on protein A surface). The smaller adsorbed surface mass density and slower adsorption are clearly visible in the case of the monoclonal Ab.
From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Escherichia coli. antibody (4329-4906) used as an antibody reagent in an E. coli biosensor.
Image caption:
Bacterial adsorption on the Ab-coated sensor surfaces and the detection limit of OWLS. (A) Sensogram of a complete experiment, including the in situ coating procedures and subsequent bacteria adsorption for protein A-based immobilization and PAcrAM-P blocking. (B) E. coli adsorption on polyclonal Ab-coated surfaces using PAcrAM-P blocking; a 109 cells/mL concentration was employed. The signals with statistics are clearly distinguishable from the relevant control signal (full layer without Ab). (C) OWLS signal for a series of bacterial concentrations using protein A-based immobilization with PAcrAM-P blocking.From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
AFM BS ResetRabbit anti Escherichia coli
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Escherichia coli
Antibody Quality and 
Unrivaled 
| Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide. This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- A mixture of all antigenic serotypes.
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Escherichia coli antibody
-
Tian, B. et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
Biosens Bioelectron. 77: 32-9. -
Skladal, P. et al. (2013) Electrochemical immunosensors for detection of microorganisms
Int. J. Electrochem. Sci., 8 1635 - 49 -
Su, W.H. et al. (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections.
Taiwan J Obstet Gynecol. 52: 25-32. -
Ho, T.Y. et al. (2013) Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin.
Biosens Bioelectron. 49: 126-32. -
Ong, L.C. et al. (2014) Bacterial imaging with photostable upconversion fluorescent nanoparticles.
Biomaterials. 35 (9): 2987-98. -
Pivetal, J. et al. (2014) Micro-magnet arrays for specific single bacterial cell positioning
J. Magnetism and magnetic Materials 29 Sep [Epub ahead of print] -
Farka, Z. et al. (2015) Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
Sensors 15, 79-92 -
Dayam RM et al. (2015) The Phosphoinositide-Gated Lysosomal Ca(2+) Channel, TRPML1, Is Required for Phagosome Maturation.
Traffic. 16 (9): 1010-26.
View The Latest Product References
-
Farka, Z. et al. (2015) Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms.
Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, 945507 -
Bhokisham, N. et al. (2016) Modular Construction of Multi-Subunit Protein Complexes using Engineered Tags and Microbial Transglutaminase.
Metab Eng. May 26. pii: S1096-7176(16)30014-3. [Epub ahead of print] -
Kovář, D. et al. (2014) Detection of aerosolized biological agents using the piezoelectric immunosensor.
Anal Chem. 86 (17): 8680-6. -
VanGerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
Mol Microbiol. 91 (5): 1022-35. -
Rodrigues, D.M.C. et al. (2017) Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results.
Sensors (Basel). 17 (12) Dec 19 [Epub ahead of print].
- RRID
- AB_619412
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