Beta Galactosidase antibody

Rabbit anti Beta Galactosidase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Beta Galactosidase

Product Code Applications Pack Size List Price Your Price Qty
AHP1292GA
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C E P WB 0.1 mg loader
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Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccharides.

Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.

Target Species
Bacterial
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Beta-galactosidase prepared from E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/400 1/1600
Immunohistology - Frozen 1/50 1/300
Immunohistology - Paraffin 1/50 1/300
Western Blotting 1/200 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1292GA detects a band of approximately 117kDa using E. coli beta-galactosidase.

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References for Beta Galactosidase antibody

  1. Nam, K.T. et al. (2012) Spasmolytic polypeptide-expressing metaplasia (SPEM) in the gastric oxyntic mucosa does not arise from Lgr5-expressing cells.
    Gut. 61 (12): 1678-85.
  2. Wende, C.Z. et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.
    PLoS One. 10 (5): e0127681.

Further Reading

  1. Matthews, B.W. (2005) The structure of E. coli beta-galactosidase.
    C R Biol. 328 (6): 549-56.

RRID
AB_10571346
UniProt
P00722
GO Terms
GO:0005515 protein binding
GO:0004565 beta-galactosidase activity
GO:0030246 carbohydrate binding
GO:0046872 metal ion binding
GO:0005990 lactose catabolic process
GO:0009341 beta-galactosidase complex