Beta Galactosidase antibody

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Rabbit anti Beta Galactosidase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1292GA C E P WB 0.1 mg
Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccharides.

Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.

Product Details

Target Species
Bacterial
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Beta-galactosidase prepared from E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P00722 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004565 beta-galactosidase activity
GO:0030246 carbohydrate binding
GO:0046872 metal ion binding
GO:0005990 lactose catabolic process
GO:0009341 beta-galactosidase complex
Regulatory
For research purposes only

Applications of Beta Galactosidase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/400 1/1600
Immunohistology - Frozen 1/50 1/300
Immunohistology - Paraffin 1/50 1/300
Western Blotting 1/200 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1292GA detects a band of approximately 117kDa using E. coli beta-galactosidase.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Beta Galactosidase antibody

  1. Nam, K.T. et al. (2012) Spasmolytic polypeptide-expressing metaplasia (SPEM) in the gastric oxyntic mucosa does not arise from Lgr5-expressing cells.
    Gut. 61 (12): 1678-85.
  2. Wende, C.Z. et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.
    PLoS One. 10 (5): e0127681.

Further Reading

  1. Matthews, B. W. (2005) The structure of E. coli beta-galactosidase.
    C. R. Biol. 328: 549-556.