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Amyloid Precursor Protein antibody

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Anti Amyloid Precursor Protein (C-Terminal) Antibody thumbnail image 1 Anti Amyloid Precursor Protein (C-Terminal) Antibody thumbnail image 2 Anti Amyloid Precursor Protein (C-Terminal) Antibody thumbnail image 3 Anti Amyloid Precursor Protein (C-Terminal) Antibody thumbnail image 4
Anti Amyloid Precursor Protein (C-Terminal) Antibody gallery image 1
Western blot analysis of APP in mouse (M) and rat (R) brain tissue lysates probed with Rabbit anti amyloid precursor protein antibody (AHP538).
Anti Amyloid Precursor Protein (C-Terminal) Antibody gallery image 2
Immunohistochemical staining of rat brain with Rabbit anti amyloid precursor protein antibody (AHP538).
Anti Amyloid Precursor Protein (C-Terminal) Antibody gallery image 3
Published customer image:
Rabbit anti Amyloid precursor protein antibody used to detect the amyloid β CT fragment by western blotting.
Image caption:
Western blotting analysis result of BACE1 and β-CTF. A: bands of BACE1, β-CTF and β-tubulin; B: optical density analysis of BACE1; C: optical density analysis of β-CTF. *: compared with normal control group: p<0.05; #: compared with vehicle control group: p>0.05; Δ: compared with normal control group: p<0.05; **: compared with model groups or vehicle control group: p<0.05, n=3.

From: Huang JF, Shang L, Liu P, Zhang MQ, Chen S, Chen D, Fan CL, Wang H, Xiong K. Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina.
BMC Complement Altern Med. 2012 Oct 22;12:189.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Amyloid Precursor Protein (C-Terminal) Antibody gallery image 4
Published customer image:
Rabbit anti Amyloid precursor protein antibody (AHP538) used for the detection of the amyloid C99 fragment by western blotting of leptomeningeal lysates
Image caption:
Characterization of BACE1 elevation in meningeal cells associated with leptomeningeal amyloidosis. Panel (A) shows BACE1 labeled cells aligning along the pia mater in a case with cerebral amyloid angiopathy with prominent leptomeningeal amyloidosis (B), in contrast to a control case wherein no BACE1 and Aβ (6E10) labeling is present at the pia (C,D). Arrows in (A,B) points to profiles with increased BACE1 and 6E10 IR over the background. Panel (E) shows a preparation of leptomeninge sample from the temporal lobe (E), which are rinsed thoroughly in cold phosphate buffer for biochemical analysis (F). Levels of BACE1 protein and C99 in leptomeningeal lysates are elevated in 5 cases with CAA relative to 5 control cases (G,H), as is BACE1 enzymatic activity measured in the lysates (H). Aβ42 levels are also higher in the CAA group relative to control (H). In primary cell culture of leptomeningeal biopsies, meningeal cells appear polygonal under phase contrast microscope (J), co-express fibronectin (FN) (K) and BACE1 (L). These cells also express the β-amyloid precursor protein (APP) (H, the corresponding FN labeling image is not shown). Scale bar = 250 μm in (A) applying to (B-D), equivalent to 50 μm for inserts in (A,B), 25 μm for (J) and 12.5 μm for (K-M).

From: Xue ZQ, He ZW, Yu JJ, Cai Y, Qiu WY, Pan A, Gai WP, Cai H, Luo XG, Ma C, Yan XX.
Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal β-amyloid deposition in the human brain.
BMC Neurol. 2015 May 2;15:71.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Rabbit anti Amyloid Precursor Protein (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Amyloid Precursor Protein
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP538
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Amyloid Precursor Protein antibody recognizes both intact Amyloid Precursor Protein (APP), and also the C99 fragment generated by beta-secretase. The sequence recognized by this antibody corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kDa beta amyloid peptide, found in the brains of Alzheimer's disease patients.

APP also inhibits Notch signaling through it’s interaction with NUMB (Roncarati et al. 2002). Rabbit anti Amyloid Precursor Protein antibody has been used successfully for the detection of the ~14 kDa β C-terminal fragment of APP, produced as a result of cleavage by BACE1 using western blotting -in rat retinal lysates (Huang et al.2012).

Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to APP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 737-751 of human APP.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/500 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin section
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Amyloid Precursor Protein antibody

  1. Yan, X.X. et al. (2007) beta-Secretase expression in normal and functionally deprived rat olfactory bulbs: inverse correlation with oxidative metabolic activity.
    J Comp Neurol. 501: 52-69.
  2. Zhang, H. et al. (2011) IGF-1 reduces BACE-1 expression in PC12 cells via activation of PI3-K/Akt and MAPK/ERK1/2 signaling pathways.
    Neurochem Res. 36: 49-57.
  3. Désiré, L. et al. (2005) RAC1 inhibition targets amyloid precursor protein processing by gamma-secretase and decreases Abeta production in vitro and in vivo.
    J Biol Chem. 280: 37516-25.
  4. Huang JF et al. (2012) Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina.
    BMC Complement Altern Med. 12: 189.
  5. Xiong, K. et al. (2007) Mitochondrial respiratory inhibition and oxidative stress elevate beta-secretase (BACE1) proteins and activity in vivo in the rat retina.
    Exp Brain Res. 181: 435-46.
  6. Zhang, X.M. et al. (2010) Functional deprivation promotes amyloid plaque pathogenesis in Tg2576 mouse olfactory bulb and piriform cortex.
    Eur J Neurosci. 31: 710-21.
  7. Cai, Y. et al. (2010) β-Secretase-1 elevation in aged monkey and Alzheimer's disease human cerebral cortex occurs around the vasculature in partnership with multisystem axon terminal pathogenesis and β-amyloid accumulation.
    Eur J Neurosci. 32: 1223-38.
  8. Marcade, M. et al. (2008) Etazolate, a neuroprotective drug linking GABA(A) receptor pharmacology to amyloid precursor protein processing.
    J Neurochem. 106: 392-404.
    9. View The Latest Product References
  9. Cai, Y. et al. (2012) BACE1 elevation is involved in amyloid plaque development in the triple transgenic model of Alzheimer's disease: differential Aβ antibody labeling of early-onset axon terminal pathology.
    Neurotox Res. 21: 160-74.
  10. Zhang, H. et al. (2015) Hydrogen sulfide-induced processing of the amyloid precursor protein in SH-SY5Y human neuroblastoma cells involves the PI3-K/Akt signaling pathway.
    Cell Mol Neurobiol. 35 (2): 265-72.
  11. Xue, Z.Q. et al. (2015) Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal β-amyloid deposition in the human brain.
    BMC Neurol. 15: 71.
  12. Belichenko, P.V. et al. (2016) An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down Syndrome.
    PLoS One. 11 (3): e0152471.
  13. He, X.L. et al. (2016) Hydrogen sulfide down-regulates BACE1 and PS1 via activating PI3K/Akt pathway in the brain of APP/PS1 transgenic mouse.
    Pharmacol Rep. 68 (5): 975-82.

Further Reading

  1. Ponte, P. et al. (1988) A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors.
    Nature 331: 525-527.
  2. Selkoe, D.J. (1994) Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease.
    Annu Rev Cell Biol. 10: 373-403.

Western Blotting

Synonyms
APP
RRID
AB_321972
UniProt
P05067
Entrez Gene
APP

AHP538

148508 158578 160034 162787

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