Acetylcholine antibody

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Rabbit anti Acetylcholine

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Acetylcholine

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Secondary Antibodies
References (3)
Applications
Storage Information

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0030-2004 Datasheet Datasheet SDS SDS	C E	1 ml		Log in	Get Quote
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Rabbit anti acetylcholine polyclonal antibody recognizes the small cationic neurotransmitter acetylcholine, involved in the transmission of nerve impulses across synapses. The structure of acetylcholine is preserved across the animal kingdom and the Rabbit anti acetylcholine is expected to recognize acetylcholine from all species posessing this neurotransmitter.

Target Species: Broad
Species Cross-Reactivity: Target Species Cross Reactivity

Rat

Human; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified Ig - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Synthetic acetylcholine conjugated to Keyhole limpet Haemocyanin.
Approx. Protein Concentrations: Ig concentration 5.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Rat
Human

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/50	1/500
Immunohistology - Frozen

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Histology Positive Control Tissue: Rat brain tissue

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References for Acetylcholine antibody

Amenta, F. et al. (2006) Association with the cholinergic precursor choline alphoscerate and the cholinesterase inhibitor rivastigmine: an approach for enhancing cholinergic neurotransmission.
Mech Ageing Dev. 127: 173-9.
El-Assouad, D. and Tayebati, S.K. (2002) Cholinergic innervation of pial arteries in senescent rats: an immunohistochemical study.
Mech Ageing Dev. 123: 529-36.
Tayebati, S.K. et al. (2002) Immunochemical and immunocytochemical characterization of cholinergic markers in human peripheral blood lymphocytes.
J Neuroimmunol. 132: 147-55.