CD27 Antibody | B30C7

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CD27 Antibody | B30C7 gallery image 1

Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:FITC (MCA5973F) and Mouse anti Pig wCD8a:RPE (MCA1223PE)

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CD27 Antibody | B30C7 gallery image 2

Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27 (MCA5973GA) detected with Goat anti Mouse IgG:FITC (STAR117F), and Mouse anti Pig wCD8a:RPE (MCA1223PE)

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CD27 Antibody | B30C7 gallery image 3

Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:APC (MCA5973APC) and Mouse anti Pig wCD8a:RPE (MCA1223PE)

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CD27 Antibody | B30C7 gallery image 4

Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD27:RPE (MCA5973PE) and Mouse anti Pig wCD8a:FITC (MCA1223F)

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CD27 Antibody | B30C7 gallery image 5

Figure A. FITC conjugated mouse anti porcine CD18a (MCA1972F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD18a (MCA1972F) and RPE conjugated mouse anti porcine CD27 (MCA5973PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD27 Antibody | B30C7 gallery image 6

Figure A. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated mouse anti porcine CD27 (MCA5973PE) and FITC conjugated mouse anti porcine CD18a (MCA1972F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD27:FITC
  • Mouse anti Pig CD27:RPE
  • Mouse anti Pig CD27:APC
  • Mouse anti Pig CD27
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  • Product Type
    Monoclonal Antibody
  • Clone
    B30C7
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5973FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5973F
    MCA5973GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA5973GA
    MCA5973PEFdatasheet pdfdatasheet pdf100 Tests
    MCA5973PE
    MCA5973APCFdatasheet pdfdatasheet pdf100 Tests
    MCA5973APC
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however studies undertaken using the B30C7 clone have been unable to detect expression of CD27 on porcine B-cells (Reutner et al. 2012).

      Studies in humans have demonstrated CD27 to be involved in apoptosis, research in swine has shown that porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells (Reutner et al. 2012).

      Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and research utilizing clone B30C7 has shown that this monoclonal antibody may be used to differentiate between subsets of pig NK cells (Mair et al. 2013).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine peripheral blood monocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0-Ag14 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      12 months from date of reconstitution
      12 months from date of reconstitution
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD27 Antibody Formats

    Formats Clone Applications Sizes available
    CD27 Antibody : RPE B30C7 F 100 Tests
    CD27 Antibody : FITC B30C7 F 0.1 mg
    CD27 Antibody : APC B30C7 F 100 Tests
    CD27 Antibody : Purified B30C7 F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)STAR117D405GA0.1 mgF, IF
      STAR117D405GA
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA928PE100 TestsF
        MCA928PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:APCMCA928APC100 TestsF
        MCA928APC
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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