TNF Alpha

TNF Alpha gallery image 1

Sandwich ELISA detection of murine TNFα using Rat anti Mouse TNFα (MCA1487) as a monoclonal capture antibody and biotinylated Rabbit anti Mouse TNFα (AAM19B) as a detection antibody with Streptavidin:HRP as the tertiary reagent for the detection of the antigen; recombinant Mouse TNFα (PMP24) at the concentrations indicated on the x-axis

  • TNF Alpha thumbnail image 1
  • Recombinant Mouse TNF Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP24E, FN, WBdatasheet pdfdatasheet pdf20 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Tumour necrosis factor alpha (TNFα) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumour cells and certain other target cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.05ng/ml20ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant mouse TNFα may be used as the standard for ELISA applications with either anti-Mouse TNFα (purified) (AAM19GA) or anti-Mouse TNFα (biotin conjugate) (AAM19B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant mouse TNFα may be used as the positive control for western blotting applications with either anti-Mouse TNFα (purified) or anti-Mouse TNFα (biotin conjugate).
    • Instructions For Use

    Additional TNF Alpha Formats

    Formats Applications Sizes available
    TNF Alpha : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Write your review

              No experiment is complete without all the pieces

              You may also be interested in...