TNF Alpha

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Sandwich ELISA detection of murine TNFα using Rat anti Mouse TNFα (MCA1487) as a monoclonal capture antibody and biotinylated Rabbit anti Mouse TNFα (AAM19B) as a detection antibody with Streptavidin:HRP as the tertiary reagent for the detection of the antigen; recombinant Mouse TNFα (PMP24) at the concentrations indicated on the x-axis

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  • Recombinant Mouse TNF Alpha
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP24E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PMP24
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Tumour necrosis factor alpha (TNFα) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumour cells and certain other target cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.05ng/ml20ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant mouse TNFα may be used as the standard for ELISA applications with either anti-Mouse TNFα (purified) (AAM19GA) or anti-Mouse TNFα (biotin conjugate) (AAM19B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant mouse TNFα may be used as the positive control for western blotting applications with either anti-Mouse TNFα (purified) or anti-Mouse TNFα (biotin conjugate).
    • Instructions For Use

    Additional TNF Alpha Formats

    Formats Applications Sizes available
    TNF Alpha : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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