Native Murine collagen I is purified Mouse collagen I from tail tendon. Thermal denaturation converts the collagen to gelatin.
Impurities: Mouse collagen type III 10% Mouse collagen (other types) <1% Non-collagenous proteins <0.5%
Purified Protein - liquid
Collagens were extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation.
SDS PAGE: 90% Cross linked collagen type I dimers and trimers represent approximately 10%.
Approx. Protein Concentrations
MW: ~300 kDa
Reagents In The Kit
Preparing The Antibody
0.5M acetic acid
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
3. Takahashi, S. et al. (2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.FEBS Lett. 589 (7): 829-35.