Murine collagen type I 45% Murine collagen type III 45% Murine collagen type IV 10% Murine collagen type V <1% Non-collagenous proteins <0.5% M[a1(I)1a2(I)2]. Native triple helix.
Purified protein from murine tail tendons - lyophilised
Use 0.05-0.5 M acetic acid, pH 2.5 at 4ºC. Dissolved collagen retains immunologic properties of native collagen types I+III. Thermal denaturation converts dissolved collagen to gelatin.
Pepsin treatment, acetic acid extraction, serial salt precipitations.
Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement and ability to form microfibrils.
Approx. Protein Concentrations
MW: 300 kDa
Reagents In The Kit
Preparing The Antibody
Essentially salt free
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.