CD62L Antibody | MEL-14

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CD62L Antibody | MEL-14 gallery image 1

Staining of mouse spleen with Rat anti Mouse CD62L: RPE (MCA1259PE)

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  • CD62L Antibody | MEL-14 thumbnail image 1
  • Rat anti Mouse CD62L
  • Rat anti Mouse CD62L:RPE
  • Rat anti Mouse CD62L:RPE
  • Rat anti Mouse CD62L
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEL-14
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1259GC, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1259G
    MCA1259PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1259PE
    MCA1259GTC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA1259GT
    MCA1259PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1259PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).

      Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays. Bio-Rad recommend a Slide-A-Lyzer® Dialysis Cassette for this purpose.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
    • Preservative Stabilisers
      Pack Size: 0.25 mg0.09% Sodium Azide (NaN3)
      Pack Size: 25 µg
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      Pack Size: 0.25 mg0.09% Sodium Azide (NaN3)
      Pack Size: 25 µg
      0.09%Sodium Azide
    • Immunogen
      Mouse B cell lymphoma, 38C-14.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Fischer rats were fused with cells of the mouse P3.X63.Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      cell adhesion
      sugar binding
      response to ATP
      external side of plasma membrane
      cell adhesion molecule binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1ug/ml
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1ug/ml
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD62L Antibody Formats

    Formats Clone Applications Sizes available
    CD62L Antibody : RPE MEL-14 F 100 Tests | 25 Tests
    CD62L Antibody : Purified MEL-14 C, F, IP 25 µg | 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
        MCA1212PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
        MCA1212PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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