CD61 Antibody | HM beta 3.1

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CD61 Antibody | HM beta 3.1 gallery image 1

Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:Alexa Fluor® ?488 (MCA2299A488)

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CD61 Antibody | HM beta 3.1 gallery image 2

Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:Alexa Fluor® 647 (MCA2299A647)

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CD61 Antibody | HM beta 3.1 gallery image 3

Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:Biotin (MCA2299B)

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CD61 Antibody | HM beta 3.1 gallery image 4

Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC (MCA2299F)

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CD61 Antibody | HM beta 3.1 gallery image 5

Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC (MCA2299FB)

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CD61 Antibody | HM beta 3.1 gallery image 6

Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:RPE (MCA2299PE)

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CD61 Antibody | HM beta 3.1 gallery image 7

Published customer image:
Phycoerythrin conjugated Hamster anti Mouse CD61 antibody, clone HM beta 3.1 (MCA2299PE) used to identify platelets in conjunction with thiazole orange for reticulated platelets by flow cytometry
Image caption:
Insulin acting at IR enhances mouse platelet function. (A) Immunoblotting confirmed ablation of IR protein expression in platelets (i) but not in liver (ii) or skeletal muscle (iii) from IRflox/flox; Pf4-Cre+ (KO) mice. KO platelet expression of IGF1R, IRS-1, 2, 3, and Akt protein were comparable to WT platelets (i, n = 3). (B) For evaluation of megakaryocyte number and morphology sections of bone marrow were imaged with a transmission electron microscope. The number of megakaryocytes per section was quantified manually using ImageJ, and expressed as the number of megakaryocytes/section corrected for total area of the section. Bars represent means ± SE (n = 3). Data were analysed by Student's unpaired t-test, and there was no significant difference in the number of megakaryocytes in WT and KO mice. Subjectively, there was no difference in the subcellular morphology of WT megakaryocytes compared with those lacking IR. Scale bars = 5 and 1 μm. (C) For evaluation of reticulated platelets by flow cytometry, whole blood from WT and KO mice was fixed, stained for CD61-PE, and incubated with thiazole orange (Retic-count™, BD Bioscience). Reticulated platelets are expressed as a percentage of thiazole orange and CD61-PE double-positive cells in 10 000 identified platelets. Bars represent mean ± SE (n = 3). Data were analysed by Student's unpaired t-test, and there was no significant difference in the number of reticulated platelets in WT and KO mice. (D and E) Monitoring of platelet aggregation using a Chronolog 590-2A aggregometer demonstrated that pre-incubation of platelets (2 × 108/mL) with insulin (20 nM, 5 min) enhances PAR-4-mediated platelet aggregation in WT (D), but not in IR KO (E) platelets (n = 3). (F and G) In agreement with platelet aggregation, PAR-4-mediated integrin αIIbβ3 activation, as measured by FACS using the JON/A antibody against the high affinity integrin αIIbβ3 conformation, was enhanced by insulin (20 nM, 5 min) in WT (F) but not in IR KO platelets (G). FACS results are expressed as mean ± SE of the percentage of maximal fluorescence of vehicle-treated platelets (n = 13). Data were analysed by two-way ANOVA (P-value), followed by Bonferroni's post-test (asterisks). A value of P < 0.05 indicates that insulin significantly altered PAR-4-mediated integrin activation in WT platelets. Asterisks (*P < 0.05, **P < 0.01, and ***P < 0.001) indicate a significant difference induced by insulin from vehicle at the matching PAR4-AP concentration. (H) Immunoprecipitation of IRS-1 demonstrates that IGF-1 (100 nM, 5 min), but not insulin (20 nM, 5 min), stimulates tyrosine phosphorylation (pY) of IRS-1 in IR KO platelets (n = 3). Membranes were reprobed to confirm equal loading.

From: Moore SF, Williams CM, Brown E, Blair TA, Harper MT, Coward RJ, Poole AW, Hers I. Loss of the insulin receptor in murine megakaryocytes/platelets causes thrombocytosis and alterations in IGF signalling.
Cardiovasc Res. 2015 Jul 1;107(1):9-19.

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  • Hamster anti Mouse CD61:FITC
  • Hamster anti Mouse CD61
  • Hamster anti Mouse CD61:FITC
  • Hamster anti Mouse CD61:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HM beta 3.1
  • Isotype
    IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2299FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2299F
    MCA2299F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2299
    MCA2299FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA2299FB
    MCA2299PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2299PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hamster anti Mouse CD61 antibody, clone HM beta 3-1 recognizes the murine integrin beta 3 subunit (CD61), a ~90 kDa a type I membrane protein, expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptor for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.

      Hamster anti Mouse CD61 antibody, clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin (Yasuda et al. 1995).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      cell-matrix adhesion
      external side of plasma membrane
      cell-substrate junction assembly
      integrin-mediated signaling pathway
      multicellular organismal development
      integrin binding
      receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/25
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD61 Antibody Formats

    Formats Clone Applications Sizes available
    CD61 Antibody : FITC HM beta 3.1 F 0.1 mg | 0.5 mg
    CD61 Antibody : Purified HM beta 3.1 F, IP 0.25 mg
    CD61 Antibody : RPE HM beta 3.1 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
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      STAR104D649GA
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      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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