CD49b Antibody | DX5

//www.bio-rad-antibodies.com/mouse-cd49b-antibody-dx5-mca2125.html?RenderPageSpecific=1
CD49b Antibody | DX5 gallery image 1

Splenocytes from a C57BL/6 SCID mouse double stained with Rat anti Mouse CD49b-FITC (MCA2125F) and MOUSE ANTI MOUSE NK1.1-R-PE (MCA1266PE)

Enlarge
CD49b Antibody | DX5 gallery image 2

Figure A. RPE conjugated rat anti mouse CD161/NK1.1 (MCA1266PE) and FITC conjugated isotype control. Figure B. RPE conjugated rat anti mouse CD161/NK1.1 (MCA1266PE) and FITC conjugated rat anti mouse CD49b (MCA2125F). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

Enlarge
CD49b Antibody | DX5 gallery image 3

Figure A. Alexa647® conjugated rat anti mouse CD94 (MCA2288A647) and FITC conjugated isotype control. Figure B. Alexa647® conjugated rat anti mouse CD94 (MCA2288A647) and FITC conjugated rat anti mouse CD49b (MCA2125F). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

Enlarge
  • CD49b Antibody | DX5 thumbnail image 1
  • CD49b Antibody | DX5 thumbnail image 2
  • CD49b Antibody | DX5 thumbnail image 3
  • Rat anti Mouse CD49b
  • Rat anti Mouse CD49b:FITC
  • Rat anti Mouse CD49b
  • Rat anti Mouse CD49b:FITC
  • Rat anti Mouse CD49b:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    DX5
  • Isotype
    IgM
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2125FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2125F
    MCA2125BFdatasheet pdfdatasheet pdf0.1 mg
    MCA2125B
    MCA2125Fdatasheet pdfdatasheet pdf0.25 mg
    MCA2125
    MCA2125TFdatasheet pdfdatasheet pdf25 µg
    MCA2125T
    MCA2125FTFdatasheet pdfdatasheet pdf25 µg
    MCA2125FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD49b monoclonal antibody, clone DX5 recognizes mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells (Pellicci et al. 2005).

      Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Arese et al. 2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoli et al. 2013).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgM - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgM from tissue culture supernatant
      Purified IgM from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      RIL-2 propagated NK1.1+ cells from C57BL/6 mice.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 0.5 mg/ml
      IgM concentration 0.1 mg/ml
      IgM concentration 0.5 mg/ml
      IgM concentration 0.1 mg/ml
      IgM concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Borate buffered saline
      Phosphate buffered saline
      Borate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Lewis rats were fused with the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC only.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC only.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integrin-mediated signaling pathway
      external side of plasma membrane
      receptor activity
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD49b Antibody Formats

    Formats Clone Applications Sizes available
    CD49b Antibody : Purified DX5 F 0.25 mg | 25 µg
    CD49b Antibody : Biotin DX5 F 0.1 mg
    CD49b Antibody : FITC DX5 F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116A0.1 mgE, WB
      STAR116A
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116A0.1 mgE, WB
      STAR116A
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Write your review

              You may also be interested in...