CD31 antibody | CO.3E1D4
Mouse anti Sheep CD31:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- CO.3E1D4
- Isotype
- IgG2a
- Specificity
- CD31
Antibody Quality and Performance Guarantee
Fitc conjugated Mouse anti Sheep CD31 antibody, clone CO.3E1D4 (MCA1097F) used to evaluate CD31 expression on ovine adipose tissue derived stem cells by flow cytometry.
Image caption:
Cytofluorimetric characterization of oADSCs at P1 and P6 of culture in GM. (A) Graphical representations of the flow cytometry analyses conducted on oADSCs at P1 regarding stemness, mesenchymal (adhesion molecules), hematopoietic, and MHC markers. Histograms in blue represent stained cells, while the red ones represent the control of the un-stained group. (B) Histogram representation of the analyzed markers within oADSCs (P1 and P6) expressed as the mean fluorescence intensity (MFI) ratio. The red line represents the positivity threshold. Data were obtained as means ± SD values of 3 replicates for each sample.
From:Haidar-Montes AA, Mauro A, El Khatib M, Prencipe G, Pierdomenico L, Tosi U, Wouters G, Cerveró-Varona A, Berardinelli P, Russo V, Barboni B.
Mechanobiological Strategies to Enhance Ovine (Ovis aries) Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications.
Animals (Basel). 2024 Jul 31;14(15):2233.
doi: 10.3390/ani14152233.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F Reset| Mouse anti Sheep CD31 antibody, clone CO.3E1D4 recognizes ovine CD31, also known as PECAM-1. Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells. Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (Pintado et al. 1995). |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Ovine leucocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Source Reference
-
Pintado. C. O. et al. (1995) A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes.
Immunol. Lett. 45: 81 - 85.
References for CD31 antibody
-
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species:
Vet. Immunol. Immunopathol. 64: 1-13. -
Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
Immunology. 113: 310-7. -
Summers, C. et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 106 (3-4): 285-94. -
Zannettino, A.C. et al. (2010) Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.
Tissue Eng Part A. 16 (12): 3579-87. -
De Visscher, G. et al. (2010) Selection of an immunohistochemical panel for cardiovascular research in sheep.
Appl Immunohistochem Mol Morphol. 18: 382-91. -
Filby, C.E. et al. (2010) Partial pulmonary embolization disrupts alveolarization in fetal sheep.
Respir Res. 11: 42. -
Boos, A.M. et al. (2011) Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model.
J Cell Mol Med. 15 (6): 1364-78. -
Berardinelli, P. et al. (2013) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation.
Clin Oral Investig. 17 (7): 1661-75.
View The Latest Product References
-
Barboni, B. et al. (2013) Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation.
PLoS One. 8 (5): e63256. -
Lasecka L et al. (2015) Antibodies to the core proteins of nairobi sheep disease virus/ganjam virus reveal details of the distribution of the proteins in infected cells and tissues.
PLoS One. 10 (4): e0124966. -
Iablonskii, P. et al. (2015) Tissue-engineered mitral valve: morphology and biomechanics †.
Interact Cardiovasc Thorac Surg. 20 (6): 712-9; discussion 719. -
Weigand, A. et al. (2017) Bone Tissue Engineering Under Xenogeneic-Free Conditions in a Large Animal Model as a Basis for Early Clinical Applicability.
Tissue Eng Part A. 23 (5-6): 208-22. -
Nielsen, E.Ø. et al. (2018) Optimizing Osteogenic Differentiation of Ovine Adipose-Derived Stem Cells by Osteogenic Induction Medium and FGFb, BMP2, or NELL1 In Vitro.
Stem Cells Int. 2018: 9781393. -
López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
Tissue Eng Part A. 26 (17-18): 993-1004. -
Zhang, T.M. et al. (2024) YAP promotes the early development of temporomandibular joint bony ankylosis by regulating mesenchymal stem cell function.
Sci Rep. 14 (1): 12704. -
Haidar-Montes, A.A. et al. (2024) Mechanobiological Strategies to Enhance Ovine (Ovis aries) Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications.
Animals (Basel). 14 (15)Jul 31 [Epub ahead of print]. -
Park, S. et al. (2026) Machine learning framework for passage-based quality control of Hanwoo muscle satellite cells for cultured meat.
Food Res Int. 231 (Pt 2): 118616.
- Synonyms
- PECAM-1
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