CD25 antibody | 9.14

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Mouse anti Sheep CD25:FITC

Mouse anti Sheep CD25

Product Type
Monoclonal Antibody
Clone
9.14
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2218F F 0.1 mg
MCA2218GA C F IP 0.1 mg
Mouse anti sheep CD25, clone 9.14, is specific for the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Rα), a single pass type I membrane protein expressed by activated ovine T-cells.

Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132). A non-covalent association of the α and β subunits is required to form the high affinity receptor for IL-2.

Immunoprecipitation studies have demonstrated that clone 9.14 precipitates a band of approximately 47 kDa under reducing conditions, which is that expected of the mature protein due to high glycosylation, and is consistent with the observed molecular weight of IL-2Rα in other species such as humans (Verhagen et. al.1993).

Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011).

Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25 and may be of used in facilitating further studies of T-regs in this species.

Product Details

Target Species
Sheep
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch

More Information

UniProt
P26898 Related reagents
Entrez Gene
IL2RA Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
Regulatory
For research purposes only

Applications of CD25 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD25 antibody

  1. Verhagen, A.M. et al. (1993) Characterization of the ovine interleukin-2 receptor-alpha chain: differential induction on precultured alpha beta and gamma delta T cells.
    Immunology. 79 (3): 471-8.
  2. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
    Clin Vaccine Immunol. 17: 108-17.
  3. Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation
    Immunology 113: 310-7.
  4. Gillan, S. et al. (2010) Ovine immune parameters following immunisation against Mycobacterium avium ssp. paratuberculosis using a lipid-based live-cell vaccine.
    Vet Immunol Immunopathol. 137 (1-2): 109-19.

Further Reading

  1. Rocchi, M.S. et al. (2011) Identification of CD4+CD25 high Foxp3+ T cells in ovine peripheral blood.
    Vet Immunol Immunopathol. 144 (1-2): 172-7.