Sarilumab antibody | AbD49514ia
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Sarilumab Antibody, clone AbD49514ia (HCA410) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002).
A microtiter plate was coated overnight with sarilumab, tocilizumab, infliximab, human IgG1 kappa control and human IgG1 lambda control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Sarilumab Antibody, clone AbD49514ia (HCA410) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for sarilumab are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002).
A microtiter plate was coated over night with sarilumab at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Sarilumab Antibody, clone AbD49514ia (HCA410, black), clone AbD49516ia (HCA411, blue), and clone AbD49520ia (HCA412, red). Detection was performed using HRP conjugated sarilumab at a concentration of 2 µg/mL in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002). Data is shown as the mean of three measurements.
A microtiter plate was coated over night with recombinant human IL-6 receptor at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of sarilumab (0.144 µg/mL) plus increasing concentrations of Human Anti-Sarilumab Antibody, clone AbD49514ia (HCA410) in the monovalent Fab format was added. Free sarilumab, still capable of binding to the human IL-6 receptor coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) and QuantaBlu fluorogenic peroxidase substrate. Data is shown as the mean of three measurements.
Human anti Sarilumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD49514ia
- Isotype
- IgG1
- Specificity
- Sarilumab
Antibody Quality and Performance Guarantee
HCA410
| Human Anti-Sarilumab Antibody, clone AbD49514ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug sarilumab, and its biosimilar products. It does not recognize the drug target, the human Interleukin 6 receptor (IL-6R), nor sarilumab in complex with IL-6R. This antibody can be used to measure free sarilumab levels in serum from patients. HCA410 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of <0.1nM and is one of the three antibodies offered for ADA assays. Antibody HCA411 has an affinity of 0.9nM and HCA412 has an affinity of 2nM. Sarilumab (trade name Kevzara) is a humanized monoclonal antibody of the IgG1/kappa isotype that suppresses the inflammatory effects of interleukin-6 (IL-6) by binding to both membrane bound and soluble IL-6 receptor forms, thus blocking cis- and trans-inflammatory signaling cascades. Through this, sarilumab blocks the IL-6 signaling pathway (measured as STAT-3 inhibition). It is indicated for the treatment of moderate to severe reactive Rheumatoid Arthritis (RA) in adult patients who are irresponsive, respond inadequately or present intolerance to disease-modifying anti-rheumatic drugs (DMARDs) or tumor necrosis factor (TNF) antagonists. It is also indicated in combination with methotrexate (MTX) or as a monotherapy when there is intolerance to MTX or when MTX administration is inappropriate. |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This product is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Sarilumab
- Affinity
- The monovalent intrinsic affinity of Human Anti-Sarilumab Antibody, clone AbD49514ia was measured as KD = <0.1 nM by real time, label-free molecular interaction analysis on immobilized sarilumab
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Kevzara is a registered trademark of Sanofi and Regeneron Pharmaceuticals - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Clone AbD49514ia can be used in indirect ELISA to detect sarilumab. It can also be used as a surrogate positive control or a calibrator in an anti-drug antibody (ADA) assay, together with HCA411 and HCA412
- Synonyms
- Kevzara
HCA410
168426
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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