Romosozumab antibody | AbD59921
Human anti Romosozumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD59921
- Isotype
- Fab antibody
- Specificity
- Romosozumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Romosozumab Antibody, clone AbD59921pap (TZA099P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with romosozumab, Sclerostin-His, and human IgG2 kappa control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, the Sclerostin-His/romosozumab complex was formed by adding 5 µg/mL romosozumab to the wells coated with 5 µg/mL Sclerostin-His. Detection was performed using Human Anti-Romosozumab (Drug/Target Complex) Antibody, clone AbD59921ad (TZA099) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for Sclerostin-His/romosozumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Sclerostin-His at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of romosozumab. Detection was performed using HRP-conjugated Human Anti-Romosozumab (Drug/Target Complex) Antibody, clone AbD59921pap (TZA099P) at a concentration of 0.2 µg/mL in HISPEC Assay Diluent (BUF049A), and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Romosozumab (Drug/Target Complex) Antibody, clone AbD59921 specifically recognizes the antibody drug romosozumab when in complex with its target, . It does not recognize free romosozumab nor unbound sclerostin. The monovalent Fab format of this antibody, clone AbD59921ad (TZA099) is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. This antibody is also available as a bivalent Fab antibody pre-coupled to the HRP-conjugated BiSpyCatcher2 (TZC002P) under product code TZA099P for direct detection of the Romosozumab/Sclerostin complex in bioanalysis assays. Clone AbD59921 can be used in pharmacokinetic (PK) antigen capture format assays to measure romosozumab that has been captured via immobilized sclerostin. It is ideal for bioanalytical assays to measure the level of drug or biosimilar in complex with the sclerostin target. Romosozumab (trade name Evenity) is a humanized monoclonal antibody of the IgG2/kappa isotype that binds to sclerostin and prevents inhibition of the Wnt signaling pathway, thereby promoting osteogenesis and, to a lesser extent, reducing bone resorption. Romosozumab's inhibition of sclerostin also inhibits the increase in RANKL-dependent increases in osteoclast activity and bone resorption. Romosozumab is indicated for the treatment of osteoporosis in postmenopausal women at high risk for fracture. View a summary of all anti-romosozumab antibodies |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Romosozumab/Sclerostin complex
- Affinity
- The monovalent intrinsic affinity of AbD59921ad was measured as KD = 12 nM by real time, label free molecular interaction analysis on immobilized human Sclerostin/romosozumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Evenity is a trademark of Amgen and UCB. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD59921ad may be used in an indirect ELISA to detect the human Sclerostin/romosozumab complex or to detect romosozumab bound to immobilized human Sclerostin.
Protocol: PK antigen capture ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| Human anti Romosozumab | HCA423 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Romosozumab | ||||||
| Human anti Romosozumab | TZA060 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Romosozumab | ||||||
| Human anti Romosozumab | TZA061 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Romosozumab | ||||||
| Human anti Romosozumab:HRP | TZA061P | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Romosozumab:HRP | ||||||
| Human anti Romosozumab (Drug/Target Complex):HRP | TZA099P | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Romosozumab (Drug/Target Complex):HRP | ||||||
- Synonyms
- Evenity
TZA099
171359
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Romosozumab specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up
