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Granulocytes antibody | HIS48

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Anti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48 thumbnail image 1 Anti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48 thumbnail image 2
Anti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48 gallery image 1
Published customer image:
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 (MCA967) used for staining granulocytes in rat corneal sections by immunofluorescence.
Image caption:
Immunofluorescence images on flat-mounted corneas in a model of endotoxin-induced uveitis analyzed with a 3D laser confocal microscope with a 20X enlargement. Immunostainings with anti-CD68 for macrophages (A), anti-TCR alpha/beta for lymphocytes (B), anti-MCA967 for granulocytes (C), and IgG1 mouse antibody for isotypic control (D) were revealed with secondary antibody, Alexa Fluor (in green) while the nuclear chromatin was stained with propidium iodide (in red). A double immunostaining with Alexa Fluor 488 phalloidin (in green), and antibody to CD68 (in red) was performed to localize the macrophages among the corneal layers, notably on the endothelium (E).

From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 2008 Jun16;14:1149-56.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48 gallery image 2
Published customer image:
Mouse anti Rat CD68 antibody, clone ED1 (MCA967R) used to label macrophages and monocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Subcapsular sinus of the rat popliteal lymph node stained for the presence of granulocytes (HiS48+, red). (A) The node of the control animal receiving 0.9% NaCl (days 1–7, node evaluation on day 8). (B) The node after initial massive S. epidermidis infection (days 1–7) with early node evaluation (day 8). (C) The node after secondary S. epidermidis infection (day 28, node evaluation on day 29). Magnification 200×.

From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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IF C

Mouse anti Rat Granulocytes and Erythroid Cells

Product Type
Monoclonal Antibody
Clone
HIS48
Isotype
IgM
Specificity
Granulocytes

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA967
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF P * 2 ml loader
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Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 recognizes granulocytes and erythroid cells.

Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).

Target Species
Rat
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
PVG rat spleen cell suspension.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry neat
Immunofluorescence
Immunohistology - Frozen 1 1/20
Immunohistology - Paraffin 2
  1. 1The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends PLP fixation for paraffin sections. See Whiteland et al., 1995 and Banerjee et al., 2003 for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to stain 106 cells in 100ul.

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References for Granulocytes antibody

  1. van Goor, H. et al. (1991) Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids.
    Lab Invest. 64 (6): 754-65.
  2. Reckless, J. et al. (2001) The pan-chemokine inhibitor NR58-3.14.3 abolishes tumour necrosis factor-alpha accumulation and leucocyte recruitment induced by lipopolysaccharide in vivo.
    Immunology. 103 (2): 244-54.
  3. Dimitrijević, M. et al. (2010) Modulation of granulocyte functions by peptide YY in the rat: age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity.
    Regul Pept. 159: 100-9.
  4. Howard, K.M. et al. (2009) Differential expression of platelet-activating factor acetylhydrolase in lung macrophages.
    Am J Physiol Lung Cell Mol Physiol. 297: L1141-50.
  5. Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
    Mol Vis. 14: 1149-56.
  6. Narita, T. et al. (2012) The use of cell-sheet technique eliminates arrhythmogenicity of skeletal myoblast-based therapy to the heart with enhanced therapeutic effects.
    Int J Cardiol. pii: S0167-5273(12)01187-4.
  7. Foucher, P. et al. (1999) Antimyeloperoxidase-associated Lung Disease
    An Experimental Model
    Am J Respir Crit Care Med. 160: 987-94.
  8. Della Coletta Francescato, H. et al. (2011) Inhibition of hydrogen sulphide formation reduces cisplatin-induced renal damage.
    Nephrol Dial Transplant. 26: 479-88.
    9. View The Latest Product References
  9. Gering, K.M. et al. (2006) The interaction mode of premalignant Schwann and immune effector cells during chemically induced carcinogenesis in the rat peripheral nervous system is strongly influenced by genetic background.
    Cancer Res. 66: 4708-14.
  10. Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
    Diabetes. 55: 1625-33.
  11. Panichi, V. et al. (2001) Effects of 1,25(OH)2D3 in experimental mesangial proliferative nephritis in rats.
    Kidney Int. 60: 87-95.
  12. van der Kaaij, N.P. et al. (2005) Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung.
    Eur J Cardiothorac Surg. 27: 774-82.
  13. Pauly, A. et al. (2007) New tools for the evaluation of toxic ocular surface changes in the rat.
    Invest Ophthalmol Vis Sci. 48: 5473-83.
  14. Nakagawa, K. et al. (2002) Lecithinized superoxide dismutase reduces cold ischemia-induced chronic allograft dysfunction.
    Kidney Int. 61: 1160-9.
  15. Dugast, A.S. et al. (2008) Myeloid-derived suppressor cells accumulate in kidney allograft tolerance and specifically suppress effector T cell expansion.
    J Immunol. 180: 7898-906.
  16. Ysebaert, D.K. et al. (2000) Identification and kinetics of leukocytes after severe ischaemia/reperfusion renal injury.
    Nephrol Dial Transplant. 15: 1562-74.
  17. Szczesny, G. et al. (2004) Limb lymph node response to bone fracture.
    Lymphat Res Biol. 2: 155-64.
  18. Steen, P.W. et al. (2010) Neutrophil responses to injury or inflammation impair peripheral gustatory function.
    Neuroscience. 167: 894-908.
  19. Cantaluppi V et al. (2015) Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis.
    Nephrol Dial Transplant. 30 (3): 410-22.
  20. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.

Further Reading

  1. Kampinga, J. et al. (1990) Thymocyte differentiation and thymic micro-environment development in the foetal rat thymus: an immunohistological approach. thymus in tolerance induction.
    In: The role of the Thymus Update 3. Eds. M.D. Kendall and M.A. Ritter. Harwood Academic Publishers GmbH, Switzerland.

Immunofluorescence

Immunohistology - Frozen

RRID
AB_322077
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