Granulocytes antibody | HIS48
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 (MCA967) used for staining granulocytes in rat corneal sections by immunofluorescence.
Image caption:
Immunofluorescence images on flat-mounted corneas in a model of endotoxin-induced uveitis analyzed with a 3D laser confocal microscope with a 20X enlargement. Immunostainings with anti-CD68 for macrophages (A), anti-TCR alpha/beta for lymphocytes (B), anti-MCA967 for granulocytes (C), and IgG1 mouse antibody for isotypic control (D) were revealed with secondary antibody, Alexa Fluor (in green) while the nuclear chromatin was stained with propidium iodide (in red). A double immunostaining with Alexa Fluor 488 phalloidin (in green), and antibody to CD68 (in red) was performed to localize the macrophages among the corneal layers, notably on the endothelium (E).
From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 2008 Jun16;14:1149-56.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 recognizes granulocytes and erythroid cells.
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).
Product Details
- Target Species
- Rat
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- PVG rat spleen cell suspension.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Granulocytes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | neat | ||
| Immunofluorescence | |||
| Immunohistology - Frozen 1 | 1/20 | ||
| Immunohistology - Paraffin 2 |
- 1The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends PLP fixation for paraffin sections. See Whiteland et al., 1995 and Banerjee et al., 2003 for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to stain 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | ||
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C E P WB | ||
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | ||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C E WB | ||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E |
Application Based External Images
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for Granulocytes antibody
-
van Goor, H. et al. (1991) Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids.
Lab Invest. 64 (6): 754-65. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87: 1515-22. -
Reckless, J. et al. (2001) The pan-chemokine inhibitor NR58-3.14.3 abolishes tumour necrosis factor-alpha accumulation and leucocyte recruitment induced by lipopolysaccharide in vivo.
Immunology. 103 (2): 244-54. -
Dimitrijević, M. et al. (2010) Modulation of granulocyte functions by peptide YY in the rat: age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity.
Regul Pept. 159: 100-9. -
Howard, K.M. et al. (2009) Differential expression of platelet-activating factor acetylhydrolase in lung macrophages.
Am J Physiol Lung Cell Mol Physiol. 297: L1141-50. -
Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 14: 1149-56. -
Narita, T. et al. (2012) The use of cell-sheet technique eliminates arrhythmogenicity of skeletal myoblast-based therapy to the heart with enhanced therapeutic effects.
Int J Cardiol. pii: S0167-5273(12)01187-4. -
Foucher, P. et al. (1999) Antimyeloperoxidase-associated Lung Disease
An Experimental Model
Am J Respir Crit Care Med. 160: 987-94. -
Della Coletta Francescato, H. et al. (2011) Inhibition of hydrogen sulphide formation reduces cisplatin-induced renal damage.
Nephrol Dial Transplant. 26: 479-88. -
Gering, K.M. et al. (2006) The interaction mode of premalignant Schwann and immune effector cells during chemically induced carcinogenesis in the rat peripheral nervous system is strongly influenced by genetic background.
Cancer Res. 66: 4708-14. -
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 55: 1625-33. -
Panichi, V. et al. (2001) Effects of 1,25(OH)2D3 in experimental mesangial proliferative nephritis in rats.
Kidney Int. 60: 87-95. -
van der Kaaij, N.P. et al. (2005) Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung.
Eur J Cardiothorac Surg. 27: 774-82. -
Pauly, A. et al. (2007) New tools for the evaluation of toxic ocular surface changes in the rat.
Invest Ophthalmol Vis Sci. 48: 5473-83. -
Nakagawa, K. et al. (2002) Lecithinized superoxide dismutase reduces cold ischemia-induced chronic allograft dysfunction.
Kidney Int. 61: 1160-9. -
Dugast, A.S. et al. (2008) Myeloid-derived suppressor cells accumulate in kidney allograft tolerance and specifically suppress effector T cell expansion.
J Immunol. 180: 7898-906. -
Ysebaert, D.K. et al. (2000) Identification and kinetics of leukocytes after severe ischaemia/reperfusion renal injury.
Nephrol Dial Transplant. 15: 1562-74. -
Szczesny, G. et al. (2004) Limb lymph node response to bone fracture.
Lymphat Res Biol. 2: 155-64. -
Steen, P.W. et al. (2010) Neutrophil responses to injury or inflammation impair peripheral gustatory function.
Neuroscience. 167: 894-908. -
Cantaluppi V et al. (2015) Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis.
Nephrol Dial Transplant. 30 (3): 410-22.
Further Reading
-
Kampinga, J. et al. (1990) Thymocyte differentiation and thymic micro-environment development in the foetal rat thymus: an immunohistological approach. thymus in tolerance induction.
In: The role of the Thymus Update 3. Eds. M.D. Kendall and M.A. Ritter. Harwood Academic Publishers GmbH, Switzerland.
