Granulocytes antibody | HIS48

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Mouse anti Rat Granulocytes and Erythroid Cells

Product Type
Monoclonal Antibody
Clone
HIS48
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA967 C* F IF P* 2 ml
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 recognizses granulocytes and erythroid cells.

Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).

Product Details

Target Species
Rat
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
PVG rat spleen cell suspension.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Granulocytes antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the
antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry neat
Immunofluorescence
Immunohistology - Frozen 1 1/20
Immunohistology - Paraffin 2
  1. 1The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends PLP fixation for paraffin sections. See Whiteland et al., 1995 and Banerjee et al., 2003 for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to stain 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for Granulocytes antibody

  1. van Goor, H. et al. (1991) Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids.
    Lab Invest. 64 (6): 754-65.
  2. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 87: 1515-22.
  3. Reckless, J. et al. (2001) The pan-chemokine inhibitor NR58-3.14.3 abolishes tumour necrosis factor-alpha accumulation and leucocyte recruitment induced by lipopolysaccharide in vivo.
    Immunology. 103 (2): 244-54.
  4. Dimitrijević, M. et al. (2010) Modulation of granulocyte functions by peptide YY in the rat: age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity.
    Regul Pept. 159: 100-9.
  5. Howard, K.M. et al. (2009) Differential expression of platelet-activating factor acetylhydrolase in lung macrophages.
    Am J Physiol Lung Cell Mol Physiol. 297: L1141-50.
  6. Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
    Mol Vis. 14: 1149-56.
  7. Narita, T. et al. (2012) The use of cell-sheet technique eliminates arrhythmogenicity of skeletal myoblast-based therapy to the heart with enhanced therapeutic effects.
    Int J Cardiol. pii: S0167-5273(12)01187-4.
  8. Foucher, P. et al. (1999) Antimyeloperoxidase-associated Lung Disease
    An Experimental Model
    Am J Respir Crit Care Med. 160: 987-94.
  9. Della Coletta Francescato, H. et al. (2011) Inhibition of hydrogen sulphide formation reduces cisplatin-induced renal damage.
    Nephrol Dial Transplant. 26: 479-88.
  10. Gering, K.M. et al. (2006) The interaction mode of premalignant Schwann and immune effector cells during chemically induced carcinogenesis in the rat peripheral nervous system is strongly influenced by genetic background.
    Cancer Res. 66: 4708-14.
  11. Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
    Diabetes. 55: 1625-33.
  12. Panichi, V. et al. (2001) Effects of 1,25(OH)2D3 in experimental mesangial proliferative nephritis in rats.
    Kidney Int. 60: 87-95.
  13. van der Kaaij, N.P. et al. (2005) Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung.
    Eur J Cardiothorac Surg. 27: 774-82.
  14. Pauly, A. et al. (2007) New tools for the evaluation of toxic ocular surface changes in the rat.
    Invest Ophthalmol Vis Sci. 48: 5473-83.
  15. Nakagawa, K. et al. (2002) Lecithinized superoxide dismutase reduces cold ischemia-induced chronic allograft dysfunction.
    Kidney Int. 61: 1160-9.
  16. Dugast, A.S. et al. (2008) Myeloid-derived suppressor cells accumulate in kidney allograft tolerance and specifically suppress effector T cell expansion.
    J Immunol. 180: 7898-906.
  17. Ysebaert, D.K. et al. (2000) Identification and kinetics of leukocytes after severe ischaemia/reperfusion renal injury.
    Nephrol Dial Transplant. 15: 1562-74.
  18. Szczesny, G. et al. (2004) Limb lymph node response to bone fracture.
    Lymphat Res Biol. 2: 155-64.
  19. Steen, P.W. et al. (2010) Neutrophil responses to injury or inflammation impair peripheral gustatory function.
    Neuroscience. 167: 894-908.
  20. Cantaluppi V et al. (2015) Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis.
    Nephrol Dial Transplant. 30 (3): 410-22.

Further Reading

  1. Kampinga, J. et al. (1990) Thymocyte differentiation and thymic micro-environment development in the foetal rat thymus: an immunohistological approach. thymus in tolerance induction.
    In: The role of the Thymus Update 3. Eds. M.D. Kendall and M.A. Ritter. Harwood Academic Publishers GmbH, Switzerland.