Granulocytes antibody | HIS48
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).
Product Details
- Target Species
- Rat
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- PVG rat spleen cell suspension.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Granulocytes antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | neat | ||
Immunofluorescence | |||
Immunohistology - Frozen 1 | 1/20 | ||
Immunohistology - Paraffin 2 |
- 1The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2The epitope recognised by this antibody is reported to be sensitive to routine formaldehyde-based fixation and tissue processing. Bio-Rad recommends PLP fixation for paraffin sections. See Whiteland et al., 1995 and Banerjee et al., 2003 for details.
- Flow Cytometry
- Use 10ul of the suggested working dilution to stain 106 cells in 100ul.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
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Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
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Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
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Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
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Product Specific References
References for Granulocytes antibody
-
van Goor, H. et al. (1991) Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids.
Lab Invest. 64 (6): 754-65. -
Reckless, J. et al. (2001) The pan-chemokine inhibitor NR58-3.14.3 abolishes tumour necrosis factor-alpha accumulation and leucocyte recruitment induced by lipopolysaccharide in vivo.
Immunology. 103 (2): 244-54. -
Dimitrijević, M. et al. (2010) Modulation of granulocyte functions by peptide YY in the rat: age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity.
Regul Pept. 159: 100-9. -
Howard, K.M. et al. (2009) Differential expression of platelet-activating factor acetylhydrolase in lung macrophages.
Am J Physiol Lung Cell Mol Physiol. 297: L1141-50. -
Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 14: 1149-56. -
Narita, T. et al. (2012) The use of cell-sheet technique eliminates arrhythmogenicity of skeletal myoblast-based therapy to the heart with enhanced therapeutic effects.
Int J Cardiol. pii: S0167-5273(12)01187-4. -
Foucher, P. et al. (1999) Antimyeloperoxidase-associated Lung Disease
An Experimental Model
Am J Respir Crit Care Med. 160: 987-94. -
Della Coletta Francescato, H. et al. (2011) Inhibition of hydrogen sulphide formation reduces cisplatin-induced renal damage.
Nephrol Dial Transplant. 26: 479-88. -
Gering, K.M. et al. (2006) The interaction mode of premalignant Schwann and immune effector cells during chemically induced carcinogenesis in the rat peripheral nervous system is strongly influenced by genetic background.
Cancer Res. 66: 4708-14. -
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 55: 1625-33. -
Panichi, V. et al. (2001) Effects of 1,25(OH)2D3 in experimental mesangial proliferative nephritis in rats.
Kidney Int. 60: 87-95. -
van der Kaaij, N.P. et al. (2005) Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung.
Eur J Cardiothorac Surg. 27: 774-82. -
Pauly, A. et al. (2007) New tools for the evaluation of toxic ocular surface changes in the rat.
Invest Ophthalmol Vis Sci. 48: 5473-83. -
Nakagawa, K. et al. (2002) Lecithinized superoxide dismutase reduces cold ischemia-induced chronic allograft dysfunction.
Kidney Int. 61: 1160-9. -
Dugast, A.S. et al. (2008) Myeloid-derived suppressor cells accumulate in kidney allograft tolerance and specifically suppress effector T cell expansion.
J Immunol. 180: 7898-906. -
Ysebaert, D.K. et al. (2000) Identification and kinetics of leukocytes after severe ischaemia/reperfusion renal injury.
Nephrol Dial Transplant. 15: 1562-74. -
Szczesny, G. et al. (2004) Limb lymph node response to bone fracture.
Lymphat Res Biol. 2: 155-64. -
Steen, P.W. et al. (2010) Neutrophil responses to injury or inflammation impair peripheral gustatory function.
Neuroscience. 167: 894-908. -
Cantaluppi V et al. (2015) Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis.
Nephrol Dial Transplant. 30 (3): 410-22.
Further Reading
-
Kampinga, J. et al. (1990) Thymocyte differentiation and thymic micro-environment development in the foetal rat thymus: an immunohistological approach. thymus in tolerance induction.
In: The role of the Thymus Update 3. Eds. M.D. Kendall and M.A. Ritter. Harwood Academic Publishers GmbH, Switzerland.
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