CD90 antibody | T11D7e

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Mouse anti Rat CD90

Product Type
Monoclonal Antibody
Clone
T11D7e
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA04G CT F 0.25 mg
MCA04GT CT F 25 µg
Mouse anti Rat CD90 antibody, clone T11D7e recognizes mouse and rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Clone T11D7e reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c.

Clone T11D7e is useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake et al. 1979).

Mouse anti Rat CD90 antibody, clone T11D7e is routinely tested in flow cytometry using mouse thymocytes.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Xenogeneic rat thymocytes.
Approx. Protein Concentrations
IgM concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised CBA mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01830 Related reagents
Entrez Gene
Thy1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005829 cytosol
GO:0005100 Rho GTPase activator activity
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0019901 protein kinase binding
GO:0030336 negative regulation of cell migration
GO:0030426 growth cone
GO:0034235 GPI anchor binding
GO:0043066 negative regulation of apoptosis
GO:0043547 positive regulation of GTPase activity
GO:0045576 mast cell activation
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0048147 negative regulation of fibroblast proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
Regulatory
For research purposes only

Applications of CD90 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Cytotoxic Assays
Flow Cytometry 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Product Specific References

References for CD90 antibody

  1. Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.
    Eur J Immunol. 9 (11): 875-86.
  2. Mahanthappa, N.K. et al. (1996) Glial growth factor 2, a soluble neuregulin, directly increases Schwann cell motility and indirectly promotes neurite outgrowth.
    J Neurosci. 16: 4673-83.
  3. Yasumizu, R. et al. (1988) Development of donor-derived thymic lymphomas after allogeneic bone marrow transplantation in AKR/J mice.
    J Immunol. 141 (6): 2181-6.
  4. Fabian, R.H. (1990) Uptake of antineuronal IgM by CNS neurons: comparison with antineuronal IgG.
    Neurology. 40 (3 Pt 1): 419-22.
  5. Ogasawara, K. et al. (1990) Ia restriction specificity of KLH-specific T cells from allogeneic bone marrow chimeras is influenced by histocompatibility at the H-2 and minor histocompatibility loci.
    Microbiol Immunol. 34 (12): 1025-39.
  6. Heinen, A. et al. (2015) Fingolimod induces the transition to a nerve regeneration promoting Schwann cell phenotype.
    Exp Neurol. 271: 25-35.
  7. Dietrich, N. et al. (2016) The DPP4 Inhibitor Linagliptin Protects from Experimental Diabetic Retinopathy.
    PLoS One. 11 (12): e0167853.
  8. Pasten, C. et al. (2015) ApoER2 and Reelin are expressed in regenerating peripheral nerve and regulate Schwann cell migration by activating the Rac1 GEF protein, Tiam1.
    Mol Cell Neurosci. 69: 1-11.
  9. Deliyanti, D. & Wilkinson-Berka, J.L. (2015) Inhibition of NOX1/4 with GKT137831: a potential novel treatment to attenuate neuroglial cell inflammation in the retina.
    J Neuroinflammation. 12: 136.
  10. Simitzi, C. et al. (2015) Data in support on the shape of Schwann cells and sympathetic neurons onto microconically structured silicon surfaces.
    Data Brief. 4: 636-40.
  11. Dietrich, N. et al. (2016) The DPP4 Inhibitor Linagliptin Protects from Experimental Diabetic Retinopathy.
    PLoS One. 11 (12): e0167853.