Mouse anti rabbit MHC class II monoclonal antibody, clone 2C4 recognizes the class II RLA-DQ molecule. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rabbits, this complex is referred to as the rabbit leukocyte antigen (RLA) region. There are 3 major MHC class II proteins encoded by the RLA which are RLA DP, RLA DQ and RLA DR.
Mouse anti rabbit MHC class II recognizes the RLA DQ molecule, expressed by antigen presenting cells, B cells and monocytes.
This antibody is reported to inhibit the mixed lymphocyte reaction by 80%. Immunoprecipitation studies yield three bands of molecular weight 28, 31 and 35 kDa.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Rabbit spleen cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63. Ag8.653 mouse myeloma cell line
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MHC Class II DQ antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for MHC Class II DQ antibody
Lobel, S.A. et al. (1984) The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody.
Immunology 51: 35-43.
Spieker-Polet, H. et al. (1990) Rabbit major histocompatibility complex. IV. Expression of major histocompatibility complex class II genes.
J Immunogenet. 17 (1-2): 123-32.
Matsumura, T. et al. (1999) Suppression of atherosclerotic development in Watanabe heritable hyperlipidemic rabbits treated with an oral antiallergic drug, tranilast.
Circulation 99 (7): 919-24.
Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
Biotechnol Prog. 26 (6): 1749-58.
Andersen, H.O. et al. (1999) Effect of cyclosporine on arterial balloon injury lesions in cholesterol-clamped rabbits: T lymphocyte-mediated immune responses not involved in balloon injury-induced neointimal proliferation.
Arterioscler Thromb Vasc Biol. 19: 1687-94.
Alt, C. et al. (2009) Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.
Curr HIV Res. 7: 639-49.
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology 77: 106-8.
Idogawa, H. et al. (1997) Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits.
Scand J Immunol. 46: 572-80.
Shen, W. et al. (2013) Osteoarthritis prevention through meniscal regeneration induced by intra-articular injection of meniscus stem cells.
Stem Cells Dev. 22 (14): 2071-82.