MHC Class II DQ antibody | 2C4
Mouse anti Rabbit MHC class II antibody, clone 2C4 (MCA811GA) used to assess MHC Class-II expression in native and vacuum‐assisted decellularized trachea (VADT) tracheal grafts in rabbits by immunofluorescence.
Image caption:
Immunological evaluation of native and VADT grafts. (b) Immunofluorescence staining of DAPI/Col‐II (G, H), DAPI/MHC‐I (I, J), and DAPI/MHC‐II (K, L) expressed in the native trachea and VADT scaffolds.
From: Sun F, Shen Z, Zhang B, Lu Y, Shan Y, Wu Q, Yuan L, Zhu J, Pan S, Wang Z, Wu C, Zhang G, Yang W, Xu X, Shi H.
Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step.
Bioeng Transl Med. 2023 May 3;8(4):e10534.
doi: 10.1002/btm2.10534..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit MHC Class II DQ
- Product Type
- Monoclonal Antibody
- Clone
- 2C4
- Isotype
- IgG2a
- Specificity
- MHC Class II DQ
Antibody Quality and Performance Guarantee
| Mouse anti Rabbit MHC class II monoclonal antibody, clone 2C4 recognizes the class II RLA-DQ molecule. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rabbits, this complex is referred to as the rabbit leukocyte antigen (RLA) region. There are 3 major MHC class II proteins encoded by the RLA which are RLA DP, RLA DQ and RLA DR. Mouse anti rabbit MHC class II recognizes the RLA DQ molecule, expressed by antigen presenting cells, B cells and monocytes. Mouse anti rabbit MHC class II monoclonal antibody, clone 2C4 is able to significantly inhibit the mixed lymphocyte reaction. Immunoprecipitation with the antibody yields three bands of molecular weights 28, 31 and 35 kDa. |
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rabbit spleen cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63. Ag8.653 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
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Source Reference
-
Lobel, S.A. et al. (1984) The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody.
Immunology 51: 35-43.
References for MHC Class II DQ antibody
-
Spieker-Polet, H. et al. (1990) Rabbit major histocompatibility complex. IV. Expression of major histocompatibility complex class II genes.
J Immunogenet. 17 (1-2): 123-32. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology 77: 106-8. -
Idogawa, H. et al. (1997) Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits.
Scand J Immunol. 46: 572-80. -
Andersen, H.O. et al. (1999) Effect of cyclosporine on arterial balloon injury lesions in cholesterol-clamped rabbits: T lymphocyte-mediated immune responses not involved in balloon injury-induced neointimal proliferation.
Arterioscler Thromb Vasc Biol. 19: 1687-94. -
Matsumura, T. et al. (1999) Suppression of atherosclerotic development in Watanabe heritable hyperlipidemic rabbits treated with an oral antiallergic drug, tranilast.
Circulation 99 (7): 919-24. -
Alt, C. et al. (2009) Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.
Curr HIV Res. 7: 639-49. -
Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
Biotechnol Prog. 26 (6): 1749-58. -
Shen, W. et al. (2013) Osteoarthritis prevention through meniscal regeneration induced by intra-articular injection of meniscus stem cells.
Stem Cells Dev. 22 (14): 2071-82.
View The Latest Product References
-
Sun, F. et al. (2023) Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step.
Bioeng Transl Med. 8 (4): e10534.
MCA811GA
1610
166443
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