MHC Class II DQ antibody | 2C4

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Mouse anti Rabbit MHC Class II DQ

Product Type
Monoclonal Antibody
Clone
2C4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA811GA C F IP 0.1 mg
Mouse anti rabbit MHC class II monoclonal antibody, clone 2C4 recognizes the class II RLA-DQ molecule. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rabbits, this complex is referred to as the rabbit leukocyte antigen (RLA) region. There are 3 major MHC class II proteins encoded by the RLA which are RLA DP, RLA DQ and RLA DR.

Mouse anti rabbit MHC class II recognizes the RLA DQ molecule, expressed by antigen presenting cells, B cells and monocytes.

This antibody is reported to inhibit the mixed lymphocyte reaction by 80%. Immunoprecipitation studies yield three bands of molecular weight 28, 31 and 35 kDa.

Product Details

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit spleen cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3.X63. Ag8.653 mouse myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II DQ antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for MHC Class II DQ antibody

  1. Lobel, S.A. et al. (1984) The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody.
    Immunology 51: 35-43.
  2. Spieker-Polet, H. et al. (1990) Rabbit major histocompatibility complex. IV. Expression of major histocompatibility complex class II genes.
    J Immunogenet. 17 (1-2): 123-32.
  3. Matsumura, T. et al. (1999) Suppression of atherosclerotic development in Watanabe heritable hyperlipidemic rabbits treated with an oral antiallergic drug, tranilast.
    Circulation 99 (7): 919-24.
  4. Yuan, T. et al. (2010) Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel.
    Biotechnol Prog. 26 (6): 1749-58.
  5. Andersen, H.O. et al. (1999) Effect of cyclosporine on arterial balloon injury lesions in cholesterol-clamped rabbits: T lymphocyte-mediated immune responses not involved in balloon injury-induced neointimal proliferation.
    Arterioscler Thromb Vasc Biol. 19: 1687-94.
  6. Alt, C. et al. (2009) Increased CCL2 expression and macrophage/monocyte migration during microbicide-induced vaginal irritation.
    Curr HIV Res. 7: 639-49.
  7. Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
    Immunology 77: 106-8.
  8. Idogawa, H. et al. (1997) Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits.
    Scand J Immunol. 46: 572-80.
  9. Shen, W. et al. (2013) Osteoarthritis prevention through meniscal regeneration induced by intra-articular injection of meniscus stem cells.
    Stem Cells Dev. 22 (14): 2071-82.