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Light Chain antibody | SB62a

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Anti Rabbit Light Chain Antibody, clone SB62a thumbnail image 1
Anti Rabbit Light Chain Antibody, clone SB62a gallery image 1
HeLa (lane 2 and 5) and HepG2 (lane 3 and 6) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 4. Rabbit anti Human GAPDH (AHP1628) was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP (MCA6003P) was used as the secondary antibody at dilutions of 1/5000 (lanes 2 and 3) and 1/10000 (lanes 5 and 6). Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure.

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WB

Mouse anti Rabbit Light Chain:HRP

Product Type
Monoclonal Antibody
Clone
SB62a
Isotype
IgG2b
Specificity
Light Chain

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Product Code Applications Pack Size List Price Your Price Qty
MCA6003P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.5 ml loader
List Price Your Price
loader

Mouse anti Rabbit light chain antibody, clone SB62a only binds to the light chain of rabbit IgG and does not bind to the heavy chain. As such it can be used as a secondary antibody in western blotting, EIA and immunoprecipitation. This antibody is not recommended for the direct detection of reduced or denatured rabbit chains on western blots.

Target Species
Rabbit
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% Glycerol
Immunogen
Rabbit light chain
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000 1/8000
Western Blotting 1/1000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

MCA6003P

155142 157741 180730

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