CD45 antibody | L12/201





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F IF ResetMouse anti Rabbit CD45
- Product Type
- Monoclonal Antibody
- Clone
- L12/201
- Isotype
- IgG1
- Specificity
- CD45
Mouse anti Rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200. Mouse anti Rabbit CD45 antibody, clone L12/201 shows pan leucocyte reactivity by flow cytometry and immunohistochemistry. Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa. |
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Glycoproteins isolated from the T cell line, RL-5.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/100 |
Immunofluorescence | ![]() |
||
Immunohistology - Frozen | ![]() |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
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Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Description | Rabbit F(ab')2 anti Mouse IgG:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Description | Mouse IgG1 Negative Control |
References for CD45 antibody
-
Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
J Exp Med. 157 (1): 34-46. -
Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
Mol Immunol. 21 (1): 95-103. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77 (1): 106-8. -
Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
J Pathol. 170 (3): 315-20. -
Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
Arterioscler Thromb Vasc Biol. 21: 220-6. -
Mackenzie, S.M. et al. (2006) Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3.
Biol Reprod. 74: 511-21. -
Davis, W.C. & Hamilton, M.J. (2008) Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules.
J Vet Sci. 9 (1): 51-66. -
Liang, H. et al. (2009) Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 15: 1690-9.
View The Latest Product References
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Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
Transplantation. 89: 409-16. -
Vašíček, J et al. (2014) Basic Blood Analysis of Rabbits Immunized with Vaccine Against Myxomatosis.
Proc Int Symp Anim Sci 2014: 411-6. -
Vasicek, J. et al. (2015) Determination of Lymphocyte Subset Distribution in the Peripheral Blood of Rabbits Immunized with CFA.
Int Symp Anim Sci UDC:639.112 pp. 226-31. -
Sijnave, D. et al. (2015) Inhibition of Rho-Associated Kinase Prevents Pathological Wound Healing and Neovascularization After Corneal Trauma.
Cornea. 34 (9): 1120-9. -
Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Ondruska, L.. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
J Cent Eur Agric.18 (1): 226-34. -
Kovac, M. et al. (2017) Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells.
Biotechnol Prog. 33 (6): 1601-13. -
Vašíček, J et al. (2018) The Efficiency of Immunomagnetic Sorting of Rabbit Bone Marrow Celld for the Establishment of Mesenchymal Stem Cell Culture.
J Microbiol Biotech Food Sci. 8 (3): 890-2. -
Barth, H. et al. (2019) Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
Graefes Arch Clin Exp Ophthalmol. 257 (4): 769-83. -
Kulikova, B. et al. (2019) Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.
Acta Histochem. 121 (4): 491-9. -
Desando, G. et al. (2021) Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care.
Stem Cell Rev Rep. 17 (4): 1362-73. -
Adly, H. et al. (2021) Repopulation of multipotent stem cells derived from adult male rabbits on a polycaprolactone scaffold: an in vitro. study
Al-Azhar International Medical Journal. 2 (11): 37-42. -
Wee, J.H. et al. (2022) Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration.
Biomater Res. 26 (1): 79. -
Mastrolia, I. et al. (2022) Autologous Marrow Mesenchymal Stem Cell Driving Bone Regeneration in a Rabbit Model of Femoral Head Osteonecrosis.
Pharmaceutics. 14(10):2127.
- Synonyms
- LCA
- RRID
- AB_10961760
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