CD45 antibody | L12/201
Mouse anti Rabbit CD45 antibody, clone L12/201 (MCA808GA) used for the evaluation of leukocyte infiltrates in conjunctival associated lymphoid tissue by flow cytometry and immunofluorescence microscopy.
Image caption:
Conjunctival imprints evaluated by flow cytometry for CD45-positive cells after multiple instillations at H4, and assessed after cytospin centrifugation. The inflammatory marker clearly showed the highest expression in BAC-containing eye drops (A). PBS- and LCEm- instilled groups had only a basal level of expression. CD45-positive cells (green) after instillations of BAC+ latanoprost (B) were viewed after propidium iodide staining (red) and cytospin centrifugation. The scale bar indicates 50 μm. The asterisk indicates a p<0.05 compared with BAC+latanoprost-instilled group. The double asterisk indicates a p<0.02 compared with BAC+latanoprost-instilled group.
From: Liang H, Baudouin C, Faure MO, Lambert G, Brignole-Baudouin F.
Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 2009 Aug 25;15:1690-9.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit CD45 antibody, clone L12/201 (MCA808GA) used for the demonstration of leukocytes in lapine retinal sections by immunofluorescence on cryostat sections.
Image caption:
Cryosections of rabbit retina co-labeled with CD45 (pan-leukocyte) (green) and Galactein-3 (mainly microglia) (red): routine vitrectomy with BSS after 1 day (a), 1 week (b), and 1 month (c). Healaflow® treatment after 1 day (d), 1 week (e), and 1 month (f). Silicone oil treatment after 1 day (composite; representative retina left, clustering of CD45+ cells at the optic nerve head right) (g), 1 week (h), and 1 month (I). Bio-Alcamid® treatment after 1 week (j), and 1 month with arrow indicating multi-nucleated giant cell (k). Vitrectomy with significant complications and BSS in eyes with retinal break after 1 day (l), retinal break after 1 week (m), and retinal break after 1 month (composite; representative retina left, localized infiltration of CD45+ cells at a retinal break right) (n). GLC ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer. Scale bar is 50μm
From: Barth H, Crafoord S, Arnér K, Ghosh F.
Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
Graefes Arch Clin Exp Ophthalmol. 2019 Apr;257(4):769-83.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200. Mouse anti Rabbit CD45 antibody, clone L12/201 shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.
Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Glycoproteins isolated from the T cell line, RL-5.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD45 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
|
|
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD45 antibody
-
Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
J Exp Med. 157 (1): 34-46. -
Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
Mol Immunol. 21 (1): 95-103. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77 (1): 106-8. -
Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
J Pathol. 170 (3): 315-20. -
Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
Transplantation. 89: 409-16. -
Mackenzie, S.M. et al. (2006) Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3.
Biol Reprod. 74: 511-21. -
Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
Arterioscler Thromb Vasc Biol. 21: 220-6. -
Liang, H. et al. (2009) Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 15: 1690-9. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85: 44-54. -
Kuznetsov, S.A. et al. (2001) Circulating skeletal stem cells.
J Cell Biol. 153: 1133-40. -
Sijnave, D. et al. (2015) Inhibition of Rho-Associated Kinase Prevents Pathological Wound Healing and Neovascularization After Corneal Trauma.
Cornea. 34 (9): 1120-9. -
Kovaacute;č, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
Slovak J Anim Sci., 49,(2): 62–67. -
Ondruska, L.. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Davis, W.C. & Hamilton, M.J. (2008) Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules.
J Vet Sci. 9 (1): 51-66. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Miyahara, T. et al. (2013) D-series resolvin attenuates vascular smooth muscle cell activation and neointimal hyperplasia following vascular injury.
FASEB J. 27 (6): 2220-32. -
Vasicek, J. et al. (2015) Determination of Lymphocyte Subset Distribution in the Peripheral Blood of Rabbits Immunized with CFA.
Int Symp Anim Sci UDC:639.112 pp. 226-31. -
Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
Journal of Central European Agriculture. 18 (1): 226-34. -
Vašíček, J et al. (2018) The Efficiency of Immunomagnetic Sorting of Rabbit Bone Marrow Celld for the Establishment of Mesenchymal Stem Cell Culture.
Journal of Microbiology, Biotechnology and Food Sciences. 8 (3): 890-2. -
Vašíček, J et al. (2014) Basic Blood Analysis of Rabbits Immunized with Vaccine Against Myxomatosis.
Proc Int Symp Anim Sci 2014: 411-6. -
Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Barth, H. et al. (2019) Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
Graefes Arch Clin Exp Ophthalmol. 257 (4): 769-83. -
Kovac, M. et al. (2017) Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells.
Biotechnol Prog. 33 (6): 1601-13.
