CD45 antibody | L12/201
Mouse anti Rabbit CD45 antibody, clone L12/201 (MCA808GA) used for the evaluation of leukocyte infiltrates in conjunctival associated lymphoid tissue by flow cytometry and immunofluorescence microscopy.
Image caption:
Conjunctival imprints evaluated by flow cytometry for CD45-positive cells after multiple instillations at H4, and assessed after cytospin centrifugation. The inflammatory marker clearly showed the highest expression in BAC-containing eye drops (A). PBS- and LCEm- instilled groups had only a basal level of expression. CD45-positive cells (green) after instillations of BAC+ latanoprost (B) were viewed after propidium iodide staining (red) and cytospin centrifugation. The scale bar indicates 50 μm. The asterisk indicates a p<0.05 compared with BAC+latanoprost-instilled group. The double asterisk indicates a p<0.02 compared with BAC+latanoprost-instilled group.
From: Liang H, Baudouin C, Faure MO, Lambert G, Brignole-Baudouin F.
Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 2009 Aug 25;15:1690-9.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit CD45 antibody, clone L12/201 (MCA808GA) used for the demonstration of leukocytes in lapine retinal sections by immunofluorescence on cryostat sections.
Image caption:
Cryosections of rabbit retina co-labeled with CD45 (pan-leukocyte) (green) and Galactein-3 (mainly microglia) (red): routine vitrectomy with BSS after 1 day (a), 1 week (b), and 1 month (c). Healaflow® treatment after 1 day (d), 1 week (e), and 1 month (f). Silicone oil treatment after 1 day (composite; representative retina left, clustering of CD45+ cells at the optic nerve head right) (g), 1 week (h), and 1 month (I). Bio-Alcamid® treatment after 1 week (j), and 1 month with arrow indicating multi-nucleated giant cell (k). Vitrectomy with significant complications and BSS in eyes with retinal break after 1 day (l), retinal break after 1 week (m), and retinal break after 1 month (composite; representative retina left, localized infiltration of CD45+ cells at a retinal break right) (n). GLC ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer. Scale bar is 50μm
From: Barth H, Crafoord S, Arnér K, Ghosh F.
Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
Graefes Arch Clin Exp Ophthalmol. 2019 Apr;257(4):769-83.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit CD45 antibody, clone L12/201 (MCA808GA) used to evaluate levels of CD45 expression on rabbit stromal vascular fraction cells by flow cytometry.
Image caption:
A. The forward scatter (FSC) / side scatter (SSC) gating graph in the eSVF group. Cells in the black circle represent the cell population analysed. B-C Histograms of isotype controls IgG1 and IgG2a. D-K. Histograms reporting the percentage of positivity (P2) of a representative specimen in the eSVF group for the following markers: CD-44, CD-90, CD-105, CD-146, CD-13, CD-14, CD-45 and CD-68.
From: Desando G, Bartolotti I, Cattini L, Tschon M, Martini L, Fini M, Schiavinato A, Soranzo C, Grigolo B.
Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care.
Stem Cell Rev Rep. 2021 Jan 19 [Epub ahead of print].
doi: 10.1007/s12015-020-10099-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200. Mouse anti Rabbit CD45 antibody, clone L12/201 shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.
Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Glycoproteins isolated from the T cell line, RL-5.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD45 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
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| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
|
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| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD45 antibody
-
Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
J Exp Med. 157 (1): 34-46. -
Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
Mol Immunol. 21 (1): 95-103. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77 (1): 106-8. -
Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
J Pathol. 170 (3): 315-20. -
Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
Transplantation. 89: 409-16. -
Mackenzie, S.M. et al. (2006) Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3.
Biol Reprod. 74: 511-21. -
Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
Arterioscler Thromb Vasc Biol. 21: 220-6. -
Liang, H. et al. (2009) Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 15: 1690-9. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85: 44-54. -
Kuznetsov, S.A. et al. (2001) Circulating skeletal stem cells.
J Cell Biol. 153: 1133-40. -
Sijnave, D. et al. (2015) Inhibition of Rho-Associated Kinase Prevents Pathological Wound Healing and Neovascularization After Corneal Trauma.
Cornea. 34 (9): 1120-9. -
Kováč, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
Slovak J Anim Sci., 49,(2): 62–67. -
Ondruska, L.. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Davis, W.C. & Hamilton, M.J. (2008) Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules.
J Vet Sci. 9 (1): 51-66. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Miyahara, T. et al. (2013) D-series resolvin attenuates vascular smooth muscle cell activation and neointimal hyperplasia following vascular injury.
FASEB J. 27 (6): 2220-32. -
Vasicek, J. et al. (2015) Determination of Lymphocyte Subset Distribution in the Peripheral Blood of Rabbits Immunized with CFA.
Int Symp Anim Sci UDC:639.112 pp. 226-31. -
Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
Journal of Central European Agriculture. 18 (1): 226-34. -
Vašíček, J et al. (2018) The Efficiency of Immunomagnetic Sorting of Rabbit Bone Marrow Celld for the Establishment of Mesenchymal Stem Cell Culture.
Journal of Microbiology, Biotechnology and Food Sciences. 8 (3): 890-2. -
Vašíček, J et al. (2014) Basic Blood Analysis of Rabbits Immunized with Vaccine Against Myxomatosis.
Proc Int Symp Anim Sci 2014: 411-6. -
Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Barth, H. et al. (2019) Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
Graefes Arch Clin Exp Ophthalmol. 257 (4): 769-83. -
Kovac, M. et al. (2017) Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells.
Biotechnol Prog. 33 (6): 1601-13. -
Desando, G. et al. (2021) Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care.
Stem Cell Rev Rep. Jan 19 [Epub ahead of print]. -
Kulikova, B. et al. (2019) Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.
Acta Histochem. 121 (4): 491-9.
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