CD45 antibody | L12/201



Immunoprecipitation was achieved by cross linking antibody to the labelled cell surface yielding a protein migrating by gel electrophoresis at a molecular mass of ~200 kDa.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Glycoproteins isolated from the T cell line, RL-5.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD45 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Product Specific References
References for CD45 antibody
-
Jackson, S. et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.
J Exp Med. 157 (1): 34-46. -
Wilkinson, J.M. et al. (1984) Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.
Mol Immunol. 21 (1): 95-103. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77 (1): 106-8. -
Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
J Pathol. 170 (3): 315-20. -
Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
Transplantation. 89: 409-16. -
Mackenzie, S.M. et al. (2006) Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3.
Biol Reprod. 74: 511-21. -
Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
Arterioscler Thromb Vasc Biol. 21: 220-6. -
Liang, H. et al. (2009) Comparison of the ocular tolerability of a latanoprost cationic emulsion versus conventional formulations of prostaglandins: an in vivo toxicity assay.
Mol Vis. 15: 1690-9. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85: 44-54. -
Kuznetsov, S.A. et al. (2001) Circulating skeletal stem cells.
J Cell Biol. 153: 1133-40. -
Sijnave, D. et al. (2015) Inhibition of Rho-Associated Kinase Prevents Pathological Wound Healing and Neovascularization After Corneal Trauma.
Cornea. 34 (9): 1120-9. -
Kovaacute;č, M. et al. (2016) Cryopreservation of Amniotic Fluid Stem Cells Derived From Zobor Rabbits.
Slovak J Anim Sci., 49,(2): 62–67. -
Ondruska, L.. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Davis, W.C. & Hamilton, M.J. (2008) Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules.
J Vet Sci. 9 (1): 51-66. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Miyahara, T. et al. (2013) D-series resolvin attenuates vascular smooth muscle cell activation and neointimal hyperplasia following vascular injury.
FASEB J. 27 (6): 2220-32. -
Vasicek, J. et al. (2015) Determination of Lymphocyte Subset Distribution in the Peripheral Blood of Rabbits Immunized with CFA.
Int Symp Anim Sci UDC:639.112 pp. 226-31. -
Kováč, M. et al. (2017) Phenotype and ultrastructure of stem cells derived from amniotic fluid of Nitra rabbit
Journal of Central European Agriculture. 18 (1): 226-34. -
Vašíček, J et al. (2018) The Efficiency of Immunomagnetic Sorting of Rabbit Bone Marrow Celld for the Establishment of Mesenchymal Stem Cell Culture.
Journal of Microbiology, Biotechnology and Food Sciences. 8 (3): 890-2. -
Vašíček, J et al. (2014) Basic Blood Analysis of Rabbits Immunized with Vaccine Against Myxomatosis.
Proc Int Symp Anim Sci 2014: 411-6. -
Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine
Veterinární Medicína. 61 (No. 10): 571-6. -
Barth, H. et al. (2019) Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model.
Graefes Arch Clin Exp Ophthalmol. 257 (4): 769-83. -
Kovac, M. et al. (2017) Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells.
Biotechnol Prog. 33 (6): 1601-13. -
Desando, G. et al. (2021) Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care.
Stem Cell Rev Rep. Jan 19 [Epub ahead of print].
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