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L-LACTATE Dehydrogenase antibody | AbD14008_hIgG1

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Anti Plasmodium falciparum LDH Antibody, clone AbD14008_hIgG1 gallery image 1
Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum LDH (HCA158) was used as the coating antibody. The antigen (recombinant LDH from Plasmodium falciparum in PBST) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium Falciparum LDH.

Human anti Plasmodium falciparum LDH

Product Type
Monoclonal Antibody
Clone
AbD14008_hIgG1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
HCA158
Datasheet
E
MSDS
0.1 mg loader

Human anti Plasmodium falciparum LDH, clone AbD14008 recognizes LDH (L-lactose dehydrogenase) from the Malaria causing protozoan species Plasmodium falciparum.

Human anti Plasmodium falciparum LDH, clone AbD14008 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

Human anti Plasmodium falciparum LDH, clone AbD14008 (HCA158) is designed for use as a capture antibody with HCA157P as the detection antibody in sandwich assay development.

Human anti Plasmodium falciparum LDH, clone AbD14008 (HCA158) does not cross react with LDH from Plasmodium vivax.

Product Details

Target Species
Protozoan
Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL ® phage display library and expressed in a human cell line. This product is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant LDH fusion protein from Plasmodium falciparum.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P13774
GO Terms
GO:0004459 L-lactate dehydrogenase activity
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of L-LACTATE Dehydrogenase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used for indirect ELISA or as a capture reagent in a sandwich ELISA together with HCA157P as the detection reagent.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
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