L-LACTATE Dehydrogenase antibody | AbD14001_hIgG1

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Anti Plasmodium falciparum LDH Antibody, clone AbD14001_hIgG1 thumbnail image 1

Anti Plasmodium falciparum LDH Antibody, clone AbD14001_hIgG1 gallery image 1 — Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti *Plasmodium falciparum* LDH (HCA158) was used as the coating antibody. The antigen (recombinant LDH from *Plasmodium falciparum* in PBST) was titrated using the indicated concentrations. Detection was performed with ECL-labeled Human anti *Plasmodium falciparum* LDH

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Human anti Plasmodium falciparum LDH:HRP

Product Type: Monoclonal Antibody
Clone: AbD14001_hIgG1
Isotype: IgG1
Specificity: L-LACTATE Dehydrogenase

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Human anti Plasmodium falciparum LDH antibody, clone AbD14001 specifically recognizes LDH (L-lactose dehydrogenase) from the Malaria causing protozoan species Plasmodium falciparum.

Clone AbD14001 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

When conjugated this clone is suitable for use as a detection antibody with HCA158 as the capture antibody in a sandwich assay.

This clone is not cross reactive with LDH from Plasmodium vivax. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 71 °C

Target Species: Protozoan
Product Form: Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: HKB-11
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Recombinant LDH fusion protein from Plasmodium falciparum
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA: This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with HCA158 as the capture reagent.

RRID: AB_10926388
UniProt: P13774
GO Terms: GO:0004459 L-lactate dehydrogenase activity; GO:0006096 glycolysis; GO:0055114 oxidation-reduction process
Licensed Use: For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.