L-LACTATE Dehydrogenase antibody | AbD14001_hIgG1

Human anti Plasmodium falciparum LDH:HRP

Product Type
Monoclonal Antibody
Clone
AbD14001_hIgG1
Isotype
IgG1
Specificity
L-LACTATE Dehydrogenase

Product Code Applications Pack Size List Price Your Price Qty
HCA157P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human anti Plasmodium falciparum LDH antibody, clone AbD14001 specifically recognizes LDH (L-lactose dehydrogenase) from the Malaria causing protozoan species Plasmodium falciparum.

Clone AbD14001 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

When conjugated this clone is suitable for use as a detection antibody with HCA158 as the capture antibody in a sandwich assay.

This clone is not cross reactive with LDH from Plasmodium vivax. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 71 °C

Target Species
Protozoan
Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Recombinant LDH fusion protein from Plasmodium falciparum
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with HCA158 as the capture reagent.

RRID
AB_10926388
UniProt
P13774
GO Terms
GO:0004459 L-lactate dehydrogenase activity
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.